AOAC ISPAM Stakeholder Panel Meeting Book 9-24-17

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• determine and submit estimates for recovery for each gluten source (wheat, rye, and barley) using the oat flour testing materials (see reference materials section for

source information).

For all claimed matrices, developers must:

• submit a sample processing procedure for homogenization, particle size reduction,

and test portion size.

• submit data and estimates for LOD and LOQ. • submit precision estimates (RSD r and/or RSD R )

100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117

Method developers may use spiked samples to determine the recovery performance of candidate methods for specific claimed matrices. Guidance on spiking for recovery

evaluation are provided at:

• Koerner et al.: Journal of AOAC INTERNATIONAL Vol. 96, no. 5, 2013 (see section on

“Spiking Methods”.

• Estimating Recovery of a Gluten ELISA Kit Method .

Appendix D : Guidelines for Collaborative Study Procedures To Validate Characteristics of a Method of Analysis; 20 th Edition of the AOAC INTERNATIONAL Official Methods of Analysis

(2016). Available at: http://www.eoma.aoac.org/app_d.pdf

Appendix F : Guidelines for Standard Method Performance Requirements; 20 th Edition of the AOAC INTERNATIONAL Official Methods of Analysis (2016). Available at:

http://www.eoma.aoac.org/app_f.pdf