Biophysical Society Thematic Meeting| Lima 2019

Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level

Poster Abstracts

13-POS Board 13 AFM TIPS FUNCTIONALIZED WITHETHYLENEDIAMINEBYPECVD AND THEINTERACTION OF GLUTARALDEHYDECROSSLINKED SURFACES IN AQUEOUS SOLUTIONS Claudio Victor dos Santos Junior 1,2 ; 1 UFRJ, dio victor Dos santos junior ; Institutions: UFRJ, materials science and engineering, RJ, Brazil 2 UFRJ, Biophysical, RJ, Brazil Atomic force microscopy(afm) force distance curves were used to evaluate the efficiencyof using amino precursor coatings produced byplasma-enhanced chemical vapor deposition (pecvd) to immobilize glutaraldehyde (ga) on silicon nitrate substrates. Deposition was performed in a capacitive reactor using ethylenediamine (eda) as precursor as it contain both carbon as well as amine groups, c h (nh ) . Coatings were characterized byfast fourier infrared spectroscopy(ftir) and showed characteristic amine peaks. Glutaraldehyde was immobilized on coated substrates at different concentrations and the crosslinking interaction forces between the surface and the tip were evaluated bymeasuring the adhesion force using afm force distance curves and characteristic covalent force occurrence probability. Increased as the ga concentration increases. Topographic images of eda functionalized silicon wafers were acquired before and after deposition of glutaraldehyde, in air and in pbs solution. Images showed a drastic decrease of the roughness in air. All results indicate that amine surfaces functionalization performed bypecvd with eda is a promisor path for biorecognition surfaces in biomedical and biosensors applications among others

49