AOAC SPDS Mid Year 2016

4. 3’-Epilutein

o (3R,3’S,6’R)‐Lutein o Not dietary— no biological or commercial role o Significant epimer product and loss of lutein o Occurs in aqueous acid o Reaction likely proceeds by S N 1 and S N 2, but mostly S N

2 since 

conversion exceeds 50% 

o Structure

General Analytical Needs

Method should – Quantitatively de‐esterify all analyte forms – Separate and accurately quantify relevant free  analytes • Lutein • Zeaxanthin • β‐Cryptoxanthin 3’ E il t i ( i i l l t i t b lit ) • ‐ p u e n pr nc pa u e n me a o e – Determine the above in • Raw materials used in dietary supplement  formulations • Finished products