Biomek iSeries
TABLE OF CONTENTS
TABLE OF CONTENTS
March 2018
Brochures & Flyers • i-Series Automated Workstations Brochure........................................................................................................................... 1 • Biomek Wide Bore Tips: Ensure Biological Integrity of Samples.............................................................................. 13 • Biomek Pipette Tips: The Most Critical Point of Contact.............................................................................................. 15 • Biomek Tips and Labware Brochure....................................................................................................................................... 17 • Biomek i-Series Pipetting Performance Capabilities..................................................................................................... 37 • Biomek-Automated Genomic Solutions: Take your next step with confidence................................................ 39 • Biomek-Automated NGS Library Construction Methods............................................................................................ 47 • i-Series Drug Discovery and Basic research Brochure ................................................................................................. 49 • Integrated Solutions Device List.............................................................................................................................................. 57 • Genomics Products Portfolio Overview................................................................................................................................ 62 Application & Technical Notes • Simple, yet effective – automating Bradford assays ..................................................................................................... 65 • Automated Cell Plating and Growth Assays...................................................................................................................... 67 • Flexible ELISA automation with the Biomek i5 Workstation..................................................................................... 69 • Efficient Factorial Optimization of Transfection Conditions....................................................................................... 71 • Fully-Automated Cellular Analysis by Flow Cytometry................................................................................................ 74 • Biomek Automated Genomic Sample Prep Accelerates Research......................................................................... 76 • Biomek i-Series Automated Beckman Coulter AMPure XP PCR Purification System.................................... 81 • Biomek i-Series Automated FormaPure DNA Extraction kit for FFPE Samples.............................................. 84 • Biomek i-Series Automated Illumina TruSeq DNA PCR-Free.................................................................................... 87 • Biomek i-Series Automated Illumina TruSeq Exome Library Prep Kit................................................................... 90 • Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips.................................................................................................................... 94 • Consistent Cell Maintenance and Plating through Automation .............................................................................. 98 • Biomek Automated TruSight Tumor 170 Assay - Illumina Qualified........................................................................101 WorkFlow Overview • Continuous Cell Culture: Automating Cell-Based Processes................................................................................... 105 • High Throughput Screening: Make an impact by removing the barriers........................................................... 107 • Transfection: Efficient, high-quality results with automated transfection methods..................................... 109 • Cell Culture: Automating Cell Maintenance......................................................................................................................... 111 • Cell-Based Assays: One platform to meet your evolving assay needs................................................................. 113 • Cell Line Development: Boost Your Labs’ Efficiency..................................................................................................... 115
Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
For Beckman Coulter’s worldwide office locations and phone numbers, please visit beckmancoulter.com/contact
© 2018 Beckman Coulter, Inc.
DESIGNED FOR YOUR EVOLVING WORKFLOW PRIORITIES Biomek i-Series Automated Workstations
ACCELERATED WORKFLOWS . Faster Discovery.
YOU TALKED. WE LISTENED . Your priorities continue to evolve; ours never change. Because our priority is you. Your input and our experience led to the engineering of a liquid handling platform that enables you to get the trusted, reliable results you need from your application workflows. From your input, some recurring themes appeared. And from those themes emerged a common foundation—for hardware, software, accessories, consumables and support—that’s built-on: • Simplification, so you can focus more on science and less on managing your tools • Efficiency, to help you optimize productivity, increase walk-away time and learn more—faster • Adaptability, so the technology you invest in can grow with you, not grow obsolete • Reliability and support, to help maximize instrument uptime so your research is always moving forward On that customer-driven foundation, we’re building the future of laboratory automation.
The benefits of an automated workstation from Beckman Coulter Life Sciences come from more than just an instrument. They’re realized thanks to synergy between:
• Hardware • Software • Accessories • Consumables • Service and support
i - Series
ACCELERATED WORKFLOWS . Faster Discovery.
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ADVANCING SCIENCE BY ACCELERATING A WIDE RANGE OF APPLICATIONS Automation drives acceleration. This is true for pharma, biotech, academia and government-sponsored research—virtually any lab focused on genomics, cell biology, proteomics, drug discovery, forensics and related research areas. A sampling of drug discovery and basic research application areas that can benefit from Biomek automation:
• Cell-based assays • High-throughput screening
• Continuous cell culture • Biologic bioanalysis • Proteomics, lipidomics, metabolomics • Synthetic biology • 3D cell models • Compound handling • Nucleic acid sample prep °° DNA/RNA isolation °° DNA/RNA clean-up °° DNA size selection °° Plasmid prep • Sanger sequencing °° Big dye clean-up • qPCR/PCR setup
• High-content screening • Cell line development
• Cell culture • Transfection
A sampling of genomics application areas that can benefit from Biomek automation:
• Next-generation sequencing sample prep °° Whole genome sequencing °° Transcriptome sequencing °° Target/exome capture °° Amplicon sequencing °° Cancer panels °° HLA typing • Microarray sample prep °° Genotyping °° Gene expression
Many fields of research continue to accelerate at a rapid pace. Our commitment is to provide the innovative technology you need to maintain that pace—by simplifying your workflows, improving efficiency, increasing walk-away time and decreasing downtime.
