Biomek iSeries

TABLE OF CONTENTS

TABLE OF CONTENTS

March 2018

Brochures & Flyers • i-Series Automated Workstations Brochure........................................................................................................................... 1 • Biomek Wide Bore Tips: Ensure Biological Integrity of Samples.............................................................................. 13 • Biomek Pipette Tips: The Most Critical Point of Contact.............................................................................................. 15 • Biomek Tips and Labware Brochure....................................................................................................................................... 17 • Biomek i-Series Pipetting Performance Capabilities..................................................................................................... 37 • Biomek-Automated Genomic Solutions: Take your next step with confidence................................................ 39 • Biomek-Automated NGS Library Construction Methods............................................................................................ 47 • i-Series Drug Discovery and Basic research Brochure ................................................................................................. 49 • Integrated Solutions Device List.............................................................................................................................................. 57 • Genomics Products Portfolio Overview................................................................................................................................ 62 Application & Technical Notes • Simple, yet effective – automating Bradford assays ..................................................................................................... 65 • Automated Cell Plating and Growth Assays...................................................................................................................... 67 • Flexible ELISA automation with the Biomek i5 Workstation..................................................................................... 69 • Efficient Factorial Optimization of Transfection Conditions....................................................................................... 71 • Fully-Automated Cellular Analysis by Flow Cytometry................................................................................................ 74 • Biomek Automated Genomic Sample Prep Accelerates Research......................................................................... 76 • Biomek i-Series Automated Beckman Coulter AMPure XP PCR Purification System.................................... 81 • Biomek i-Series Automated FormaPure DNA Extraction kit for FFPE Samples.............................................. 84 • Biomek i-Series Automated Illumina TruSeq DNA PCR-Free.................................................................................... 87 • Biomek i-Series Automated Illumina TruSeq Exome Library Prep Kit................................................................... 90 • Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips.................................................................................................................... 94 • Consistent Cell Maintenance and Plating through Automation .............................................................................. 98 • Biomek Automated TruSight Tumor 170 Assay - Illumina Qualified........................................................................101 WorkFlow Overview • Continuous Cell Culture: Automating Cell-Based Processes................................................................................... 105 • High Throughput Screening: Make an impact by removing the barriers........................................................... 107 • Transfection: Efficient, high-quality results with automated transfection methods..................................... 109 • Cell Culture: Automating Cell Maintenance......................................................................................................................... 111 • Cell-Based Assays: One platform to meet your evolving assay needs................................................................. 113 • Cell Line Development: Boost Your Labs’ Efficiency..................................................................................................... 115

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

For Beckman Coulter’s worldwide office locations and phone numbers, please visit beckmancoulter.com/contact

© 2018 Beckman Coulter, Inc.

DESIGNED FOR YOUR EVOLVING WORKFLOW PRIORITIES Biomek i-Series Automated Workstations

ACCELERATED WORKFLOWS . Faster Discovery.

YOU TALKED. WE LISTENED . Your priorities continue to evolve; ours never change. Because our priority is you. Your input and our experience led to the engineering of a liquid handling platform that enables you to get the trusted, reliable results you need from your application workflows. From your input, some recurring themes appeared. And from those themes emerged a common foundation—for hardware, software, accessories, consumables and support—that’s built-on: • Simplification, so you can focus more on science and less on managing your tools • Efficiency, to help you optimize productivity, increase walk-away time and learn more—faster • Adaptability, so the technology you invest in can grow with you, not grow obsolete • Reliability and support, to help maximize instrument uptime so your research is always moving forward On that customer-driven foundation, we’re building the future of laboratory automation.

The benefits of an automated workstation from Beckman Coulter Life Sciences come from more than just an instrument. They’re realized thanks to synergy between:

• Hardware • Software • Accessories • Consumables • Service and support

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ACCELERATED WORKFLOWS . Faster Discovery.

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ADVANCING SCIENCE BY ACCELERATING A WIDE RANGE OF APPLICATIONS Automation drives acceleration. This is true for pharma, biotech, academia and government-sponsored research—virtually any lab focused on genomics, cell biology, proteomics, drug discovery, forensics and related research areas. A sampling of drug discovery and basic research application areas that can benefit from Biomek automation:

• Cell-based assays • High-throughput screening

• Continuous cell culture • Biologic bioanalysis • Proteomics, lipidomics, metabolomics • Synthetic biology • 3D cell models • Compound handling • Nucleic acid sample prep °° DNA/RNA isolation °° DNA/RNA clean-up °° DNA size selection °° Plasmid prep • Sanger sequencing °° Big dye clean-up • qPCR/PCR setup

• High-content screening • Cell line development

• Cell culture • Transfection

A sampling of genomics application areas that can benefit from Biomek automation:

• Next-generation sequencing sample prep °° Whole genome sequencing °° Transcriptome sequencing °° Target/exome capture °° Amplicon sequencing °° Cancer panels °° HLA typing • Microarray sample prep °° Genotyping °° Gene expression

Many fields of research continue to accelerate at a rapid pace. Our commitment is to provide the innovative technology you need to maintain that pace—by simplifying your workflows, improving efficiency, increasing walk-away time and decreasing downtime.

