AOAC SPIFAN Stakeholder Program Meeting Book (March 17, 2021)

Beta-casein Under Gut Digestion

58

54

55

Val

Tyr

Pro Phe Val

Glu Pro Ile Pro

Asn

Ser

Leu Pro

Human beta-casein

Val

Tyr

Pro Phe Pro Gly Pro Ile Pro

Ser

Leu Pro

Asn

Bovine beta-casein A2

Val

Tyr

Pro Phe Pro Gly Pro Ile

His

Asn Ser Leu Pro

Bovine beta-casein A1

67

Pro

Pro Asn

Tyr Pro Phe

Ile

Pro Gly

A2 release BCM-9

Amino Acid position 67

digestion

A2 beta casein protein chain (209AA) A1 beta casein protein chain (209AA)

Val

Tyr

Pro Phe Pro Gly Pro Ile Pro

Ser

Leu Pro

Asn

Val

Tyr

Pro Phe Pro Gly Pro Ile

His

Asn Ser Leu Pro

Amino Acid position 67

digestion

Zinnia Zhang, Kevin Mahan, Abbott internal poster adapted from Gobbetti M. et al. 2002 CritRev Food SciNutr.;42(3):223-39. Jinsmaa, Y & Yoshikawa, M. 1999. Peptides V20(8), pp 957-962.

A1 releases BCM-7 Reported as strong exorphin

Tyr

Gly Pro Ile

Pro Phe Pro

Beta-Casein Genotypes

X Eg. Frequency of cow breeds

HOLSTEIN A2A2 A1A2 A1A1

JERSEY A2A2 A1A2 A1A1

3%

16%

35%

32%

65%

49%

6

Lynsay Beavers L., Van Doormaal B. Canadian Dairy Network