4. AOACRIMicroMethods-2018Awards
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B ird et al .: J ournal of AOAC I nternational V ol . 100, N o . 2, 2017 461
Table 2016.08D. Comparative results for the detection of L. monocytogenes at three inoculum levels in 25 g raw chicken breast fillet test portions by the 3M MDA 2 – L. monocytogenes method vs the USDA/FSIS MLG reference method in a collaborative study
Candidate presumptive
Candidate confirmed
Candidate result
Reference method
Candidate vs reference
n a
x b
Statistic Matrix Laboratory
POD CP n x
POD CC
POD C n x
POD R
dLPOD C
dLPOD CP
n x
Uninoculated control
Raw chicken breast fillet
1 2 3 4 5 6 7 8 9
12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 0 12 0 0.00 12 2
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.17 0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23 0.00 0.00
12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0
0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 12 0 0.00 0.00 132 0 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 NA
0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00
10 c
−0.17
11 NA d
NA NA NA NA
NA NA NA NA NA NA NA
NA
12 12 0 0.00 12 0 13 12 0 0.00 12 0
12 0 12 0
0.00 0.00 0.00
Estimate
All
132 0 0.00 132 0
0.00 132 0
LCL e UCL f
0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23
0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23
0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23
−0.03
−0.03
0.03
0.03
s r
LCL UCL
s L
LCL UCL
s R
UCL LCL
1.0000
1.0000
1.0000
1.0000
P T
Low inoculum level
1
12 7 0.58 12 7
0.58
12 7
0.58 12 7 0.58
0.00
0.00
Raw chicken breast fillet
2 3 4 5 6 7 8 9
12 9 0.75 12 9 12 10 0.83 12 10 12 9 0.75 12 9 12 8 0.66 12 8 12 9 0.75 12 9 12 7 0.58 12 6 12 7 0.58 12 7 12 8 0.66 12 9 12 6 0.50 12 8
0.75 0.83 0.75 0.66 0.75 0.50 0.58 0.75 0.66
12 9
0.75 12 7 0.58
0.17 0.41 0.42 0.22 0.15 0.00 0.00 0.08 0.08
0.00 0.00 0.00 0.00 0.08 0.16 0.00
12 10 0.83 12 5 0.42
12 9 12 8 12 9 12 6 12 7 12 8 12 6
0.75 12 4 0.33 0.66 12 5 0.42 0.75 12 6 0.50 0.50 12 6 0.50 0.58 12 7 0.58 0.66 12 7 0.58 0.50 12 5 0.42
−0.08 −0.16
10 c
11 NA NA NA NA NA 12 12 7 0.58 12 7 13 12 5 0.42 12 5
NA NA NA NA NA NA NA
NA
NA
0.58 0.42
12 7 12 5
0.58 12 4 0.42 0.42 12 6 0.50
0.16
0.00 0.00
−0.08
MPN, CFU/ test portion
Estimate 0.66
All
132 86 0.65 132 86
0.65 132 85 0.64 132 64 0.48
0.16 0.04 0.28
0.00
LCL UCL
0.51 0.83
0.57 0.73
0.57 0.73
0.56 0.73
0.40 0.57
−0.12
0.12
03/10/2019
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