SPDS EXPERT REVIEW PANEL

2018 SMPR ERPs - METHOD REVIEW FORM

Submission ID

4108790659463308184

Submission Date

2018-08-23 19:17:45

AOAC Expert Review Panel: Method Review

Reviewer Information

Name

Garrett Zielinski

E-mail

garrettzielinski@eurofinsus.com

Organization

Eurofins Food Integrity & Innovation

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Determination of Active Vitamin B12 (cobalamin) in dietary supplements and ingredients by reversed-phased liquid chromatography: Single Laboratory Validation

AOAC Candidate Method Number (e.g. ALN-01)

B12-001

Applicable SMPR

2016.017

I. Summary of the Method

A. Summary of the Method

Sample is extracted with aqueous solvent and the content of four active forms of cobalamin (hydroxo, adenosyl, cyano, and methyl) are determined via RP-HPLC with UV detection using an external standard curve. Method is applicable for tablets, capsules, and powders.

II. Review of the Method Only: