September 2016 SPDS Book
Existing Methods
HPLC WITH PRECOLUMN DERIVATIZATION
► Method
Reversed phase or silica column
o-phthaldehyde (OPA), phenylisothiocyanate (PITC), 9- fluorenylmethyl chloroformate (F-MOC) Fluorescence or UV detection ► Advantages Derivatization conditions (reagent and detector) can be optimized for matrix and analytes of interest – Greater sensitivity Allows for using rapid reversed-phase chromatography
11
Existing Methods
HPLC WITH POSTCOLUMN DERIVATIZATION (AOAC METHOD)
► Method
Cation-exchange chromatography Ninhydrin, fluorescamine, OPA Fluorescence or UV detection
► Advantages
Can be automated resulting in increased performance and reproducibility Reaction efficiency less prone to sample matrix effects Minimal sample preparation
12
6
Made with FlippingBook