The most recent example of this commitment: Biomek i-Series Automated Workstations1.

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EXPLORE THE BIOMEK i-SERIES AUTOMATED Representing the best of what has made Biomek workstations an industry leader—combined with enhancements suggested by customers around the globe—Biomek i-Series Automated Workstations have been designed to optimize dependability and walk-away time in mid- to high-throughput labs.

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Simplicity so you can focus more on science Bright, multiple color- and pattern-coded status light bar alerts you to the instrument’s current mode, even from across the room. Light Curtain provides key safety feature during operation and method development. Internal LED light illuminates the instrument deck for easy access and monitoring of your workspace status. 1 2 3

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Efficiency to help deliver higher productivity

Rotating gripper with unique offset finger design optimizes access to high-density decks, enabling more efficient workflows. Linear motion control increases positional accuracy for pipetting access to high-density labware. Large-volume, 1 mL multichannel pipetting head expedites sample transfers and enables more efficient mixing steps.

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Biomek i7 Automated Workstation

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ACCELERATED WORKFLOWS . Faster Discovery.

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WORKSTATIONS

BIOMEK i-SERIES WORKSTATIONS ENABLE “4D INTEGRATION” With access from 4 directions—

right, left, back and below—i- Series workstations make it easy to integrate a growing menu of devices, components and accessories. Your Beckman Coulter team can help you with standard integrations.

Adaptability to extend scale and reach Grid-based deck with simple accessory installation enables quick workflow changes. Spacious, open-platform design enables access from all sides to enable integration of adjacent-to-deck and off-deck processing elements (e.g., analytical devices, external storage/incubation units, and labware feeders). 7 8 Trusted reliability and support to reduce downtime Safeguard sample and reagent integrity from air particulates with enclosed versions of the Biomek i-Series Automated Workstations. Vertical sliding door on enclosure provides front access without aisle obstruction. Onboard cameras enable live broadcast and on-error video capture to expedite response time and system diagnosis. Span-8 pipetting with independent probe calibration for volume transfers from 0.5 to 5,000 μL. 9 10 11 12

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YOUR APPLICATIONS

ACCESSORIES TO ENABLE

Build a system to suit your needs. Choose the accessories you need to maximize the efficiency and reliability delivered by a Biomek i-Series Automated Workstation.

Multichannel heads In addition to Span-8 pipetting, three multichannel head options are available:

RANGE

MC-384, 60 μL

0.5 μL - 60 μL

MC-96, 300 μL

1 μL - 300 μL

MC-96, 1,200 μL

5 μL - 1,200 μL

For more information on compatible tips, request a copy of Beckman Coulter’s Biomek Tips and Labware catalog.

Automated Labware Positioners (ALPs)

Designed to fit seamlessly onto the deck of any Biomek workstation, ALPs from Beckman Coulter Life Sciences perform application-specific tasks, such as: • Tip washing • Shaking • Heating/cooling • Reagent reservoirs • And a host of other options depending on your application

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ACCELERATED WORKFLOWS . Faster Discovery.

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Device integrations and customization services To further enhance the capabilities of your Biomek i-Series Automated Workstation, you can choose from a list of device integrations or ask for our customization services. Your support team will include experts who’ve integrated hundreds of third-party devices to meet a large variety of workflow needs.

Additional components • Fly-by barcode reader for positive sample ID, process and tracking of microplates • Labware feeders • Instrument carts (pictured) and tables • And a host of others depending on your specific needs

Biomek i5 Automated Workstation

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INDUSTRY-LEADING BIOMEK SOFTWARE FOR i-SERIES THAT DELIVERS WORKFLOW INTELLIGENCE The latest version of customer-acclaimed Biomek software, Biomek i-Series software maximizes ROI by giving you unprecedented control over your liquid handler.

