Biophysical Society Thematic Meeting| Lima 2019

Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level

Poster Abstracts

24-POS Board 24 SILVER BUT NOT GOLD NANOPARTICLES INDUCE CHANGES IN GLOBAL DNA METHYLATION AND DNA DAMAGE IN TWO TUMOR CELL LINES Lucyna Kapka-Skrzypczak 1,2 ; Barbara Sochanowicz 3 ; Sylwia Meczynska-Wielgosz 3 ; Maria Wojewódzka 3 ; Marcin Kruszewski 1,2,3 ; 1 University of Information Technology and Management, Department of Medical Biology and Translational Research, Rzeszów, Poland 2 Institute of Rural Health, Department of Molecular Biology and Translational Research, Lublin, Poland 3 Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and Biological Dosimetry, Warsaw, Poland Nanoparticles (NPs) are defined as particles having all dimensions of 100 nm or less. Metallic NPs are used in an increasing number of applications in industry, science and medicine. Among metallic NPs foreseen to be widely used in medicine are gold nanoparticles (AuNPs) due to their low toxicity and silver NPs (AgNPs), due to their strong antimicrobial activity. The aim of this study was to compare effect of AgNPs and gold NPs (AuNPs) on global DNA methylation and formation of DNA damage in two cell lines, A2780 and 4T1, a widely used model of human ovarian carcinoma and murine mammary carcinoma, respectively. The cells were exposed to AgNPs coated with citrate (AgNPs(cit) or PEG (AgNPs(PEG), or AuNPs and global DNA methylation and formation of DNA damage was investigated. AgNPs but AuNPs decreased global DNA methylation and increased formation of DNA lesions in both cell lines. The effect was dependent on type of NPs used, its coating and cell line used. In conclusion, the effects of NPs strongly depends on NPs nature and cellular context. Epigenetic changes observed upon action of AgNPs may play a crucial role in NPs-induced changes in protein expression. This work was supported by National Science Centre grant No. 2014/15/B/NZ7/01036 (MK, LKS), statutory funding for INTC (BS, SMW, MW) and IRH (MK, LKS).

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