Biophysical Society Thematic Meeting| Lima 2019

Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level

Poster Abstracts

55-POS Board 55 WORKING FROM A DISTANCE: ENGINEERING ALLOSTERIC CONTROL ON PDZ3 DOMAIN SELECTIVITY FROM ZO-1 PROTEIN THROUGH DOMAIN FUSION Jiri Vondrasek 1 ; Kristyna Bousova 1 ; Konstantinos Tripsianes 2 ; 1 Institute of Organic Chemistry and Biochemistry, Bioinformatics, Prague, Czech Republic 2 CEITEC, Brno, Czech Republic A central paradigm within structural biology is the concept of domains. A particular domain can be identified and assigned to specific functional or structural properties. Despite the fact that there is a high but limited number of protein domains, they appear in countless combinations. It is plausible to expect that organization of domains can modulate a function via allostery. We present results of our study on PDZ domain to capture external allostery exerted by adjacent domains by means of combination of theoretical and experimental methods. Our study of protein chimeras consisting of PDZ3 from the ZO-1 protein (homo sapiens) and various fusion domains will help to understand the mechanism of allosteric regulation in PDZ3 specificity by means of the character, position or size of the attached domain. We also provide an overview of molecular design on selected chimeras and methodological background for their theoretical description. These results are compared with biophysical and structural characterizations to elucidate the nature of the allostery in these model systems.

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