Method authoring • Sets the standard for method authoring with a point-and-click interface; no advanced software expertise required. • Saves time and consumables with Biomek’s visual editor, which validates your method in real time as you create it, and Biomek’s 3D simulator, which demonstrates exactly how your method will execute. • Provides full control over movement of the tip in the well to match even the most complex manual pipettingmotions. Ease of operation • Improves accuracy and reduces errors by giving operators step-by-step guidance for placing labware on the deck. • Makes it easy for lab technicians to launch/monitor methods by providing a simple point-and-click user interface. • Lets you “lock down” the instrument and protect validated methods from being altered inadvertently by operators. • Supports regulated laboratories and multi-user environments by controlling access using electronic signatures. • Enables remote instrument monitoring using any device with a Google Chrome browser. Data management • Captures the data you need to validate your processes and help ensure reproducible results. • Integrates with LIMS systems to import work orders and export data. • Seamlessly transfers data between methods so you can easily generate run, labware and sample reports at any time. • Data-driven methods select appropriate actions during execution based on sample data generated in real time.

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ACCELERATED WORKFLOWS . Faster Discovery.

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Additional software solutions include:

Simplify data management with DART Automation systems generate large quantities of data to manage. DART simplifies workflow data by transforming it into powerful information to inform your research decisions.

Streamline workflows with SAMI EX Automate full workflows with a process control supporting a wide variety of applications. Use the power of a planning scheduler and sample data drill-down to manage your assay.

Easily automate your most complex workflows with SAMI Process Management

This calendar-format tool enables the addition, monitoring and planning of SAMI EX methods and other events as part of user-defined processes.

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BIOMEK PIPETTE TIPS: THE MOST CRITICAL POINT OF CONTACT

All Biomek workstations are optimized to perform as complete systems. That’s why only Biomek pipette tips from Beckman Coulter Life Sciences are validated and approved for use with our workstations. Biomek pipette tips, racks and rack covers are molded from premium-grade, virgin polypropylene to provide chemical resistance during sample preparation, and to ensure the highest performance and quality control for your most exacting applications. To make setup, method writing, and operation easy and efficient, Biomek software already includes point-and-click options for Biomek tip definitions, pipetting techniques and templates, and color-coded Biomek tip racks.

Only Biomek pipette tips are validated and approved for use with Biomek workstations.

Biomek pipette tips from Beckman Coulter are certified to be: • RNase- and DNase-free Ensures high-quality nucleic acid purification and reliable nucleic acid testing results. • DNA-free (human & mouse)/PCR inhibition Confirms the absence of both human and mouse DNA contaminants that result in erroneous results and interfere with the PCR. • Endotoxin-free Addresses endotoxin-sensitive applications, such as cell transformations and preparative protocols that use extracted biological material for in vivo experimentation. • Trace metal-free

Testing of the base, polypropylene tip resin reduces interferences due to chelation and denaturation.

For more information on Biomek tips and accessories, request a copy of Beckman Coulter’s Biomek Tips and Labware catalog or visit beckman.com .

i - Series 10 | ACCELERATED WORKFLOWS . Faster Discovery.

WORLD-CLASS TRAINING, SERVICE AND SUPPORT ARE INSTRUMENTAL TO SUCCESS

From online classes or instructor-led training, to on-site application support – we’re committed to doing everything possible to ensure that you can operate your Biomek workstation with confidence. In addition, when you choose a Biomek i-Series Automated Workstation from Beckman Coulter Life Sciences, you can expect support and maintenance from an organization that’s rated higher for technical support than any major competitor. Further building on our commitment to world-class service, the PROService Remote Monitoring System is now available to help maximize system uptime by shortening service calls and expediting repair times.

Instructor-led training or on-line classes help ensure that you can operate your i-Series workstation with confidence.

Our customer support center directs a team of engineers with extensive product knowledge who can trouble- shoot issues quickly and efficiently.

To help maximize instrument uptime, the PROService Remote Monitoring System can use built-in cameras to show our support team a secure view of your i-Series workstation.

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SYSTEM SPECIFICATIONS FOR BIOMEK i-SERIES AUTOMATED WORKSTATIONS

ITEM

WITHOUT ENCLOSURE

WITH ENCLOSURE

Width: 112 cm (44”) Depth: 81 cm (32”) Height: 104 cm (41”) Width: 170 cm (67”) Depth: 81 cm (32”) Height: 104 cm (41”)

Width: 112 cm (44”) Depth: 81 cm (32”) Height: 112 cm (44”) Width: 170 cm (67”) Depth: 81 cm (32”) Height: 112 cm (44”)

Dimensions for Biomek i5 Base Unit

Dimensions for Biomek i7 Base Unit

Maximum Height w/Door Open

N/A

147 cm (58”)

Weight: Biomek i5 Base Unit

Multichannel

155 kg (341 lbs)

181 kg (399 lbs)

Span-8

146 kg (322 lbs)

172 kg (379 lbs)

Weight: Biomek i7 Base Unit

Multichannel

199 kg (439 lbs)

234 kg (516 lbs)

Dual Multichannel

234 kg (516 lbs)

269 kg (593 lbs)

Span-8

190 kg (419 lbs)

225 kg (496 lbs)

Hybrid (Multichannel + Span-8)

225 kg (496 lbs)

260 kg (573 lbs)

Introducing the i-Series to our family of Biomek Automated Workstations is just the beginning of our journey together. Biomek hardware, software and our growing applications portfolio will continue to evolve with your priorities in mind. Visit biomek.beckman.com or speak with your Beckman Coulter representative to learn more.