The most recent example of this commitment: Biomek i-Series Automated Workstations1.
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EXPLORE THE BIOMEK i-SERIES AUTOMATED Representing the best of what has made Biomek workstations an industry leader—combined with enhancements suggested by customers around the globe—Biomek i-Series Automated Workstations have been designed to optimize dependability and walk-away time in mid- to high-throughput labs.
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Simplicity so you can focus more on science Bright, multiple color- and pattern-coded status light bar alerts you to the instrument’s current mode, even from across the room. Light Curtain provides key safety feature during operation and method development. Internal LED light illuminates the instrument deck for easy access and monitoring of your workspace status. 1 2 3
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Efficiency to help deliver higher productivity
Rotating gripper with unique offset finger design optimizes access to high-density decks, enabling more efficient workflows. Linear motion control increases positional accuracy for pipetting access to high-density labware. Large-volume, 1 mL multichannel pipetting head expedites sample transfers and enables more efficient mixing steps.
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Biomek i7 Automated Workstation
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ACCELERATED WORKFLOWS . Faster Discovery.
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WORKSTATIONS
BIOMEK i-SERIES WORKSTATIONS ENABLE “4D INTEGRATION” With access from 4 directions—
right, left, back and below—i- Series workstations make it easy to integrate a growing menu of devices, components and accessories. Your Beckman Coulter team can help you with standard integrations.
Adaptability to extend scale and reach Grid-based deck with simple accessory installation enables quick workflow changes. Spacious, open-platform design enables access from all sides to enable integration of adjacent-to-deck and off-deck processing elements (e.g., analytical devices, external storage/incubation units, and labware feeders). 7 8 Trusted reliability and support to reduce downtime Safeguard sample and reagent integrity from air particulates with enclosed versions of the Biomek i-Series Automated Workstations. Vertical sliding door on enclosure provides front access without aisle obstruction. Onboard cameras enable live broadcast and on-error video capture to expedite response time and system diagnosis. Span-8 pipetting with independent probe calibration for volume transfers from 0.5 to 5,000 μL. 9 10 11 12
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YOUR APPLICATIONS
ACCESSORIES TO ENABLE
Build a system to suit your needs. Choose the accessories you need to maximize the efficiency and reliability delivered by a Biomek i-Series Automated Workstation.
Multichannel heads In addition to Span-8 pipetting, three multichannel head options are available:
RANGE
MC-384, 60 μL
0.5 μL - 60 μL
MC-96, 300 μL
1 μL - 300 μL
MC-96, 1,200 μL
5 μL - 1,200 μL
For more information on compatible tips, request a copy of Beckman Coulter’s Biomek Tips and Labware catalog.
Automated Labware Positioners (ALPs)
Designed to fit seamlessly onto the deck of any Biomek workstation, ALPs from Beckman Coulter Life Sciences perform application-specific tasks, such as: • Tip washing • Shaking • Heating/cooling • Reagent reservoirs • And a host of other options depending on your application
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ACCELERATED WORKFLOWS . Faster Discovery.
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Device integrations and customization services To further enhance the capabilities of your Biomek i-Series Automated Workstation, you can choose from a list of device integrations or ask for our customization services. Your support team will include experts who’ve integrated hundreds of third-party devices to meet a large variety of workflow needs.
Additional components • Fly-by barcode reader for positive sample ID, process and tracking of microplates • Labware feeders • Instrument carts (pictured) and tables • And a host of others depending on your specific needs
Biomek i5 Automated Workstation
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INDUSTRY-LEADING BIOMEK SOFTWARE FOR i-SERIES THAT DELIVERS WORKFLOW INTELLIGENCE The latest version of customer-acclaimed Biomek software, Biomek i-Series software maximizes ROI by giving you unprecedented control over your liquid handler.
