Biophysical Society Thematic Meeting| Lima 2019

Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level

Poster Abstracts

26-POS Board 26 POSTTRANSLATIONAL MODIFICATIONS OF HISTONE H3 IN TUMOR CELLS TREATED WITH SILVER AND GOLD NANOPARTICLES Marcin Kruszewski 1,2,3 ; Barbara Sochanowicz 2 ; Lucyna Kapka-Skrzypczak 1,3 ; 1 University of Information Technology and Management, Department of Medical Biology and Translational Research, Rzeszów, Poland 2 Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and Biological Dosimetry, Warsaw, Poland 3 Institute of Rural Health, Department of Molecular Biology and Translational Research, Lublin, Poland Nanoparticles (NPs) are defined as particles having all dimensions less than 100 nm. The small size of NPs results in unusual chemical and physical properties different from a bulk material. One of the most generally used NPs are silver nanoparticles (AgNPs). First of all, they have strong antimicrobial activity and are widely used in various medical and general applications, among others, in cosmetics, odour resistant textiles etc. The aim of this study was to compare effect of AgNPs and gold NPs (AuNPs) on histone H3 posttranslational modifications. Histone molecule posttranscriptional modifications are responsible for chromatin compaction and repackaging. Two cell lines: A2780 and 4T1. Both cell lines are widely used as a model cells of human ovarian carcinoma and murine mammary carcinoma, respectively. The cells were exposed to AgNPs coated with citrate (AgNPs(cit) or PEG (AgNPs(PEG), or AuNPs and thereafter the histone H3 acetylation on Lys9 and H3 methylation on Lys4, Lys9, Lys29 was investigated. All NPs tested decreased H3 methylation, while no effect was observed for H3 acetylation. Modification of histone H3 methylation dependent on type of NPs used, its coating, site of methylation and cell line used. In conclusion, whereas a simple comparison of different NPs in one particular cell line gave similar results, the comparison between cell lines revealed additional factors that might affect cellular response to nanoparticles. This work was supported by National Science Centre grant No. 2014/15/B/NZ7/01036 (MK, LKS), statutory funding for INTC (BS) and IRH (MK, LKS).

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