AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (October 2019) – FOS-03 MANUSCRIPT

FOR ERP USE ONLY DO NOT DISTRIBUTE

1

Table 13. Elution program used by lab 12

Flow rate, mL/min

Water, % A

250 mM NaOH, % B

650 mM NaOAc, % C

Time, min

0

1.0 1.0 1.0 1.0 1.0 1.0 1.0

90 90 70 30 30 90 90

10 10 30 30 30 10 10

0 0 0

12 15 22 24 26 35

40 40

0 0

2 3 4

B

A

5 Figure 7. Example of chromatograms obtained by lab 12, using CarboPac PA1 column 6 (4 x 250 mm) without post-column addition of NaOH and elution program described in Table 7 13. A: Calibration standard solution. B: Child formula powder containing fructans.

8 9

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Recommendation

The performance of the method largely meets the requirements of the SMPR and has 11 demonstrated excellent resilience to adaptations made by the MLT participants. In addition, 12 satisfactory accuracy was also demonstrated by good agreement of results on SRM sample with 13 available reference value. First Action Official Method 2016.14 has thus demonstrated to be fit for 14 purpose in determining fructans in infant formula and adult/pediatric nutritional formula. 15

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