Method authoring • Sets the standard for method authoring with a point-and-click interface; no advanced software expertise required. • Saves time and consumables with Biomek’s visual editor, which validates your method in real time as you create it, and Biomek’s 3D simulator, which demonstrates exactly how your method will execute. • Provides full control over movement of the tip in the well to match even the most complex manual pipettingmotions. Ease of operation • Improves accuracy and reduces errors by giving operators step-by-step guidance for placing labware on the deck. • Makes it easy for lab technicians to launch/monitor methods by providing a simple point-and-click user interface. • Lets you “lock down” the instrument and protect validated methods from being altered inadvertently by operators. • Supports regulated laboratories and multi-user environments by controlling access using electronic signatures. • Enables remote instrument monitoring using any device with a Google Chrome browser. Data management • Captures the data you need to validate your processes and help ensure reproducible results. • Integrates with LIMS systems to import work orders and export data. • Seamlessly transfers data between methods so you can easily generate run, labware and sample reports at any time. • Data-driven methods select appropriate actions during execution based on sample data generated in real time.
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ACCELERATED WORKFLOWS . Faster Discovery.
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Additional software solutions include:
Simplify data management with DART Automation systems generate large quantities of data to manage. DART simplifies workflow data by transforming it into powerful information to inform your research decisions.
Streamline workflows with SAMI EX Automate full workflows with a process control supporting a wide variety of applications. Use the power of a planning scheduler and sample data drill-down to manage your assay.
Easily automate your most complex workflows with SAMI Process Management
This calendar-format tool enables the addition, monitoring and planning of SAMI EX methods and other events as part of user-defined processes.
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BIOMEK PIPETTE TIPS: THE MOST CRITICAL POINT OF CONTACT
All Biomek workstations are optimized to perform as complete systems. That’s why only Biomek pipette tips from Beckman Coulter Life Sciences are validated and approved for use with our workstations. Biomek pipette tips, racks and rack covers are molded from premium-grade, virgin polypropylene to provide chemical resistance during sample preparation, and to ensure the highest performance and quality control for your most exacting applications. To make setup, method writing, and operation easy and efficient, Biomek software already includes point-and-click options for Biomek tip definitions, pipetting techniques and templates, and color-coded Biomek tip racks.
Only Biomek pipette tips are validated and approved for use with Biomek workstations.
Biomek pipette tips from Beckman Coulter are certified to be: • RNase- and DNase-free Ensures high-quality nucleic acid purification and reliable nucleic acid testing results. • DNA-free (human & mouse)/PCR inhibition Confirms the absence of both human and mouse DNA contaminants that result in erroneous results and interfere with the PCR. • Endotoxin-free Addresses endotoxin-sensitive applications, such as cell transformations and preparative protocols that use extracted biological material for in vivo experimentation. • Trace metal-free
Testing of the base, polypropylene tip resin reduces interferences due to chelation and denaturation.
For more information on Biomek tips and accessories, request a copy of Beckman Coulter’s Biomek Tips and Labware catalog or visit beckman.com .
i - Series 10 | ACCELERATED WORKFLOWS . Faster Discovery.
WORLD-CLASS TRAINING, SERVICE AND SUPPORT ARE INSTRUMENTAL TO SUCCESS
From online classes or instructor-led training, to on-site application support – we’re committed to doing everything possible to ensure that you can operate your Biomek workstation with confidence. In addition, when you choose a Biomek i-Series Automated Workstation from Beckman Coulter Life Sciences, you can expect support and maintenance from an organization that’s rated higher for technical support than any major competitor. Further building on our commitment to world-class service, the PROService Remote Monitoring System is now available to help maximize system uptime by shortening service calls and expediting repair times.
Instructor-led training or on-line classes help ensure that you can operate your i-Series workstation with confidence.
Our customer support center directs a team of engineers with extensive product knowledge who can trouble- shoot issues quickly and efficiently.
To help maximize instrument uptime, the PROService Remote Monitoring System can use built-in cameras to show our support team a secure view of your i-Series workstation.
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SYSTEM SPECIFICATIONS FOR BIOMEK i-SERIES AUTOMATED WORKSTATIONS
ITEM
WITHOUT ENCLOSURE
WITH ENCLOSURE
Width: 112 cm (44”) Depth: 81 cm (32”) Height: 104 cm (41”) Width: 170 cm (67”) Depth: 81 cm (32”) Height: 104 cm (41”)
Width: 112 cm (44”) Depth: 81 cm (32”) Height: 112 cm (44”) Width: 170 cm (67”) Depth: 81 cm (32”) Height: 112 cm (44”)
Dimensions for Biomek i5 Base Unit
Dimensions for Biomek i7 Base Unit
Maximum Height w/Door Open
N/A
147 cm (58”)
Weight: Biomek i5 Base Unit
Multichannel
155 kg (341 lbs)
181 kg (399 lbs)
Span-8
146 kg (322 lbs)
172 kg (379 lbs)
Weight: Biomek i7 Base Unit
Multichannel
199 kg (439 lbs)
234 kg (516 lbs)
Dual Multichannel
234 kg (516 lbs)
269 kg (593 lbs)
Span-8
190 kg (419 lbs)
225 kg (496 lbs)
Hybrid (Multichannel + Span-8)
225 kg (496 lbs)
260 kg (573 lbs)
Introducing the i-Series to our family of Biomek Automated Workstations is just the beginning of our journey together. Biomek hardware, software and our growing applications portfolio will continue to evolve with your priorities in mind. Visit biomek.beckman.com or speak with your Beckman Coulter representative to learn more.