1 Currently in development © 2017 Beckman Coulter, Inc. All rights reserved.

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com AAG-2215SB01.17

Biomek Wide Bore Pipette Tips minimize the inadvertent harm, which can be applied to native biological sample matrices during sample preparation. Loss of biological sample integrity can occur when pipetting fragile cell suspensions, hybridomas, embryoid bodies, algal coagulates, protein aggregates, and genomic materials due to the pipette tips’ small internal orifice diameter and the speed of aspiration and dispense during pipetting. Biomek Wide Bore Tips have been designed to address such sample preparation challenges with a pipetting orifice twice the size of standard pipette tips.¹ Ensure Biological Integrity of Samples BIOMEK WIDE BORE PIPETTE TIPS

Ensure biological integrity of samples Reduce flow-induced shear strain on native biological sample matrices with wide bore tips. Minimize DNA shearing Avoid the risk of inadvertent DNA shearing, while preparing samples during nucleic acid extraction and purification protocols. Improve sample throughput Increase throughput when pipetting viscous reagent solutions, such as glycerol stocks, or bead mixtures. Minimize cell lysis Prevent inadvertent cell lysis when pipetting fragile cell suspensions, hybridomas, or embryoid bodies. Reduce aggregate disruptions Maintain the natural state of protein aggregates in therapeutic formulation studies and algal coagulated in biomass studies. Prevent tip clogging Avoid obstructions due to heavy particulate or fibrous sample matrices as encountered in biomass studies and agricultural research.

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Comparison of Standard vs. Wide Bore Orifice Pipette Tip Diameters

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For the best in system performance, use only Beckman Coulter validated tips on Biomek workstations.

1.4 mm * (Wide Bore)

0.6 mm * (Standard)

* Enlarged for illustrative purposes only.

Improved Pipetting Efficiency The capability to transfer a full 1 mL of samples or reagents in a single pipetting step provides greater efficiency and timesavings during sample preparation. The 1 mL tips are commonly used for large volume transfers applied to nucleic acid extraction and purification protocols, “seed and feed” cellular applications, and general reagent pipetting. The overall length of the tips allows access to the full contents of long, narrow tubes, such as 12 x 75 mm and 16 x 100 mm test tubes, 15 and 50 mL centrifugation tubes, and 1 and 2 mL deep-well plates. For added confidence in Biomek pipetting performance during method development, the non-conductive, Wide

Bore Tips offer the advantage of being clear (versus black, conductive) to allow for visual inspection of aspirated volumes. With the clear tips, researchers are able to see how much volume they have aspirated into the tips, and the uniformity of aspirated volume across tips. In addition, Biomek Wide Bore Tips reduce sample preparation times by allowing faster pipetting speeds of viscous reagent solutions, such as glycerol stocks, or bead mixtures. The larger diameter bore also minimizes tip clogging when pipetting particulate- laden or fibrous sample matrices as those found in biomass studies and agricultural research.

Biomek Wide Bore Pipette Tips Ordering Information

STERILE (PRE-STERILE)

STERILEWITH FILTER (BARRIER)

NON-STERILE

PACKAGING

For use with Biomek 4000, and Biomek NX p and FX p Span-8 Automated Workstations.*

96 tips/tip box, 10 tip boxes/case

P250

B01101

B01102

B01103

96 tips/tip box, 5 tip boxes/case

P1000

B01112

B01113

B01114

For use with Biomek i-Series Automated Workstations.

96 tips/tip box, 10 tip boxes/case

230/190 μL

B85926

B85929

B85936

96 tips/tip box, 5 tip boxes/case

1070/1025 μL

B85971

B85975

B85981

Environmental Awareness & Sustainability Biomek tips, racks, and rack covers are molded from polypropylene resin to provide chemical resistance during sample preparation. The Society of the Plastics Industry (SPI) identifies polypropylene with an internationally recognized resin identification code number of 5 for recycling purposes.

Bio-certifications Biomek tips are certified to be free of RNase, DNase, DNA (human and mouse) / PCR inhibition, endotoxins, and trace metals.

For additional information, request: Technical Information Bulletin, IB-17295A, Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate; Biomek Wide Bore vs. Standard Pipette Tips , Robert D. Lund, Beckman Coulter Life Sciences, Indianapolis, IN. ¹

Biomek tip packaging is comprised of 100% recyclable corrugated fiberboard.

