AOACRIMicroMethods-2017Awards
434 C rowley et al . : J ournal of AOAC I nternational V ol . 97, N o . 2, 2014
Table 2013.10A. Summary of results for the detection of Listeria spp. in queso fresco (25 g) Method a VIDAS LPT w/OXA
VIDAS LPT w/ALOA
Inoculation level
Uninoculated
Low
High Uninoculated
Low
High
Candidate presumptive positive/total No. samples analyzed
1/156
80/156
156/156
1/156
80/156
156/156
Candidate presumptive POD (CP)
0.01
0.51
1.00
0.01
0.51
1.00
(0.01, 0.04)
(0.43, 0.59)
(0.98, 1.00)
(0.01, 0.04) (0.43, 0.59)
(0.98, 1.00)
b
s r
0.08
0.51
0.00
0.08
0.51
0.00
(0.07, 0.15)
(0.46, 0.52)
(0.00, 0.15)
(0.07, 0.15) (0.46, 0.52)
(0.00, 0.15)
c
0.00
0.00
0.00
0.00
0.00
0.00
s L
(0.00, 0.03)
(0.00, 0.13)
(0.00, 0.15)
(0.00, 0.03) (0.00, 0.13)
(0.00, 0.15)
d
0.08
0.51
0.00
0.08
0.51
0.00
s R
(0.07, 0.13)
(0.46, 0.52)
(0.00, 0.21)
(0.07, 0.13) (0.46, 0.52)
(0.00, 0.21)
P value e
0.4395
0.9210
1.0000
0.4395
0.9210
1.0000
Candidate confirmed positive/total No. samples analyzed 0/156
78/156
156/156
0/156
78/156
156/156
Candidate confirmed POD (CC)
0.00
0.50
1.00
0.00
0.50
1.00
(0.00, 0.02)
(0.42, 0.58)
(0.98, 1.00)
(0.00, 0.02) (0.42, 0.58)
(0.98, 1.00)
s r
0.00
0.51
0.00
0.00
0.51
0.00
(0.00, 0.15)
(0.46, 0.52)
(0.00, 0.15)
(0.00, 0.15) (0.46, 0.52)
(0.00, 0.15)
0.00
0.00
0.00
0.00
0.00
0.00
s L
(0.00, 0.15)
(0.00, 0.14)
(0.00, 0.15)
(0.00, 0.15) (0.00, 0.14)
(0.00, 0.15)
0.00
0.51
0.00
0.00
0.51
0.00
s R
(0.00, 0.21)
(0.46, 0.52)
(0.00, 0.21)
(0.00, 0.21) (0.46, 0.52)
(0.00, 0.21)
P value
1.0000
0.9161 76/156
1.0000 156/156
1.0000
0.9161 76/156
1.0000 156/156
Positive reference samples/total No. samples analyzed
0/156
0/156
Reference POD
0.00
0.49
1.00
0.00
0.49
1.00
(0.00, 0.02)
(0.41, 0.57)
(0.98, 1.00)
(0.00, 0.02) (0.41, 0.57)
(0.98, 1.00)
0.00
0.52
0.00
0.00
0.52
0.00
s r
(0.00, 0.15)
(0.46, 0.52)
(0.00, 0.15)
(0.00, 0.15) (0.46, 0.52)
(0.00, 0.15)
0.00
0.00
0.00
0.00
0.00
0.00
s L
(0.00, 0.15)
(0.00, 0.10)
(0.00, 0.15)
(0.00, 0.15) (0.00, 0.10)
(0.00, 0.15)
0.00
0.52
0.00
0.00
0.52
0.00
s R
(0.00, 0.21)
(0.47, 0.52)
(0.00, 0.21)
(0.00, 0.21) (0.47, 0.52)
(0.00, 0.21)
P value
1.0000
0.9937
1.0000
1.0000
0.9937
1.0000
dLPOD (candidate vs reference)
0.00
0.01
0.00
0.00
0.01
0.00
(–0.02, 0.02) (–0.10, 0.13)
(–0.02, 0.02) (–0.02, 0.02) (–0.10, 0.13) (–0.02, 0.02)
dLPOD (candidate presumptive vs candidate confirmed)
0.01
0.01
0.00
0.01
0.01
0.00
(–0.02, 0.04) (–0.10, 0.13)
(–0.02, 0.02) (–0.02, 0.04) (–0.10, 0.13) (–0.02, 0.02)
a Results include 95% confidence intervals. b Repeatability standard deviation. c Among-laboratory standard deviation. d Reproducibility standard deviation. e P value = Homogeneity test of laboratory PODs.
handled observing the usual safety precautions (do not ingest or inhale).
the enzyme-linked fluorescent assay (ELFA) method. The assay also incorporates phage proteins allowing an increase in sensitivity and specificity compared to traditional immunoassay. The Solid Phase Receptacle (SPR ® ) serves as the solid phase as well as the pipetting device. The interior of the SPR is coated with proteins specific for Listeria receptors. Reagents for the assay are ready-to-use and predispensed in the sealed reagent
A. Principle VIDAS ® UP Listeria (LPT) method is for use on the automated VIDAS instrument for the detection of Listeria antigens using
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