1 Currently in development © 2017 Beckman Coulter, Inc. All rights reserved.
Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com AAG-2215SB01.17
Biomek Wide Bore Pipette Tips minimize the inadvertent harm, which can be applied to native biological sample matrices during sample preparation. Loss of biological sample integrity can occur when pipetting fragile cell suspensions, hybridomas, embryoid bodies, algal coagulates, protein aggregates, and genomic materials due to the pipette tips’ small internal orifice diameter and the speed of aspiration and dispense during pipetting. Biomek Wide Bore Tips have been designed to address such sample preparation challenges with a pipetting orifice twice the size of standard pipette tips.¹ Ensure Biological Integrity of Samples BIOMEK WIDE BORE PIPETTE TIPS
Ensure biological integrity of samples Reduce flow-induced shear strain on native biological sample matrices with wide bore tips. Minimize DNA shearing Avoid the risk of inadvertent DNA shearing, while preparing samples during nucleic acid extraction and purification protocols. Improve sample throughput Increase throughput when pipetting viscous reagent solutions, such as glycerol stocks, or bead mixtures. Minimize cell lysis Prevent inadvertent cell lysis when pipetting fragile cell suspensions, hybridomas, or embryoid bodies. Reduce aggregate disruptions Maintain the natural state of protein aggregates in therapeutic formulation studies and algal coagulated in biomass studies. Prevent tip clogging Avoid obstructions due to heavy particulate or fibrous sample matrices as encountered in biomass studies and agricultural research.
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Comparison of Standard vs. Wide Bore Orifice Pipette Tip Diameters
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For the best in system performance, use only Beckman Coulter validated tips on Biomek workstations.
1.4 mm * (Wide Bore)
0.6 mm * (Standard)
* Enlarged for illustrative purposes only.
Improved Pipetting Efficiency The capability to transfer a full 1 mL of samples or reagents in a single pipetting step provides greater efficiency and timesavings during sample preparation. The 1 mL tips are commonly used for large volume transfers applied to nucleic acid extraction and purification protocols, “seed and feed” cellular applications, and general reagent pipetting. The overall length of the tips allows access to the full contents of long, narrow tubes, such as 12 x 75 mm and 16 x 100 mm test tubes, 15 and 50 mL centrifugation tubes, and 1 and 2 mL deep-well plates. For added confidence in Biomek pipetting performance during method development, the non-conductive, Wide
Bore Tips offer the advantage of being clear (versus black, conductive) to allow for visual inspection of aspirated volumes. With the clear tips, researchers are able to see how much volume they have aspirated into the tips, and the uniformity of aspirated volume across tips. In addition, Biomek Wide Bore Tips reduce sample preparation times by allowing faster pipetting speeds of viscous reagent solutions, such as glycerol stocks, or bead mixtures. The larger diameter bore also minimizes tip clogging when pipetting particulate- laden or fibrous sample matrices as those found in biomass studies and agricultural research.
Biomek Wide Bore Pipette Tips Ordering Information
STERILE (PRE-STERILE)
STERILEWITH FILTER (BARRIER)
NON-STERILE
PACKAGING
For use with Biomek 4000, and Biomek NX p and FX p Span-8 Automated Workstations.*
96 tips/tip box, 10 tip boxes/case
P250
B01101
B01102
B01103
96 tips/tip box, 5 tip boxes/case
P1000
B01112
B01113
B01114
For use with Biomek i-Series Automated Workstations.
96 tips/tip box, 10 tip boxes/case
230/190 μL
B85926
B85929
B85936
96 tips/tip box, 5 tip boxes/case
1070/1025 μL
B85971
B85975
B85981
Environmental Awareness & Sustainability Biomek tips, racks, and rack covers are molded from polypropylene resin to provide chemical resistance during sample preparation. The Society of the Plastics Industry (SPI) identifies polypropylene with an internationally recognized resin identification code number of 5 for recycling purposes.
Bio-certifications Biomek tips are certified to be free of RNase, DNase, DNA (human and mouse) / PCR inhibition, endotoxins, and trace metals.
