AOAC-03 Preliminary Program

This session will discuss various approaches for the implementation of LC-MS/MS methods for mycotoxin analysis in food and feed. This session will focus on multi- analyte method development and will give some insight into the regulations and changes during recent years. CO-CHAIR: Joerg Stroka, JRC-Geel CO-CHAIR: Lilian Kuster, Romer Labs Division Holding GmbH • Jon Wong, U.S. Food and Drug Administration Perspective on Advancing FDA Regulatory Monitoring for Mycotoxins in Food Using Liquid Chromatography and Mass Spectrometry • Alois Schiessl, Romer Labs Division Holding GmbH LC-MS/MS Multi Mycotoxin Developments in Routine Labs • Michael Sulyok, University of Natural Resources and Life Sciences, Vienna Analytical Performance of an LC-MS/MS Based “Dilute and Shoot” Approach for Multi-Mycotoxin Analysis • Joerg Stroka, JRC-Geel Method Performance for Mycotoxins: What Can We Conclude from the Last Decade? • Tetsuhisa Goto, Retired from Shinshu University Mycotoxin Regulation in Pet Food and Feed in Japan Food Allergen and Gluten Analysis by LC-MS: Where Are We? Food allergies and intolerance to gluten are important health concerns worldwide with the only prescription being the complete avoidance of the specific food proteins in order to eliminate the risk of an adverse reaction. Analytical methods are a key component in allergen control programs and in the enforcement of regulatory requirements. Immunological based methods such as enzyme-linked immunosorbent assays (ELISA) are important tools for the detection and quantification of food allergens, but due to limitations with some matrices and processing methods (e.g. hydrolyzed and fermented foods) there is a need for alternative confirmatory methods of analysis. Mass spectrometry (MS) methods coupled to liquid chromatography (LC) are being developed that directly measure selected marker peptides from food allergens. These tandem mass spectrometry experiments (LC-MS/MS) have the potential for both qualitative and quantitative determination of multiple food allergens in one analysis with a specificity that cannot be achieved by antibodies. This session will provide an update on the status of these promising mass spectrometry methods from different international laboratories using an assortment of different instrument technologies.

of dietary supplement (and foods), botanical suppliers dealing with FSMA requirements, and regulatory experts. The discussion is expected to be free-wheeling and will range from identity testing requirements, “reasonably anticipated contaminants”, standard operating procedures, “verification or validation”, and qualification of standards and reference materials. The ultimate goal of the session is to put manufacturers, analytical test methods / analysts, contract labs and regulatory officials in the same room so that fruitful discussions can take place involving real time challenges / issues and recommendations of solutions to curtail them. CO-CHAIR: Amitabh Chandra, AMWAY CO-CHAIR: Joseph Betz, National Institutes of Health • Presenters TBD Mycotoxin News—Ready for the Future Mycotoxin contamination in food and feed poses a serious threat for human and animal health. For this reason, numerous countries have introduced regulatory limits. Highly precise analytical methods are needed to enable reliable controls of the maximum limits of these compounds. In recent decades, mass spectrometry based methods like LC-MS/MS have gained importance and can now be considered as the most accurate tools for mycotoxin analysis. Due to the high sensitivity, selectivity, and accuracy of these methods, as well as the increased availability of isotope labeled internal standards, a wide range of analytical methods using LC-MS/MS are rapidly being developed. These new approaches are replacing more traditional methods such as HPLC or TLC. Innovative isotopic labeled internal standards in combination with efficient clean-up prior to LC-MS/MS analysis make it possible to overcome matrix effects and ion enhancement/ suppression effects. In addition, a variety of mycotoxins can now be analyzed simultaneously using LC-MS/MS multi-analyte methods. WEDNESDAY, SEPTEMBER 21, 2016 8:15 am – 9:45 am

CO-CHAIR: Jerry Zweigenbaum, Agilent Technologies, Inc. CO-CHAIR: Terry Koerner, Health Canada

21

www.aoac.org

301.924.7077