SPADA Working Groups ~ April Meeting Book

Draft, Do Not Distribute

AOAC SMPR 2015.XXX; Version 4, April 22, 2015

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Method Name:

Identification of Venezuelan Equine Encephalitis Virus (VEEV)

AOAC Stakeholder Panel on Agent Detection Assays

Approved Body:

1. Intended Use :

Laboratory or field use by trained operators.

2. Applicability : 9 10 11 3. Analytical Technique : Molecular methods of detecting target-specific viral component(s).

Identification of VEEV in liquid samples from aerosol collectors. The preferential method would be a field-deployable assay.

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4. Definitions :

Acceptable Minimum Identification Level (AMIL) 16 The predetermined minimum level of an analyte, as specified by an expert committee which must be detected and identified by the candidate 17 method with a specified probability of identification (POI).

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Exclusivity

21 Study involving pure non-target strains and species, which are potentially cross-reactive, that shall not be detected or identified by the test method.

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Inclusivity

24 Study involving pure target strains or species that shall be detected and identified by the alternative method.

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Maximum Time-To-Assay Result

Maximum time to complete an analysis starting from the test portion preparation to assay result.

Probability of Identification (POI) 30 The proportion of positive analytical outcomes for an identification method for a given matrix at a given analyte level or concentration.

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1 Draft EEVs SMPR V4