For additional information, request: Technical Information Bulletin, IB-17295A, Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate; Biomek Wide Bore vs. Standard Pipette Tips , Robert D. Lund, Beckman Coulter Life Sciences, Indianapolis, IN. ¹
Biomek tip packaging is comprised of 100% recyclable corrugated fiberboard.
*i-Series in development. © 2016 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com AAG-2182FLY12.16
Biomek pipette tips from Beckman Coulter are the only pipette tips validated and approved for use with Biomek workstations. Our tips work seamlessly with Biomek hardware and are fully integrated into our exclusive Biomek software. Features such as tip definitions, pipetting techniques and templates, and color-coded tip racks are built into Biomek software to make setup, method writing, and operation efficient and convenient. As a crucial component to the most advanced liquid handling systems, Biomek tips ensure high performance and quality control for the most exacting applications, including: genetic analysis, protein research, cellular analysis, and drug discovery. Biomek tips are manufactured in a controlled manufacturing environment to minimize facility bio-burden. Our pipette tips, racks, and rack covers are molded of premium-grade, virgin polypropylene to provide chemical resistance during sample preparation and to ensure only the highest quality product. Statistical process control (SPC) product sampling is conducted and samples are tested for biological parameters. The Most Critical Point of Contact BIOMEK PIPETTE TIPS
RNase- and DNase-free Ensures high quality nucleic acid purification and reliable nucleic acid testing results. DNA-free (human & mouse)/PCR inhibition Confirms the absence of both human and mouse DNA contaminants that result in erroneous results and interfere with the PCR. Endotoxin-free Addresses endotoxin-sensitive applications, such as cell transformations and preparative protocols that use extracted biological material for in vivo experimentation. Trace metal-free Testing of the base, polypropylene tip resin reduces interferences due to chelation and denaturation. Biomek pipette tips from Beckman Coulter are certified to be: 1 4 3 2
For the best in system performance, use only Beckman Coulter validated tips on Biomek workstations.
BIOLOGICAL TEST METHODOLOGIES RNase- and DNase-free certification
Tips are certified to be nuclease free. Tip extracts are incubated with the appropriate nucleic acid for 1 hour at 37°C, and then evaluated by agarose gel electrophoresis for integrity. The added RNA and DNA standards must be completely intact with no sign of degradation. This method tests to a sensitivity of 10 -9 Kunitz Units/μL for RNase and 10 -7 Kunitz Units/μL for DNase. DNA-free (human & mouse) certification/PCR inhibition A multiplex PCR reaction containing primers specific for both human and mouse genomic DNA is performed on the tip extracts. After amplification, no PCR product bands must be detectable, indicating no contaminating mouse or human DNA. Test sensitivity is 30 pg. To test for PCR inhibition, the tip extract is added to a reaction containing DNA template. After amplification, PCR products that represent a human DNA band and a mouse DNA band must be detectable; demonstrating that no inhibition effect is due to the tip extract. Endotoxin-free certification Products are extracted and tested for the presence of bacterial endotoxins using the Limulus amebocyte lysate (LAL) gel clot method. The test is sensitive to 0.06 EU/mL. Trace metal-free certification Manufacturing procedures require all materials used in tip production to be trace metals free. Polypropylene raw material is subject to lot certification and tested according to U.S. Pharmacopoeia guidelines.
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STORAGE CONDITIONS Biomek Tips are high-precision polypropylene components intended for use on Biomek Automated Workstations. The pipette tips are verified and validated at the systems level on multiple Biomek instruments to ensure compatibility of design and performance. Validation and performance of the systems are dependent on proper storage of these precision components.
STERILE (OR PRE-STERILE) PRODUCT
STERILE O
STERILE EO
STERILE R
STERILE R
Due to the precise geometries and extreme tolerances required of Biomek Tips in automated systems, Beckman Coulter offers sterilized product, which is controlled under validated ethylene oxide or irradiation processes, for those applications requiring sterile liquid handling. Products designated as “sterile or pre-sterile” are sterilized in accordance with ANSI/AAMI/ISO 11135 or 11137 guidelines, as appropriate. The sterilization processes certify a sterility assurance level (SAL) of 10 -6 . Autoclaving of Biomek Tips is not recommended, nor supported, due to the adverse affects it can have on the form and performance of the pipette tip. Environmental Awareness & Sustainability Biomek tips, racks, and rack covers are molded from polypropylene resin to provide chemical resistance during sample preparation. The Society of the Plastics Industry (SPI) identifies polypropylene with an internationally recognized resin identification code number of 5 for recycling purposes.
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STERILE EO STERILE EO STERILE EO It is recommended that Biomek Tips be stored in a clean and dry laboratory environment at room temperature. Exposure to high temperatures, radiation or vaporous chemicals/solvents may result in the deterioration of the design of the tip, and therefore its performance. STERILE R STERILE R STERILE R
Biomek tip packaging is comprised of 100% recyclable corrugated fiberboard.