*i-Series in development. © 2016 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com AAG-2182FLY12.16

Biomek pipette tips from Beckman Coulter are the only pipette tips validated and approved for use with Biomek workstations. Our tips work seamlessly with Biomek hardware and are fully integrated into our exclusive Biomek software. Features such as tip definitions, pipetting techniques and templates, and color-coded tip racks are built into Biomek software to make setup, method writing, and operation efficient and convenient. As a crucial component to the most advanced liquid handling systems, Biomek tips ensure high performance and quality control for the most exacting applications, including: genetic analysis, protein research, cellular analysis, and drug discovery. Biomek tips are manufactured in a controlled manufacturing environment to minimize facility bio-burden. Our pipette tips, racks, and rack covers are molded of premium-grade, virgin polypropylene to provide chemical resistance during sample preparation and to ensure only the highest quality product. Statistical process control (SPC) product sampling is conducted and samples are tested for biological parameters. The Most Critical Point of Contact BIOMEK PIPETTE TIPS

RNase- and DNase-free Ensures high quality nucleic acid purification and reliable nucleic acid testing results. DNA-free (human & mouse)/PCR inhibition Confirms the absence of both human and mouse DNA contaminants that result in erroneous results and interfere with the PCR. Endotoxin-free Addresses endotoxin-sensitive applications, such as cell transformations and preparative protocols that use extracted biological material for in vivo experimentation. Trace metal-free Testing of the base, polypropylene tip resin reduces interferences due to chelation and denaturation. Biomek pipette tips from Beckman Coulter are certified to be: 1 4 3 2

For the best in system performance, use only Beckman Coulter validated tips on Biomek workstations.

BIOLOGICAL TEST METHODOLOGIES RNase- and DNase-free certification

Tips are certified to be nuclease free. Tip extracts are incubated with the appropriate nucleic acid for 1 hour at 37°C, and then evaluated by agarose gel electrophoresis for integrity. The added RNA and DNA standards must be completely intact with no sign of degradation. This method tests to a sensitivity of 10 -9 Kunitz Units/μL for RNase and 10 -7 Kunitz Units/μL for DNase. DNA-free (human & mouse) certification/PCR inhibition A multiplex PCR reaction containing primers specific for both human and mouse genomic DNA is performed on the tip extracts. After amplification, no PCR product bands must be detectable, indicating no contaminating mouse or human DNA. Test sensitivity is 30 pg. To test for PCR inhibition, the tip extract is added to a reaction containing DNA template. After amplification, PCR products that represent a human DNA band and a mouse DNA band must be detectable; demonstrating that no inhibition effect is due to the tip extract. Endotoxin-free certification Products are extracted and tested for the presence of bacterial endotoxins using the Limulus amebocyte lysate (LAL) gel clot method. The test is sensitive to 0.06 EU/mL. Trace metal-free certification Manufacturing procedures require all materials used in tip production to be trace metals free. Polypropylene raw material is subject to lot certification and tested according to U.S. Pharmacopoeia guidelines.

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STORAGE CONDITIONS Biomek Tips are high-precision polypropylene components intended for use on Biomek Automated Workstations. The pipette tips are verified and validated at the systems level on multiple Biomek instruments to ensure compatibility of design and performance. Validation and performance of the systems are dependent on proper storage of these precision components.

STERILE (OR PRE-STERILE) PRODUCT

STERILE O

STERILE EO

STERILE R

STERILE R

Due to the precise geometries and extreme tolerances required of Biomek Tips in automated systems, Beckman Coulter offers sterilized product, which is controlled under validated ethylene oxide or irradiation processes, for those applications requiring sterile liquid handling. Products designated as “sterile or pre-sterile” are sterilized in accordance with ANSI/AAMI/ISO 11135 or 11137 guidelines, as appropriate. The sterilization processes certify a sterility assurance level (SAL) of 10 -6 . Autoclaving of Biomek Tips is not recommended, nor supported, due to the adverse affects it can have on the form and performance of the pipette tip. Environmental Awareness & Sustainability Biomek tips, racks, and rack covers are molded from polypropylene resin to provide chemical resistance during sample preparation. The Society of the Plastics Industry (SPI) identifies polypropylene with an internationally recognized resin identification code number of 5 for recycling purposes.

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STERILE EO STERILE EO STERILE EO It is recommended that Biomek Tips be stored in a clean and dry laboratory environment at room temperature. Exposure to high temperatures, radiation or vaporous chemicals/solvents may result in the deterioration of the design of the tip, and therefore its performance. STERILE R STERILE R STERILE R

Biomek tip packaging is comprised of 100% recyclable corrugated fiberboard.

