Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendices A-I

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals -– APPENDICES (A to I) Appendix B : average values of the SPIFAN II SLV kit products in mg/100 g product.

SPIFAN II SLV kit product

Analyte

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17 28 79 36 28 5

18

19

His

371 N/A 884 572 327

407 N/A 561 949 426 717 581 553 839 202 372 1078 1272 1105 1830 1783 2196

127 N/A 308 709 106 503 253 219 519 491 268 139 326 274 584 259 1238 59

196 N/A 449 244 142 581 327 241 841 595 398 196 489 381 725 397 1789 27

27

311

200

287

319

245

404 N/A 639 785

342

398

252

231

303

129

189

Tau Ser Arg Gly Asp Glu Thr Ala Pro Lys Tyr

N/A

37

42

47

32

42

73

36

34

43

58

17

20

82 36 28

706 390 243

644 269 222

660 850 509

788 417 270

593 317 240

801 534 319

521 934 440

601 332 227

597 307 203

677 906 524

312 708 106 492 253 215 511 485 271 142 324 270 575 261 57

462 237 141 583 329 240 836 598 403 200 478 372 723 388 25

1380 1292 2191

1423 3272

156 303

1136 2749

1435 2174 838 626 698 334 302 230 621 670 1184 1245

1561 2512

1331 3008

1092 1888

1324 3014

1764 2159 710 563 548 860 215 387 1004 1283 1121 1807

1145 2236 591 478 838 474 211 267 667 620 1014 1200

1025 2200

1562 2533

146 288

1206

1786

717 579

87 65

633 470

476 558 633 803 447 148 307 508 504 981 621

712 552

550 365 711 903 516 224 201 584 533

626 806

679 544

596 447 873 902 450 166 257 619 571

486 554 649 788 471 145 373 561 563

86 62

1318 1290

116 121

1168 1088

1213 1273

1163

1226 1188

114 116

882 622 151 335 886 781

681 159 343 872 762

56 23 32 82 77

579 132 459 817 718

619 184 330 800 707

649 159 324 816 725

56 23 32 88 85

xCys Met

Val

Ile

Leu Phe

1482

150

1259

1418

1004

1266

1399

1086

1001

149

713

928

59

589

378

620

445

702

680

944

492

460

667

59

Values are in mg/100 g product (powder or ready-to-feed liquid). The color code follows that on Appendix A and is based on the concentrations in the reconstituted final product according to the SMPR* * updated version proposed by the amino acid working group to be reviewed by the stakeholder panel during the August 2018 annual meeting.

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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