Biophysical Society Thematic Meeting| Lima 2019

Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level

Poster Abstracts

8-POS Board 8 INDUSTRIAL ENZYME ENGINEERING: A JOURNEY FROM SINGLE MOLECULES TO TONS Philip M Lund 1,2 ; Søren S Nielsen 2 ; Amalie S Kallenbach 2 ; Nikos Hatzakis 2 ; Sune M. Christensen 1 ; Enzymes are ubiquitous in biology and underpin all forms of life as we know it. Protein engineering can be applied to repurpose naturally occurring enzymes to act as catalysts in industrial processes and as functional constituents in consumer products. Enzyme performance in application is a macroscopic phenomenon emerging from a complex cascade of events transcending length scales from individual molecules to the ensemble. For heterogenous reactions, comprising the bulk of commercially interesting processes, enzyme performance includes, e.g., surface binding, orientation, conformational changes, diffusion, stability, catalytic activity and colloidal effects. The ideal enzyme product should excel at all these levels. Molecular scale in-depth understanding of structure-function relationships is a critical element in engineering such an enzyme. However, in most cases unambiguous information of this kind is extremely difficult to obtain by conventional approaches. Single molecule techniques can provide answers to many of these problems. I will discuss our efforts in this area with emphasis on a recent single particle tracking study of engineered lipase mobility on a triglyceride substrate. 1 Novozymes A/S, Protein Engineering, Lyngby, Denmark 2 University of Copenhagen, Chemistry, Copenhagen, Denmark

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