September 2016 SPDS Book

Existing Methods

► Gas Chromatography  Precolumn derivatization to less polar and more volatile OTHER METHODS compounds  FID or MS detection ► Capillary Electrophoresis  Precolumn, postcolumn, and oncolumn derivatizations  Fluorescence or UV detection ► HPAEC-PAD D i ti ti t i d  er va za on no requ re ► HPLC-MS/MS  Derivatization not required  Mainly clinical applications to date

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Challenges

► Multiple analytes with varying functional groups make utilizing one method difficult ► HPLC with Precolumn Derivatization  Sample matrix can effect reaction efficiency  Reaction products can be unstable ► HPLC with Postcolumn Derivatization

 High reagent consumption  Limited variety of reagents L ti ( 60 i )  ong run me ~ m n  Potential for loss of resolution  Less sensitive

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