SPDS Set 1 ERP Book
CHON-003
Supporting Data
General Comments
Not given
Method Optimization
No Performance Characteristics
Analytical Range:
Not Given
LOQ:
Not Given
Accuracy/Recovery
102.5-103.9 %
Precision (RSDr)
2.38 %
Reproducibility (RSDR)
1.74 %
System Suitability
May be suitable for routine analysis Safety Review
Is the method safe to use as written? Are there any safety concerns with any component, instrument, reagent or instruction of the method? If yes, please clearly state the concerns. Does the method include all necessary safety precautions and/or warning statements? Yes or No. If No, please suggest potential precautions or warnings that can be added to the method for users or recommend safer option(s) if warranted.
Yes
No
Yes
Recommendations
Several spectrophotometric assays for sulfated GAG quantification in biological samples based on the use of DMMB have been reported. All of these methods rely on the absorption at about 525 nm of the GAGs-DMMB complex formed after addition of the dye to biological samples. In all reported methods, this complex is formed in a reaction mixture containing a large excess of the dye, which absorbs in wavelengths that might interfere with the detection of the GAG-dye complex. Among other drawbacks of DMMB classical methods, the presence of a precipitate of the GAG- dye complex has been reported to render quantification
Final Recommendation:
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