Biophysical Society Thematic Meeting| Lima 2019

Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level

Sunday Speaker Abstracts

A SINGLE-MOLECULE VIEW OF MAMMALIAN DNA DOUBLE-STRAND BREAK REPAIR Eli Rothenberg New York University, New York, NY, USA Chromosomal breaks (DSBs) are the most genotoxic type of DNA damage, and are caused by Ionization Radiation (IR), chemotherapeutic agents as well as from normal cellular processes. The induction of DSBs result in the recruitment of various DNA repair and signaling factors to form microscopic repair foci. Within these foci different repair factors function as a multi component repair factory, supporting the regulation and progression of the DSB repair process. The organization and dynamics of the different factors within repair foci and the precise mechanisms by which they promote repair are still subject to much uncertainty, posing critical constraints to our understanding of the DSB repair processes. In our recent and ongoing efforts, we have developed and utilized various single-molecule methods, including biochemical assays, super-resolution microscopy and live cell imaging to address fundamental gaps in our knowledge of mammalian DNA repair pathways. Our studies revealed distinctive features of the molecular kinetics along with unique interaction landscapes that encompasses interactions at the DNA-protein level and chromatin level, which together mediate and regulated the DSB repair process.

IMAGING AT THE GENOMIC-SCALE: FROM 3D ORGANIZATION OF THE GENOMIC DNA TO CELL ATLAS OF COMPLEX TISSUES Xiaowei Zhuang Harvard University, Cambridge, MA, USA No Abstract

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