4. AOACRIMicroMethods-2018Awards

B ird et al .: J ournal of aoaC i nternational V ol . 99, n o . 4, 2016 993

17

Table 4. Individual collaborator results for creamy peanut butter High level test portions

Low level test portions Uninoculated test portions 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 3M MDA 2 – Salmonella Laboratory a 1 + + + + + + + + + + + + + — — — — — — + + + + + — — — — — — — — — — — — 2 + + + + + + + + + + + + + + + + — — + — — + + + — — — — — — — — — — — — 3 b + + + + + + + + + + + + — — — — + c + — + + + + — — — — — — — — + — + c — — 4 + + + + + + + + + + + + + + — — — + — + — + + — — — — — — — — — — — — — 5 NA d NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 6 + + + + + + + + + + + + + + + — + + — + + — - e + — — — — — — — — — — — — 7 b + + + + + + + + + + + + + - e + — + - e + + + + — + — — — — — — — - e — — — — 8 + + + + + + + + + + + + + — — + — — + — + + + — — — — — — — — — — — — — 9 + + + + + + + + + + + + — + + + + — + + — + — — — — — — — — — — — — — — 10 + + + + + + + + + + + + — — + — — + + + — + + + — — — — — — — — — — — — 11 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 12 + + + + + + + + + + + + — + + + — — — — — — + + — — — — — — — — — — — — 13 + + + + + + + + + + + + + + — + — + + + + — + - e — — — — — — - e - e — — — — 14 + + + + + + + + + + + + + + + — — — + + + + — — — — — — — — — — — — — — 15 + + + + + + + + + + + + + + — — + + — + + — + + — — — — — — — — — — — — 16 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA FDA BAM Chapter 5 1 + + + + + + + + + + + + — — — — + + + — + + + — — — — — — — — — — — — — 2 + + + + + + + + + + + + — + — — + + — — — + + + — — — — — — — — — — — — 3 b + + + + + + + + + + + + + + — — + — — + — — — + — — — — — — — — — — — — 4 + + + + + + + + + + + + + — — + — — + — — — + + — — — — — — — — — — — — 5 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 6 + + + + + + + + + + + + + + — — — + — + + + + + — — — — — — — — — — — — 7 b + + + + + + + + + + + + — + + — + — — — + + + + — — — — — — — — — — — — 8 + + + + + + + + + + + + — — + + + — — — — — + + — — — — — — — — — — — — 9 + + + + + + + + + + + + + + + + + + + — — — + — — — — — — — — — — — — — 10 + + + + + + + + + + + + + + — — + — — — + — + — — — — — — — — — — — — — 11 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 12 + + + + + + + + + + + + — — + — + — + + + — + — — — — — — — — — — — — — 13 + + + + + + + + + + + + — — — — — + + — + + — + — — — — — — — — — — — — 14 + + + + + + + + + + + + + — + — + + + + + — + — — — — — — — — — — — — — 15 + + + + + + + + + + + + — + — — + + + + — + — — — — — — — — — — — — — — 16 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA a + = Salmonella spp. were detected in samples; - = Salmonella spp. were not detected in sample. b Results were not used in statistical analysis due to laboratory error. c Sample was presumptive negative on 3M MDA 2 – Salmonella but confirmed positive indicating a false negative result. d NA = Laboratory did not participate or submit data for the matrix. e Sample was presumptive positive on 3M MDA 2 – Salmonella but confirmed negative indicating a false positive result.

were submitted. All other laboratories submitted a full set of data for each method. The MPN levels obtained for this test portion were 0.67 CFU/test portion (95% confidence interval, 0.53–0.83) for the low inoculum level and 4.76 CFU/test portion (95% confidence interval, 3.41–6.65) for the high inoculum level. For the low inoculum level, 83 of 156 test portions (POD CP of 0.53) were reported as presumptive positive by the 3M MDA

Raw Ground Beef (73% Lean; 325 g Test Portions)

Raw ground beef test portions were inoculated at a low and high level and analyzed (Table 3) for the detection of Salmonella spp. Uninoculated controls were included in each analysis. Laboratories 7 and 10 did not participate in the evaluation of this matrix. Laboratory 5 received test portions but was unable to conduct the analysis and, therefore, no data

03/10/2019