Biophysical Society Thematic Meeting| Lima 2019

Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level

Poster Abstracts

11-POS Board 11 OBSERVING DNA FLEXIBILITY USING TETHERED PARTICLE MICROSCOPY Chamaree de Silva 1 ; Shruthi Vikraman 1 ; Erin Moore 1 ; 1 Mercer University, Department of Physics, Macon, GA, USA Tethered Particle Microscopy is a single-molecule biophysical technique that is used to study DNA flexibility by fixing one end of the strand to a microscope slide and attaching a one micrometer synthetic bead to the other end. Using an Olympus CX31 microscope and a digital microscope imager, the precise movement of the bead is recorded. This movement is then monitored using a particle tracking software to determine the characteristics of the DNA molecule. This in turn provides insight into the DNA's flexibility and looping. In this experiment, 2D displacement of the bead over a few minutes of each particle was recorded to see the range of motion of tethered lambda-DNA in real time.

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