© 2016 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com AAG-2181FLY12.16

BIOMEK TIPS AND LABWARE THE MOST CRITICAL POINT OF CONTACT.

TABLE OF CONTENTS

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Biomek Tips and Labware

Biomek 4000 and 3000 Pipette Tips

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Biomek i-Series Pipette Tips

Biomek 3000 and 2000 Pipette Tips

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15

Biomek i-Series Conductive Pipette Tips

Biomek Span-8 Fixed Probes

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8

Biomek AP384 Pipette Tips

Biomek Micro- and Deep-Well Plates

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17

Biomek AP96 Pipette Tips

Biomek Modular Reservoir System

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Biomek Span-8 Pipette Tips

Biomek Labware and Accessories

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Biomek Span-8 Conductive Pipette Tips

Note: The use of the terms “pre-sterile” versus “sterile,” and “barrier” versus “filter,” respectively, are equivalent relative to product functionality and performance.

Accelerated workflows. Faster discovery.

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BIOMEK TIPS AND LABWARE Excellence for the critical point of contact.

From disposable pipette tips to microplates and everything in between, we provide a full suite of accurate, precise performance you can depend on. Biomek disposable tips from Beckman Coulter are the only tips designed, validated and approved for Biomek Liquid Handlers. Manufactured under stringent manufacturing processes, we guarantee proper fit and performance of Biomek tips on your Biomek liquid handler. Tip definitions, pipetting techniques, templates and color-coded tip racks are integrated into Biomek software to facilitate method development.

Tip definitions, pipetting techniques, templates, and color-coded tip racks are built into Biomek software to make setup, method writing and operation even more efficient. As a crucial component to the most advanced liquid handling systems, Biomek tips from Beckman Coulter ensure the highest performance and quality control for the most exacting applications such as genetic analysis, protein research, cellular analysis and drug discovery.

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To order, visit beckman.com or contact your local representative.

Biomek i-Series Pipette Tips Non-conductive tips for use with 60 µL Multichannel-384 Head.

30 µL PIPETTE TIPS Comparable to P30 Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case

B85739

Non-sterile

30 µL

B85745

Sterile

30 μL

B85749

Sterile filtered

25 μL

50 µL PIPETTE TIPS Comparable to P30XL Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case

B85753

Non-sterile

50 μL

B85756

Sterile

50 μL

B85760

Sterile filtered

40 μL

Accelerated workflows. Faster discovery.

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Biomek i-Series Pipette Tips Non-conductive tips for use with 300 & 1200 μL Multichannel-96 Heads & Span-8 Pod.

80 µL PIPETTE TIPS Comparable to P20 Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B85764

Non-sterile

80 μL

B85767

Sterile

80 μL

B85771

Sterile filtered

40 μL

90 µL PIPETTE TIPS Comparable to P50 Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B85881

Non-sterile

90 μL

B85884

Sterile

90 μL

B85888

Sterile filtered

50 μL

230 µL PIPETTE TIPS Comparable to P250 Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B85903

Non-sterile

230 μL

B85906

Sterile

230 μL

B85911

Sterile filtered

190 μL

230 µL WIDE BORE PIPETTE TIPS Comparable to P250 Wide Bore Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B85926

Wide bore, Non-sterile

230 μL

B85929

Wide bore, Sterile

230 μL

B85936

Wide bore, Sterile filtered

190 μL

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To order, visit beckman.com or contact your local representative.

Biomek i-Series Pipette Tips Non-conductive tips for use with 300 & 1200 μL Multichannel-96 Heads & Span-8 Pod.

1070µL PIPETTE TIPS Comparable to P1000 Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case

B85940

Non-sterile

1070 μL

B85945

Sterile

1070 μL

B85955

Sterile filtered

1025 μL

1070µL WIDE BORE PIPETTE TIPS Comparable to P1000 Wide Bore Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case

B85971

1070 μL

Wide bore, non-sterile

B85975

1070 μL

Wide bore, sterile

B85981

1025 μL

Wide bore, sterile filtered

Accelerated workflows. Faster discovery.

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Biomek i-Series Pipette Tips Conductive tips for use with Span-8 Pod to enable Liquid Level Sensing.

80µL CONDUCTIVE PIPETTE TIPS Comparable to P20 Conductive Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B85775

Conductive, Non-sterile

80 μL

B85872

Conductive, Sterile

80 μL

B85877

Conductive, Sterile filtered

40 μL

90µL CONDUCTIVE PIPETTE TIPS Comparable to P50 Conductive Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B85892

Conductive, Non-sterile

90 μL

B85894

Conductive, Sterile

90 μL

B85899

Conductive, Sterile filtered

50 μL

230µL CONDUCTIVE PIPETTE TIPS Comparable to P250 Conductive Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B85915

Conductive, Non-sterile

230 μL

B85917

Conductive, Sterile

230 μL

B85922

Conductive, Sterile filtered

190 μL

1070µL CONDUCTIVE PIPETTE TIPS Comparable to P1000 Conductive Tips for Biomek NX p /FX p instruments

Part No.