© 2016 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com AAG-2181FLY12.16
BIOMEK TIPS AND LABWARE THE MOST CRITICAL POINT OF CONTACT.
TABLE OF CONTENTS
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Biomek Tips and Labware
Biomek 4000 and 3000 Pipette Tips
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14
Biomek i-Series Pipette Tips
Biomek 3000 and 2000 Pipette Tips
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15
Biomek i-Series Conductive Pipette Tips
Biomek Span-8 Fixed Probes
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8
Biomek AP384 Pipette Tips
Biomek Micro- and Deep-Well Plates
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17
Biomek AP96 Pipette Tips
Biomek Modular Reservoir System
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10
Biomek Span-8 Pipette Tips
Biomek Labware and Accessories
12
Biomek Span-8 Conductive Pipette Tips
Note: The use of the terms “pre-sterile” versus “sterile,” and “barrier” versus “filter,” respectively, are equivalent relative to product functionality and performance.
Accelerated workflows. Faster discovery.
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BIOMEK TIPS AND LABWARE Excellence for the critical point of contact.
From disposable pipette tips to microplates and everything in between, we provide a full suite of accurate, precise performance you can depend on. Biomek disposable tips from Beckman Coulter are the only tips designed, validated and approved for Biomek Liquid Handlers. Manufactured under stringent manufacturing processes, we guarantee proper fit and performance of Biomek tips on your Biomek liquid handler. Tip definitions, pipetting techniques, templates and color-coded tip racks are integrated into Biomek software to facilitate method development.
Tip definitions, pipetting techniques, templates, and color-coded tip racks are built into Biomek software to make setup, method writing and operation even more efficient. As a crucial component to the most advanced liquid handling systems, Biomek tips from Beckman Coulter ensure the highest performance and quality control for the most exacting applications such as genetic analysis, protein research, cellular analysis and drug discovery.
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To order, visit beckman.com or contact your local representative.
Biomek i-Series Pipette Tips Non-conductive tips for use with 60 µL Multichannel-384 Head.
30 µL PIPETTE TIPS Comparable to P30 Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case
B85739
Non-sterile
30 µL
B85745
Sterile
30 μL
B85749
Sterile filtered
25 μL
50 µL PIPETTE TIPS Comparable to P30XL Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case
B85753
Non-sterile
50 μL
B85756
Sterile
50 μL
B85760
Sterile filtered
40 μL
Accelerated workflows. Faster discovery.
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Biomek i-Series Pipette Tips Non-conductive tips for use with 300 & 1200 μL Multichannel-96 Heads & Span-8 Pod.
80 µL PIPETTE TIPS Comparable to P20 Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B85764
Non-sterile
80 μL
B85767
Sterile
80 μL
B85771
Sterile filtered
40 μL
90 µL PIPETTE TIPS Comparable to P50 Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B85881
Non-sterile
90 μL
B85884
Sterile
90 μL
B85888
Sterile filtered
50 μL
230 µL PIPETTE TIPS Comparable to P250 Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B85903
Non-sterile
230 μL
B85906
Sterile
230 μL
B85911
Sterile filtered
190 μL
230 µL WIDE BORE PIPETTE TIPS Comparable to P250 Wide Bore Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B85926
Wide bore, Non-sterile
230 μL
B85929
Wide bore, Sterile
230 μL
B85936
Wide bore, Sterile filtered
190 μL
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To order, visit beckman.com or contact your local representative.
Biomek i-Series Pipette Tips Non-conductive tips for use with 300 & 1200 μL Multichannel-96 Heads & Span-8 Pod.
1070µL PIPETTE TIPS Comparable to P1000 Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case
B85940
Non-sterile
1070 μL
B85945
Sterile
1070 μL
B85955
Sterile filtered
1025 μL
1070µL WIDE BORE PIPETTE TIPS Comparable to P1000 Wide Bore Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case
B85971
1070 μL
Wide bore, non-sterile
B85975
1070 μL
Wide bore, sterile
B85981
1025 μL
Wide bore, sterile filtered
Accelerated workflows. Faster discovery.
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Biomek i-Series Pipette Tips Conductive tips for use with Span-8 Pod to enable Liquid Level Sensing.
