4. AOACRIMicroMethods-2018Awards

B ird et al .: J ournal of aoaC i nternational V ol . 99, n o . 4, 2016 995

19

Figure 2. dPOD values of candidate method versus reference method for 325 g raw ground beef (73%).

no statistically significant difference between the presumptive and confirmed results. For the high inoculum level, 132 of 132 test portions (POD CP of 1.00) were reported as presumptive positive by the 3M MDA 2 – Salmonella method, with 132 test portions (POD CC of 1.00) confirming positive. For samples that produced presumptive positive results on the 3M MDA 2 – Salmonella method, 132 samples confirmed positive (POD C of 1.00). For test portions evaluated by the FDA BAM reference method, 132 of 132 test portions produced positive results. A dLPOD C value of 0.00 (95% confidence interval, −0.03 to 0.03) was obtained between the candidate and reference method, indicating no statistically significant difference between the two methods. A dLPOD CP value of 0.00 (95% confidence interval, −0.03 to 0.03) was obtained between presumptive and confirmed results, indicating no significant difference found between the presumptive and confirmed results. For the uninoculated controls, 2 of 132 samples (POD CP of 0.01) produced a presumptive positive result by the 3M MDA 2 – Salmonella method, with 0 test portions (POD CC of 0.00) confirming positive. For samples that produced presumptive positive results on the 3M MDA 2 – Salmonella method, 0 samples confirmed positive (POD C of 0.00). For test portions evaluated by the FDA BAM reference method, 0 of 132 test portions produced positive results. A dLPOD C value of 0.00 (95% confidence interval, −0.03 to 0.03) was obtained between the candidate and reference method, indicating no statistical significant difference between the two methods. A dLPOD CP value of 0.01 (95% confidence interval, −0.01 to 0.05) was obtained between presumptive and confirmed results, indicating no significant difference between the presumptive and confirmed results. Detailed results of the POD statistical analysis are presented in Table 2016.01D and Figures 3 and 4.

Creamy Peanut Butter (25 g Test Portions)

Creamy peanut butter test portions were inoculated at a low and high inoculum level and were analyzed (Table 4) for the detection of Salmonella spp. Uninoculated controls were included in each analysis. Laboratory 16 did not participate in the evaluation of this matrix. Laboratories 5 and 11 received test portions but were unable to conduct the analysis and therefore no data were submitted. Laboratory 7 reported deviations from the testing protocol in the heat lysis step (incorrect temperature used). Laboratory 3 reported contamination of its uninoculated control samples with the inoculating strain. Due to this issue, the data submitted from Laboratories 3 and 7 were not used in the statistical analysis. All other laboratories submitted complete data for each method. The MPN levels obtained for this test portion were 0.70 CFU/test portion (95% confidence interval, 0.55–0.89) for the low level and 5.35 CFU/test portion (95% confidence interval, 3.46–8.24) for the high level. For the low inoculum level, 78 of 132 test portions (POD CP of 0.59) were reported as presumptive positive by the 3M MDA 2 – Salmonella method, with 76 test portions (POD CC of 0.58) confirming positive. For samples that produced presumptive positive results on the 3M MDA 2 – Salmonella method, 76 samples confirmed positive (POD C of 0.58). For test portions evaluated by the FDA BAM reference method, 68 of 132 test portions produced positive results. A dLPOD C value of 0.06 (95% confidence interval, −0.06 to 0.18) was obtained between the candidate and reference method, indicating no statistically significant difference between the two methods. A dLPOD CP value of 0.02 (95% confidence interval, −0.11 to 0.14) was obtained between presumptive and confirmed results, indicating

03/10/2019