Description

Capacity

Packaging

96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case

B85959

Conductive, Non-sterile

1070 μL

B85961

Conductive, Sterile

1070 μL

B85965

Conductive, Sterile filtered

1025 μL

7

To order, visit beckman.com or contact your local representative.

Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek AP384 Multichannel Head.

P30 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case

719222

Non-sterile

30 μL

719223

Sterile

30 μL

394263

Sterile filtered

15 μL

P30XL PIPETTE TIPS

Part No.

Description

Capacity

Packaging

384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case 384 tips/tip box, 10 tip boxes/case

A22287

Non-sterile

50 μL

A22288

Sterile

50 μL

B98224

Sterile filtered

40 μL

Accelerated workflows. Faster discovery.

8

Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek AP96 Multichannel Heads.

P20 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

717254

Non-sterile

100 μL

717255

Sterile

100 μL

717256

Sterile filtered

20 μL

P50 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

A21578

Non-sterile

90 μL

A21582

Sterile

90 μL

A21586

Sterile filtered

50 μL

P250 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

717251

Non-sterile

220 μL

717252

Sterile

220 μL

717253

Sterile filtered

125 μL

P250 WIDE BORE PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B01101

Wide bore, Non-sterile

220 μL

B01102

Wide bore, Sterile

220 μL

B01103

Wide bore, Sterile filtered

170 μL

9

To order, visit beckman.com or contact your local representative.

Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek Span-8 Pod.

P20 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

379504

Non-sterile

100 μL

379505

Sterile

100 μL

379506

Sterile filtered

20 μL

P50 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

A21578

Non-sterile

90 μL

A21582

Sterile

90 μL

A21586

Sterile filtered

50 μL

P250 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

379501

Non-sterile

220 μL

379502

Sterile

220 μL

379503

Sterile filtered

125 μL

P250 WIDE BORE PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B01101

Wide bore, Non-sterile

220 μL

B01102

Wide bore, Sterile

220 μL

B01103

Wide bore, Sterile filtered

170 μL

Accelerated workflows. Faster discovery.

10

Biomek NX P /FX P Pipette Tips Non-conductive tips for use with Biomek Span-8 Pod.

P1000 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case

B01122

Non-sterile

1050 μL

B01123

Sterile

1050 μL

B01124

Sterile filtered

1025 μL

P1000 WIDE BORE PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case

B01112

Wide bore, Non-sterile

1050 μL

B01113

Wide bore, Sterile

1050 μL

B01114

Wide bore, Sterile filtered

1025 μL

11

To order, visit beckman.com or contact your local representative.

Biomek NX P /FX P Pipette Tips Conductive tips for use with Biomek Span-8 Pod to enable Liquid Level Sensing.

P20 CONDUCTIVE PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

719012

Non-sterile

100 μL

719013

Sterile

100 μL

394622

Sterile filtered

20 μL

P50 CONDUCTIVE PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

B01089

Non-sterile

90 μL

B01090

Sterile

90 μL

B01091

Sterile filtered

50 μL

P250 CONDUCTIVE PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case

719010

Non-sterile

220 μL

719011

Sterile

220 μL

394627

Sterile filtered

125 μL

P1000 CONDUCTIVE PIPETTE TIPS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case

987935

Non-sterile

1050 μL

987936

Sterile

1050 μL

987925

Sterile filtered

1025 μL

Accelerated workflows. Faster discovery.

12

Biomek 4000 and 3000 Pipette Tips Non-conductive tips for use with the Biomek 4000 and 3000 workstations.

FOR USE WITH P20 AND MP20 PIPETTE TOOLS

Part No.

Description

Capacity

Packaging

96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case 96 tips/tip box, 10 tip boxes/case Packaging

717254

P20 tips, Non-sterile

100 μL

717255

P20 tips, Sterile

100 μL

717256

P20 tips, Sterile filtered

20 μL

A21578

P50 tips, Non-sterile

90 μL

A21582

P50 tips, Sterile

90 μL

A21586

P50 tips, Sterile filtered

50 μL

FOR USE WITH P200L AND MP200 PIPETTE TOOLS

Part No.

