Amino-01 (July 2018)

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals

Appendix E: reproducibility performance requirements for each analyte/sample pair according to the concentration of the analyte in the reconstituted product. Appendix F: intermediate reproducibility data obtained with the SPIFAN II SLV kit products, color-coded with respect to reproducibility requirement values. Appendix G: recovery performance requirements for each analyte/sample pair according to the concentration of the analyte in the reconstituted product. Appendix H: recovery data obtained with the SPIFAN II SLV kit products, color-coded with respect to performance requirement values. Appendix I: example chromatograms for selected concentrations of the calibration curve and SPIFAN II SLV kit products. Appendix J: Additional information requested by AOAC SPIFAN ERP during mid-year AOAC meeting (March 2017). REFERENCES Barkholt V. and Jensen A. L. (1989). Amino acid analysis: Determination of cysteine plus half- cystine in proteins after hydrochloric acid hydrolysis with a disulphide compound as additive. Analytical Biochemistry 177, 318-322. 6

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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