Amino-01 (July 2018)

Amino-01 (JULY 2018) 180124 AA SPIFAN SLV Appendices A-I

FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Laboratory Validation Report for Total Amino Acids by UHPLC-UV in Infant Formulas and Adult Nutritionals -– APPENDICES (A to I) Appendix I : example chromatograms for selected concentrations of the calibration curve and SPIFAN II SLV kit products. Low concentration standard (0.25 pmol/μL, 1 μL injected). Full (top) and zoomed (bottom) views

0.150

0.140

0.130

AMQ - 2.779

0.120

Deriv Peak - 16.957

0.110

0.100

0.090

0.080

AU

0.070

0.060

0.050

0.040

NVa - 18.597

0.030

0.020

21.855

0.010

NH3 - 3.291

21.658

Phe - 20.588 20.918

Leu - 20.299

Ile - 20.078

Lys - 17.267

21.359

Val - 18.293 18.418

Met - 18.020

17.070

Tyr - 17.632

Pro - 15.731 15.803

Ala - 14.584

Thr - 13.537

Glu - 12.037

Asp - 9.252

His - 3.661

Gly - 7.543

Tau - 4.825

Arg - 7.095

Ser - 6.143

1.670

0.000

Minutes 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50 21.00 21.50 22.00

0.0070

0.0065

0.0060

AMQ - 2.779

NVa - 18.597

0.0055

Deriv Peak - 16.957

0.0050

NH3 - 3.291

0.0045

0.0040

Phe - 20.588

0.0035

Leu - 20.299

Ile - 20.078

AU

Lys - 17.267

0.0030

20.918

Val - 18.293

0.0025

Met - 18.020

17.070

Tyr - 17.632

Pro - 15.731 15.803

0.0020

18.418

Ala - 14.584

0.0015

Thr - 13.537

Glu - 12.037

0.0010

Asp - 9.252

Gly - 7.543

His - 3.662

Tau - 4.825

Arg - 7.095

Ser - 6.143

0.0005

1.670

0.0000

Minutes 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50 19.00 19.50 20.00 20.50

Jaudzems, Guthrie, Lahrichi, and Fuerer – January 2018

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