80µL CONDUCTIVE PIPETTE TIPS Comparable to P20 Conductive Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B85775
Conductive, Non-sterile
80 μL
B85872
Conductive, Sterile
80 μL
B85877
Conductive, Sterile filtered
40 μL
90µL CONDUCTIVE PIPETTE TIPS Comparable to P50 Conductive Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B85892
Conductive, Non-sterile
90 μL
B85894
Conductive, Sterile
90 μL
B85899
Conductive, Sterile filtered
50 μL
230µL CONDUCTIVE PIPETTE TIPS Comparable to P250 Conductive Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B85915
Conductive, Non-sterile
230 μL
B85917
Conductive, Sterile
230 μL
B85922
Conductive, Sterile filtered
190 μL
1070µL CONDUCTIVE PIPETTE TIPS Comparable to P1000 Conductive Tips for Biomek NX p /FX p instruments
Part No.
Description
Capacity
Packaging
96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case
B85959
Conductive, Non-sterile
1070 μL
B85961
Conductive, Sterile
1070 μL
B85965
Conductive, Sterile filtered
1025 μL
7
To order, visit beckman.com or contact your local representative.
Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek AP384 Multichannel Head.
P30 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case
719222
Non-sterile
30 μL
719223
Sterile
30 μL
394263
Sterile filtered
15 μL
P30XL PIPETTE TIPS
Part No.
Description
Capacity
Packaging
384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case
A22287
Non-sterile
50 μL
A22288
Sterile
50 μL
B98224
Sterile filtered
40 μL
Accelerated workflows. Faster discovery.
8
Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek AP96 Multichannel Heads.
P20 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
717254
Non-sterile
100 μL
717255
Sterile
100 μL
717256
Sterile filtered
20 μL
P50 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
A21578
Non-sterile
90 μL
A21582
Sterile
90 μL
A21586
Sterile filtered
50 μL
P250 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
717251
Non-sterile
220 μL
717252
Sterile
220 μL
717253
Sterile filtered
125 μL
P250 WIDE BORE PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B01101
Wide bore, Non-sterile
220 μL
B01102
Wide bore, Sterile
220 μL
B01103
Wide bore, Sterile filtered
170 μL
9
To order, visit beckman.com or contact your local representative.
Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek Span-8 Pod.
P20 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
379504
Non-sterile
100 μL
379505
Sterile
100 μL
379506
Sterile filtered
20 μL
P50 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
A21578
Non-sterile
90 μL
A21582
Sterile
90 μL
A21586
Sterile filtered
50 μL
P250 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
379501
Non-sterile
220 μL
379502
Sterile
220 μL
379503
Sterile filtered
125 μL
P250 WIDE BORE PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B01101
Wide bore, Non-sterile
220 μL
B01102
Wide bore, Sterile
220 μL
B01103
Wide bore, Sterile filtered
170 μL
Accelerated workflows. Faster discovery.
10
Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek Span-8 Pod.
P1000 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case
B01122
Non-sterile
1050 μL
B01123
Sterile
1050 μL
B01124
Sterile filtered
1025 μL
P1000 WIDE BORE PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case
B01112
Wide bore, Non-sterile
1050 μL
B01113
Wide bore, Sterile
1050 μL
B01114
Wide bore, Sterile filtered
1025 μL
11
To order, visit beckman.com or contact your local representative.
Biomek NX P /FX P Pipette Tips Conductive tips for use with Biomek Span-8 Pod to enable Liquid Level Sensing.
P20 CONDUCTIVE PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
719012
Non-sterile
100 μL
719013
Sterile
100 μL
394622
Sterile filtered
20 μL
P50 CONDUCTIVE PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
B01089
Non-sterile
90 μL
B01090
Sterile
90 μL
B01091
Sterile filtered
50 μL
P250 CONDUCTIVE PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case
719010
Non-sterile
220 μL
719011
Sterile
220 μL
394627
Sterile filtered
125 μL
P1000 CONDUCTIVE PIPETTE TIPS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case
987935
Non-sterile
1050 μL
987936
Sterile
1050 μL
987925
Sterile filtered
1025 μL
Accelerated workflows. Faster discovery.
12
Biomek 4000 and 3000 Pipette Tips Non-conductive tips for use with the Biomek 4000 and 3000 workstations.
FOR USE WITH P20 AND MP20 PIPETTE TOOLS
Part No.
Description
Capacity
Packaging
96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case Packaging
717254
P20 tips, Non-sterile
100 μL
717255
P20 tips, Sterile
100 μL
717256
P20 tips, Sterile filtered
20 μL
A21578
P50 tips, Non-sterile
90 μL
A21582
P50 tips, Sterile
90 μL
A21586
P50 tips, Sterile filtered
50 μL
FOR USE WITH P200L AND MP200 PIPETTE TOOLS
Part No.
