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Table 2013.11A. Summary of results for the detection of Listeria monocytogenes in queso fresco (25 g) Method a VIDAS LMX w/OXA VIDAS LMX w/ALOA Inoculation level Uninoculated Low High Uninoculated Low High Candidate presumptive positive/total No. samples analyzed 0/156 77/156 156/156 0/156 77/156 156/156 Candidate presumptive POD (CP) 0.00 0.49 1.00 0.00 0.49 1.00 (0.00, 0.02) (0.41, 0.58) (0.98, 1.00) (0.00, 0.02) (0.41, 0.58) (0.98, 1.00) s r b 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.15) (0.46, 0.52) (0.00, 0.15) (0.00, 0.15) (0.46, 0.52) (0.00, 0.15) s L c 0.00 0.00 0.00 0.00 0.00 0.00 (0.00, 0.15) (0.00, 0.12) (0.00, 0.15) (0.00, 0.15) (0.00, 0.12) (0.00, 0.15) s R d 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.21) (0.47, 0.52) (0.00, 0.21) (0.00, 0.21) (0.47, 0.52) (0.00, 0.21) P value e 1.0000 0.9772 1.0000 1.0000 0.9772 1.0000 Candidate confirmed positive/total No. samples analyzed 0/156 75/156 156/156 0/156 75/156 156/156 Candidate confirmed POD (CC) 0.00 0.48 1.00 0.00 0.48 1.00 (0.00, 0.02) (0.40, 0.56) (0.98, 1.00) (0.00, 0.02) (0.40, 0.56) (0.98, 1.00) s r 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.15) (0.46, 0.52) (0.00, 0.15) (0.00, 0.15) (0.46, 0.52) (0.00, 0.15) s L 0.00 0.00 0.00 0.00 0.00 0.00 (0.00, 0.15) (0.00, 0.14) (0.00, 0.15) (0.00, 0.15) (0.00, 0.14) (0.00, 0.15) s R 0.00 0.51 0.00 0.00 0.51 0.00 (0.00, 0.21) (0.46, 0.52) (0.00, 0.21) (0.00, 0.21) (0.46, 0.52) (0.00, 0.21) P value 1.0000 0.8718 1.0000 1.0000 0.8718 1.0000 Positive reference samples/total No. samples analyzed 0/156 69/156 153/156 0/156 69/156 153/156 Reference POD 0.00 0.44 0.98 0.00 0.44 0.98 (0.00, 0.02) (0.36, 0.52) (0.94, 0.99) (0.00, 0.02) (0.36, 0.52) (0.94, 0.99) s r 0.00 0.51 0.13 0.00 0.51 0.13 (0.00, 0.15) (0.46, 0.52) (0.12, 0.15) (0.00, 0.15) (0.46, 0.52) (0.12, 0.15) s L 0.00 0.00 0.03 0.00 0.00 0.03 (0.00, 0.15) (0.00, 0.13) (0.00, 0.07) (0.00, 0.15) (0.00, 0.13) (0.00, 0.07) s R 0.00 0.51 0.14 0.00 0.51 0.14 (0.00, 0.21) (0.46, 0.52) (0.12, 0.16) (0.00, 0.21) (0.46, 0.52) (0.12, 0.16) P value 1.0000 0.9320 0.0877 1.0000 0.9320 0.0877 dLPOD (candidate vs reference) 0.00 0.04 0.02 0.00 0.04 0.02 (–0.02, 0.02) (–0.08, 0.15) (–0.01, 0.06) (–0.02, 0.02) (–0.08, 0.15) (–0.01, 0.06) dLPOD (candidate presumptive vs candidate confirmed) 0.00 0.01 0.00 0.00 0.01 0.00 (–0.02, 0.02) (–0.10, 0.13) (–0.02, 0.02) (–0.02, 0.02) (–0.10, 0.13) (–0.02, 0.02) a  Results include 95% confidence intervals. b  Repeatability standard deviation.

c  Among-laboratory standard deviation. d  Reproducibility standard deviation. e P value = Homogeneity test of laboratory PODs.

monocytogenes (LMX) assay kit from bioMérieux (595 Anglum Rd, Hazelwood, MO 63042-2330). ( a )  VIDAS or miniVIDAS automated immunoassay System. ( b )  LMX reagent strips.— Sixty polypropylene strips of 03/10/2019

10 wells, each strip covered with a foil seal and label. The 10 wells contain the reagents in Table 2013.11C . ( c )  SPR.— Sixty SPRs coated with proteins specific for Listeria receptors . ( d )  Standard.— One vial (1×6 mL). Ready-to-use. Contains