AOAC SPDS Mid Year 2016
Commercial Collagen Products
1. Gelatin : • an irreversibly denatured (Heat or Acid) form of collagen (usually types I & III) used in food and cosmetics industry
2. Partially denatured (physical breakdown) or non-hydrolyzed : • All types (I/III and II are most common) • High molecular weight • Limited water solubility (soluble in mildly acidic solutions)
3 Hydrolyzed . • Type I/III (derived from beef, pig or fish skin and bones) • Type II (usually from chicken sternal cartilage), can be
SMPR Key Points
Applicability : The method will be able to identify and quantify individual native (un‐ denatured) and hydrolyzed collagen type I, II & III if one or multiple types are present in dietary ingredients and dietary supplement finished products.
Validation Guidance : Data demonstrating that a candidate method is able to: Separate a bi i f i ll com nat on o nat ve co agen type I, II and III and/or hydrolyzed collagen type I, II and III. Quantify each individual collagen type both native and hydrolyzed.
Parameter Criteria Table 1: Method performance requirements Analytical Range (%) 1 – 100 LOQ (%) 0.5 Recovery (%) 90‐110 % RSD r ≤ 5 % RSD R ≤ 10
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