AOAC ISPAM Stakeholder Panel Meeting Book 9-24-17

Challenges ƒ In the past, gluten methods were evaluated for accuracy based on spiking wheat gluten into various gluten-free matrices, and calculating “recovery” by the usual means of estimating the percentage of the analyte recovered from the collaboratively studied samples. ƒ Recent quantitative methods, such as AOAC OMA methods 2012.01 and 2014.03 have used this method. In the case of validating an ELISA method for gluten in oats, however, it will be essential to evaluate the kit responses to not only wheat, but also barley and rye. ƒ We are outlining a proposed procedure to evaluate kit response to all 3 contaminant grains independently. Challenges ƒ In 2010 the AOAC Allergen Community published a paper in the JAOAC outlining best practices for validating qualitative Allergen ELISA methods by Abbot et al., (Abbot, M., Hayward, S., Ross, H., Godefroy, S., Ulberth, F., Hengel, A., Roberts, J., Akiyama, H., Popping, B., Yeung, P., Wehling, P., Taylor, S., Poms, R., and Delahaut, P. J. AOAC Int., 274 93, (2010). pp. 442-450, ƒ In 2013 another paper for validating quantitative gluten ELISA methods by Koerner et al., (Validation Procedures for Quantitative Gluten ELISA Methods: AOAC Allergen Community Guidance and Best Practices, Terry B. Koerner, Michael Abbott, Samuel Godefroy, Bert Popping, Jupiter Yeung, Carmen Diaz-Amigo, James Roberts, Steve L. Taylor, Joseph L. Baumert, Franz Ulberth, Paul Wehling, Peter Koehler, J. AOAC Int., 96, 280 (2013), 1033 – 1040)