PCR Supplement

PCR Specials Phenix Research Products

Summer 2016

A Revolution in qPCR MyGo Mini $7,295 See Page 4

PCR & Genomics

Inside PCR Strips and Plates Sealing Film & Mats PCRWork Stations Thermal Cyclers Agarose Gel Red DNA Dye Heat Sealers

AzuraQuant TM cDNA Synthesis Kit TheAzuraQuant™cDNASynthesisKitprovidesarapidandsensitivemethod forgeneratinghighquality, qPCR-readyfirst-strandcDNAforuse indownstreamreal-timePCR.TheAzuraQuant™cDNASynthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5’ and 3’ mRNA ends. Win an $8,000 qPCR Machine

PCR Equipment

Next Generation cDNA & qPCR Kits

Enter Our PCR Contest The kit was developed to provide consistent linearity across a wide range of input RNA, revealing accurate relative cDNA representation, regardless of gene abundance. HighTherm™ reverse transcriptase which provides exceptional thermostability is blended with a potent RNase inhibitor to ensure high integrity of Total RNAstartingmaterial. The AzuraQuant™ cDNA Synthesis Kit is supplied with a high-performance 5x buffer system including an optimized ratio of random hexamer primers and anchored oligo (dT) primers which delivers unbiased, efficient and sensitive cDNA synthesis. The AzuraQuant™ cDNA Synthesis Kit delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility and data accuracy. TheAzuraQuan tTM cDNASynthesisKitprovidesand ideal chemistry. for success with limited starting material, suchas tissuebiopsies. See Back of Book

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See Pages 6 & 7

Fig1.BroadDynamicRangewithAzuraQuant TM cDNASynthesisKit. • A10 foldserialdilutionofmouse totalRNAwasused forsevencDNA synthesis reactions (withstartingmaterial ranging from2µg to2pg TotalRNA).Subsequently,eachcDNAsynthesis reactionwasusedas a template forqPCR inwhicha70bp fragmentwasamplified (mouse RN18Sgene).

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