Description

Capacity

A21578

P50 tips, Non-sterile

90 μL

A21582

P50 tips, Sterile

90 μL

A21586

P50 tips, Sterile filtered

50 μL

717251

P250 tips, Non-sterile

220 μL

717252

P250 tips, Sterile

220 μL

717253

P250 tips, Sterile filtered

125 μL

B01101

P250 wide bore tips, Non-sterile

220 μL

B01102

P250 wide bore tips, Sterile

220 μL

B01103

P250 wide bore tips, Sterile filtered

170 μL

FOR USE WITH P1000SL AND MP1000 PIPETTE TOOLS (BIOMEK 4000)

Part No.

Description

Capacity

Packaging

96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case 96 tips/tip box, 5 tip boxes/case

B01122

P1000 tips, Non-sterile

1050 μL

B01123

P1000 tips, Sterile

1050 μL

B01124

P1000 tips, Sterile filtered

1025 μL

B01112

P1000 wide bore tips, Non-sterile

1050 μL

B01113

P1000 wide bore tips, Sterile

1050 μL

B01114

P1000 wide bore tips, Sterile filtered

1025 μL

Filtered tips are not compatible with tip and liquid-level sensing on the Biomek 3000 and 4000 workstations.

13

To order, visit beckman.com or contact your local representative.

Biomek 3000 and 2000 Pipette Tips Non-conductive tips for use with the Biomek 3000 and 2000 workstations.

FOR USE WITH P1000L PIPETTE TOOL (BIOMEK 3000)

Part No.

Description

Capacity

Packaging

72 tips/tip box, 5 tip boxes/case

373688

P1000 tips, Non-sterile

1000 μL

609899

P1000 tips, Non-sterile, Bulk

1000 μL

1,000 tips/bag

72 tips/tip box, 5 tip boxes/case

373687

P1000 tips, Sterile

1000 μL

Biomek 2000 Pipette Tips Non-conductive tips for use with Biomek 2000 workstations.

P20 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

609044

Non-sterile

20 μL

96 tips/tip box, 10 tip boxes/case

609045

Non-sterile, Bulk

20 μL

1000 tips/bag

609042

Sterile

20 μL

96 tips/tip box, 10 tip boxes/case

609043

Sterile filtered

20 μL

96 tips/tip box, 10 tip boxes/case

P250 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

372654

Non-sterile

200 μL

96 tips/tip box, 10 tip boxes/case

373689

Non-sterile, Bulk

200 μL

1000 tips/bag

372655

Sterile

200 μL

96 tips/tip box, 10 tip boxes/case

140505

Sterile filtered

125 μL

96 tips/tip box, 10 tip boxes/case

P1000 PIPETTE TIPS

Part No.

Description

Capacity

Packaging

373688

Non-sterile

1000 μL

72 tips/tip box, 5 tip boxes/case

609899

Non-sterile, Bulk

1000 μL

1000 tips/bag

373687

Sterile

1000 μL

72 tips/tip box, 5 tip boxes/case

Barrer tips are not compatible with tip and liquid-level sensing on the Biomek 2000 workstations.

Accelerated workflows. Faster discovery.

14

Biomek Span-8 Fixed Probes For use with the Biomek Span-8 Pod.

FIXED PROBES

Part No.

Description

Probe Length

Packaging

719543

Stainless Steel Fixed Probe*

60 mm

1/case

719808

Stainless Steel Fixed Probe*

60 mm

8/case

719544

Stainless Steel Fixed Probe*

100 mm

1/case

719810

Stainless Steel Fixed Probe*

100 mm

8/case

A34967

Teflon-Coated Fixed Probe

60 mm

1/case

A39379

Teflon-Coated Fixed Probe

60 mm

8/case

A34966

Teflon-Coated Fixed Probe

100 mm

1/case

A39377

Teflon-Coated Fixed Probe

100 mm

8/case

717931

Teflon-Coated Fixed Probe*

60 mm

1/case

719807

Teflon-Coated Fixed Probe*

60 mm

8/case

717933

Teflon-Coated Fixed Probe*

100 mm

1/case

719809

Teflon-Coated Fixed Probe*

100 mm

8/case

987873

Stainless Steel Piercing Probe*

120 mm

1/pack

987872

Stainless Steel Piercing Probe*

120 mm

4/pack

987870

Stainless Steel Piercing Probe*

120 mm

8/pack

A49675

Large Volume Tubing Kit†

* Requires Biomek Large Volume Tubing Kit (Part No. A49675). † The Biomek Large Volume Tubing Kit includes tubing and other parts necessary to convert a system with low-volume tubing to large-volume tubing for use with large-volume syringes or stainless steel piercing probes. 100 mm and 120 mm probes are compatible with Biomek NX P /FX P and i-Series Span-8 Pods. Teflon is a registered trademark of E.I. du Pont de Nemours and Company.

15

To order, visit beckman.com or contact your local representative.

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