Description
Capacity
A21578
P50 tips, Non-sterile
90 μL
A21582
P50 tips, Sterile
90 μL
A21586
P50 tips, Sterile filtered
50 μL
717251
P250 tips, Non-sterile
220 μL
717252
P250 tips, Sterile
220 μL
717253
P250 tips, Sterile filtered
125 μL
B01101
P250 wide bore tips, Non-sterile
220 μL
B01102
P250 wide bore tips, Sterile
220 μL
B01103
P250 wide bore tips, Sterile filtered
170 μL
FOR USE WITH P1000SL AND MP1000 PIPETTE TOOLS (BIOMEK 4000)
Part No.
Description
Capacity
Packaging
96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case
B01122
P1000 tips, Non-sterile
1050 μL
B01123
P1000 tips, Sterile
1050 μL
B01124
P1000 tips, Sterile filtered
1025 μL
B01112
P1000 wide bore tips, Non-sterile
1050 μL
B01113
P1000 wide bore tips, Sterile
1050 μL
B01114
P1000 wide bore tips, Sterile filtered
1025 μL
Filtered tips are not compatible with tip and liquid-level sensing on the Biomek 3000 and 4000 workstations.
13
To order, visit beckman.com or contact your local representative.
Biomek 3000 and 2000 Pipette Tips Non-conductive tips for use with the Biomek 3000 and 2000 workstations.
FOR USE WITH P1000L PIPETTE TOOL (BIOMEK 3000)
Part No.
Description
Capacity
Packaging
72 tips/tip box, 5 tip boxes/case
373688
P1000 tips, Non-sterile
1000 μL
609899
P1000 tips, Non-sterile, Bulk
1000 μL
1,000 tips/bag
72 tips/tip box, 5 tip boxes/case
373687
P1000 tips, Sterile
1000 μL
Biomek 2000 Pipette Tips Non-conductive tips for use with Biomek 2000 workstations.
P20 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
609044
Non-sterile
20 μL
96 tips/tip box, 10 tip boxes/case
609045
Non-sterile, Bulk
20 μL
1000 tips/bag
609042
Sterile
20 μL
96 tips/tip box, 10 tip boxes/case
609043
Sterile filtered
20 μL
96 tips/tip box, 10 tip boxes/case
P250 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
372654
Non-sterile
200 μL
96 tips/tip box, 10 tip boxes/case
373689
Non-sterile, Bulk
200 μL
1000 tips/bag
372655
Sterile
200 μL
96 tips/tip box, 10 tip boxes/case
140505
Sterile filtered
125 μL
96 tips/tip box, 10 tip boxes/case
P1000 PIPETTE TIPS
Part No.
Description
Capacity
Packaging
373688
Non-sterile
1000 μL
72 tips/tip box, 5 tip boxes/case
609899
Non-sterile, Bulk
1000 μL
1000 tips/bag
373687
Sterile
1000 μL
72 tips/tip box, 5 tip boxes/case
Barrer tips are not compatible with tip and liquid-level sensing on the Biomek 2000 workstations.
Accelerated workflows. Faster discovery.
14
Biomek Span-8 Fixed Probes For use with the Biomek Span-8 Pod.
FIXED PROBES
Part No.
Description
Probe Length
Packaging
719543
Stainless Steel Fixed Probe*
60 mm
1/case
719808
Stainless Steel Fixed Probe*
60 mm
8/case
719544
Stainless Steel Fixed Probe*
100 mm
1/case
719810
Stainless Steel Fixed Probe*
100 mm
8/case
A34967
Teflon-Coated Fixed Probe
60 mm
1/case
A39379
Teflon-Coated Fixed Probe
60 mm
8/case
A34966
Teflon-Coated Fixed Probe
100 mm
1/case
A39377
Teflon-Coated Fixed Probe
100 mm
8/case
717931
Teflon-Coated Fixed Probe*
60 mm
1/case
719807
Teflon-Coated Fixed Probe*
60 mm
8/case
717933
Teflon-Coated Fixed Probe*
100 mm
1/case
719809
Teflon-Coated Fixed Probe*
100 mm
8/case
987873
Stainless Steel Piercing Probe*
120 mm
1/pack
987872
Stainless Steel Piercing Probe*
120 mm
4/pack
987870
Stainless Steel Piercing Probe*
120 mm
8/pack
A49675
Large Volume Tubing Kit†
—
—
* Requires Biomek Large Volume Tubing Kit (Part No. A49675). † The Biomek Large Volume Tubing Kit includes tubing and other parts necessary to convert a system with low-volume tubing to large-volume tubing for use with large-volume syringes or stainless steel piercing probes. 100 mm and 120 mm probes are compatible with Biomek NX P /FX P and i-Series Span-8 Pods. Teflon is a registered trademark of E.I. du Pont de Nemours and Company.
15
To order, visit beckman.com or contact your local representative.
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