4. AOACRIMicroMethods-2018Awards

41

B ird et al .: J ournal of AOAC I nternational V ol . 96, N o . 4, 2013  819

Table 5. Individual collaborator results for raw ground beef (375 g test portions) using alternative confirmation a High-level test portions Low-level test portions Uninoculated test portions Lab 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 VIDAS SPT (alternate confirmation) b 1 + + + + + + + + + + + + + + + + + – – – + – – – – – – – – – – – – – – – 2 + + + + + + + + + + – + + + + – – – + + – + – – – – – – – – – – – – – – 3 – + + + + + + + + + + + + + + + – – – – – + – – – – – – – – – – – – – – 4 + + + + + + + + + + + + + + – + – c – – – – – – NA – – – – – – – – – – – – 5 d + + + + + + + + + + + + + – + – – – – – + – + + – – – – – – – – – + – + 6 + + + + + + + + + + + + + – + + – + + – + – – – – – – – – – – – – – – – 7 + + + + + + + + + + + + + + + – – – + – – + – – – – – – – – – – – – – – 8 d NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 9 + + + + + + + + + + + + + + + – + + – + + – – – – – – – – – – – – – – – 10 + + + + + + + + + + + + + + + – + – – + + – – – – – – – – – – – – – – – 11 + + + + + + + + + + + + – – – – – – – – – – – – – – – – – – – – – – – – 12 d + + + + + + + + + – + + + + + – – – – – + + + + – – – – – – – – – – c – c – c 13 + + + + + + + + + + + + + + + – – + + + + – – – – – – – – – – – – – – – 14 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 15 + + + + + + + + + + + + + + + – – – – – + + – + – – – – – – – – – – – – USDA/FSIS-MLG 1 + + + + + + + + + + + + + + – – + + + – – – – – – – – – – – – – – – – – 2 + + + + + + + + + + + – – + + – – – – – – – – – – – – – – – – – – – – – 3 + + + + + + + + + + + + – – + – + + – – – – – – – – – – – – – – – – – – 4 + + + + + + + + + + + + + + – + + + + + + + – – – – – – – – – – – – – – 5 d + + + + + + + + + + + + – – + – – – – – – – – – – – + – – – – – – – – – 6 + + + + + + + + + + + + – + – + + + + – – – – – – – – – – – – – – – – – 7 + + + + + + + + + + + + + – – + + + + + – – – – – – – – – – – – – – – – 8 d NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 9 + + + + + + + + + + + + – – – – + + + + – – – – – – – – – – – – – – – – 10 + + + + + + + + + + + + + + – – – + – + – + – – – – – – – – – – – – – – 11 + + + + + + + + + + + + – – – + + + + + + – – – – – – – – – – – – – – – 12 + + + + + + + + + + + + – – + + + + – + – – – – – – – – – – – – – – – – 13 + + + + + + + + + + + + – + + – + – – – + + – – – – – – – – – – – – – – 14 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 15 + + + + + + + + + + + + + – – – – + – + + – – – – – – – – – – – – – – – a  += Salmonella spp. were detected in samples; –= Salmonella spp. were not detected in sample; NA = laboratory did not participate in this matrix or results were not received. b  Alternative confirmation = direct streak of the primary enrichment onto IBISA and ASAP. c  Sample was confirmed negative using the reference method agars XLT4 and BGS, but confirmed positive on ASAP and IBISA agar. d  Results were not used in statistical analysis due to laboratory error.

samples confirmed negative. For test portions analyzed by the USDA/FSIS-MLG method, all of the 132 high and 57 of 132 low inoculum test portions confirmed positive. For the uninoculated controls, none of the 132 test portions confirmed positive. For the low-level inoculum, dLPOD C values of 0.02 (–0.18, +0.22) were obtained between the USDA/FSIS-MLG method and the VIDAS SPT method. The confidence intervals obtained for dLPOD C indicated no significant difference between the two methods. dLPOD CP values of –0.01 (–0.15, +0.14) were obtained between presumptive and confirmed VIDAS SPT results. The confidence intervals obtained for dLPOD CP indicated no 03/10/2019

significant difference between the presumptive and confirmed results using either confirmation process. For the high-level inoculum, dLPOD C values of –0.02 (–0.05, +0.02) were obtained between the USDA/FSIS-MLG method and the VIDAS SPT method. The confidence intervals obtained for dLPOD C indicated no significant difference between the two methods. dLPOD CP values of 0.00 (–0.04, +0.04) were obtained between presumptive and confirmed VIDAS SPT results. The confidence intervals obtained for dLPOD CP indicated no significant difference between the presumptive and confirmed results. Detailed results of the POD statistical analysis are