SPADA Working Groups ~ April Meeting Book
C. burnetii phylogenetic organization
SNP and VNTR based trees for 25 worldwide isolates of Coxiella burnetii. Geographical distribution shown below. Pearson, Keim et al. ASM2005
Glazunova et al. EID 2005
C. burnetii – Existing PCR assays • Purpose: Detect C. burnetii DNA in clinical and environmental samples – Environmental samples, milk, soil, animal Tissues, air sampling – Human blood samples • Most common targets: IS1111, mulit‐copy IS element – Range in copy number among isolates (~20) Com‐1: encodes outer membrane (DsbA/C) protein – Highly conserved among isolates • qPCR method – Brennan et al (IAI 2003) – Sensitivity approaches one genome equivalent
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