4. AOACRIMicroMethods-2018Awards

138

816  B ird et al . : J ournal of AOAC I nternational V ol . 96, N o . 4, 2013

Table 2. Individual collaborator results for raw ground beef (25 g test portions) using traditional confirmation a High-level test portions Low-level test portions Uninoculated test portions Lab 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 VIDAS SPT (traditional confirmation) 1 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 2 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 3 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 4 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 5 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 6 + + + + + + + + + + + + + + + + + + + – b + + + + – – – – – – – – – – – – 7 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 8 c NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 9 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 10 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 11 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 12 c + + + + + + + + + + + + + + + + + + + + + + + + – – – c – – – – – – – – – 13 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 14 + + + + + + + + + + + + + + + + + + + + + + + + – – – – – – – – – – – – 15 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA USDA/FSIS-MLG 1 + + + + + + + + + + + + + – + – + + + – – + – + – – – – – – – – – – – – 2 + + + + + + + + + + + + + + + – – – + + – + – – – – – – – – – – – – – – 3 – + + + + + + + + + + + + + – + + + + + + – – – – – – – – – – – – – – – 4 + + + + + + + + + + + + – + + + + + – + + – + + – – – – – – – – – – – – 5 + + + + + + + + + + + + + + + + + + – + – + + + – – – – – – – – – – – – 6 + + + + + + – + + + + – – – – – + + + + – – + – – – – – – – – – – – – – 7 + + + + + + + + + + + + – + + – – – + – – + – + – – – – – – – – – – – – 8 c NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 9 + + + + + + + + + + + + + – – + + + + + – + – + – – – – – d – – – – – – – 10 – + + + + + + + + + – – + – – – – + + + + + – + – – – – – – – – – – – – 11 + + + + + + + + + + + + + + – + – + + – – + – – – – – – – – – – – – – – 12 c + + + + + + + + + + + + – + – + + + – – + – – – – – – – – – – – – – – – 13 + + + + + + + + + + + + – + + – + + – – + + – – – – – – – – – – – – – – 14 + + + + + + + + + + + + + + – + – + – + – – + + – – – – – – – – – – – – 15 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA a  + = Salmonella spp. were detected in samples; – = Salmonella spp. were not detected in sample; NA = laboratory did not participate in this matrix or results were not received. b  Sample was presumptive positive on VIDAS SPT, but confirmed negative indicating a false-positive result. c  Results were not used in statistical analysis due to laboratory error. d  Sample was confirmed negative on XLT4 and BGS, but confirmed positive on ASAP agar.

analysis. Laboratory 5 detected the presence of Salmonella  spp. in their reference method uninoculated control replicates; therefore, its results were not included for statistical analysis. Laboratory 2 detected the presence of Salmonella spp. in their VIDAS SPT replicates following the traditional confirmation procedure, and Laboratory 12 detected the presence of Salmonella spp. in the confirmation of VIDAS SPT replicates following the alternative confirmation procedure. Therefore, their results were not included in the statistical analysis for those categories. The MPN levels obtained for this test portion, with 95% confidence intervals, were 0.72 CFU/test portion

Raw Ground Beef (375 g Test Portions)

Raw ground beef test portions were inoculated at a low and a high level, and analyzed (Tables 4 and 5) for the detection of Salmonella spp. Uninoculated controls were included in each analysis. Fourteen laboratories participated in the analysis of this matrix; the results of 11 laboratories were included in the statistical analysis. Laboratory 8 reported that it was unable to confirm samples via serological testing and indicated that it did not conduct the alternative confirmation of the VIDAS SPT samples. Therefore, its results were not included in statistical 03/10/2019