SPADA Working Groups ~ April Meeting Book

AOAC Stakeholder Panel on Agent Detection Assays: VEE Working Group Teleconference

Meeting Minutes Thursday, April 7, 2015; 10:00 a.m. – 11:00 a.m. EST

Attendees

Panel Members (Present during all or part of the meeting) : Eileen Ostlund, USDA (Chair) Jeff Ballin, ECBC Linda Beck, Naval Surface Warfare Center Ryan Cahall, Censeo Insight Joan Gebhardt, Naval Medical Research Center Tom Phillips, MD Department of Agriculture Ann Powers, CDC Jon Rayner, SRI Scott Weaver, U. Texas Medical Branch

AOAC Staff (Present during all or part of the meeting) :

Scott Coates Christopher Dent Krystyna McIver Deborah McKenzie

Meeting Minutes

I.

Welcome and Introductions · Roll call was taken and all were introduced.

II.

SMPR Development

· Ostlund explained that although the SMPR has been limited to Venezuelan Equine Encephalitis the next question is which VEEs? Ostlund distributed a spreadsheet 1 containing more information on the various types of VEE. The table was reviewed and there were no objections to including VEE-IAB, VEE-IC, and VEE-IE. · The group then discussed the inclusion of Mucambo. Beck stated that Mucambo may be so unique that it would be a separate test altogether. Should Mucambo be included in this SMPR? Cahall said that he will take this question back to the DUSA-TE team, who is funding this project, to get clarification on if this is something DoD wants included; however the group came to consensus that for this SMPR Mucambo would not be included. · Coates recommended the other strains on the table be included as part of the Exclusivity Panel, with the expectation that this SMPr will be specific to VEE-IAB, VEE-IC, and VEE-IE. The group agreed. · The group also agreed on setting the maximum time to result at four hours. · Returning to the table in Section 7, the group agreed to an AMIL of 50,000 genome copies per mL. The POI remained at 0.95. · Environmental testing will be done from buffer. · For exclusivity, the question remains whether or not to include environmental panel organisms. DNA will be pooled up to 10 at a time for environmental organisms.

1 Attachment 1: VEE Spreadsheet