September 2016 SPDS Book
Existing Methods
► Gas Chromatography Precolumn derivatization to less polar and more volatile OTHER METHODS compounds FID or MS detection ► Capillary Electrophoresis Precolumn, postcolumn, and oncolumn derivatizations Fluorescence or UV detection ► HPAEC-PAD D i ti ti t i d er va za on no requ re ► HPLC-MS/MS Derivatization not required Mainly clinical applications to date
13
Challenges
► Multiple analytes with varying functional groups make utilizing one method difficult ► HPLC with Precolumn Derivatization Sample matrix can effect reaction efficiency Reaction products can be unstable ► HPLC with Postcolumn Derivatization
High reagent consumption Limited variety of reagents L ti ( 60 i ) ong run me ~ m n Potential for loss of resolution Less sensitive
14
7
Made with FlippingBook