SPDS Set 1 ERP Book
CHON-001
Question
Response
General
AOAC Method Number:
CHON-001
Selected Adulterants in Dietary Ingredients and Dietary Supplements Containing Chodroitin Sulfate
Method Title:
Method Author(s):
United States Pharmaopeia
Reviewer Name:
Jana Hildreth
Cellulose acetate membranes e;ectophoresis with Toluidine Blue staining based on the characteristic migration pattern of chondroitin sulfate on cellulose acetate membranes when subject to a voltaic current, producing visible very well defined bands after staining the developed membranes with a cationic dye (Toluidine Blue) . Screening method for selected adulterants in dietary ingredients claiming to contain chondroitin sulfate: Sodium hexametaphosphate Sodium alginate Propylene glycol alginate sulfate sodium Over-sulfated polysaccharides This method has a proven track record of finding adulterants in chondroitin sulfate raw material and has been in pharmacopieas worldwide. This is an extremely straight forward method that does not require much training. The cost of running this method is very cheap. It has proven to find new adulterants that more sophisticated methods missed. The cost of the equipment is under $2000 and training is minimal. Currently only a couple of labs have the set up to run this method in the US. Supporting Data
Summary of Method
Method Scope / Applicability:
General Comments About the Method:
Method Clarity
Pros/Strengths
Cons/Weaknesses
General Comments
I feel this is a great screening tool for incoming chodroitin
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