AOAC RI ERP E-Book - DS DF

AOAC RESEARCH INSTITUTE AOAC OFFICIAL METHODS OF ANALYSIS (OMA)

OMAMAN-13: Determination Of Dietary Starch In Animal Feeds And Pet Food By An Enzymatic- Colorimetric Method Collaborative Study* Study Director: Mary Beth Hall, U. S. Department of Agriculture – Agricultural Research Service, U.S. Dairy Forage Research Center, 1925 Linden Drive, Madison, WI 53706, USA

First Action Recommendation

ER 1

Yes

ER 2 Yes, I do recommend the method to be adopted as First Action Method by ERP after authors have explained the following two limitation of the method in application. 1. The method in the current format is not be easily applicable to foods/feeds high in phenolic compounds (e.g. beets, red sorghum grain). 2. Oats beta-glucan interfere in the assay and provide values above LOD = 0.31 +/- 0.09%. ER 3 Yes. Recommend consideration of allowing HPLC as an option to measure liberated glucose to calculate %dietary starch. This approach would also measure free, inherent glucose and track if sucrose-digestion has occurred. ER 4 Yes ER 5 Yes. ER 6 Yes ER 7 yes ER 8 Yes ER 3 Recommend consideration of allowing HPLC as an option to measure liberated glucose to calculate %dietary starch. This approach would also measure free, inherent glucose and track if sucrose- digestion has occurred. Decision needed if single or multiple lab work is needed to verify. ER 4 Just the normal 2 year feedback period. Collaborative is completed and complete. ER 5 N/A ER 6 It would be good to test the performance of the method when an alternative glucose assay is used. Clarify the reason why dextrin recovery is low. ER 7 no ER 8 That included in the text above. (Please clarify) AOAC Research Institute ERP Use Only After First Action Recommendation ER 1 ER 2 Use feedback NA