SPSFAM_Sugar_ERP-92018Proposal

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Graduate Research Assistant Department of Chemistry, Purdue University, West Lafayette, IN 2011-2015 • Constructed and characterized custom ion trap based mass spectrometric instrumentation to analyze high resolution, cold UV/Vis and IR spectroscopy of biologically relevant ions • Analyzed and interpreted mass spectrometric data, spectroscopic data, and theoretical predictions for conformation-specific, three-dimensional structural characterization of ions • Acquired, analyzed, and interpreted mass spectrometric data on a variety of custom and commercial instrument platforms, including ion trap and Time-of-Flight instruments • Preformed extensive troubleshooting of instrumentation (custom and commercial) and analytical methodologies • Coordinated multidisciplinary project management with machinists, engineers, and chemists for custom instrumentation development, construction and support Undergraduate Research Assistant Department of Chemistry, University of North Carolina, Chapel Hill, NC 2009-2010 • Designed and manufactured glass micro-fluidic devices for capillary electrophoresis (CE) separations via photolithography • Optimized micro-fluidic channel designs, polymer coatings and solvent systems for manipulation of electrophoretic and electroosmotic flow, improving the efficiency of capillary electrophoresis separations • Conducted analysis of protein separations via CE-electrospray ionization mass spectrometry in micro-fluidic chips, leading to an improved understanding of structural proteomics TECHNICAL SKILLS Instrumentation: Confocal Raman microscope (Renishaw inVia), FT NIR spectrometers (Bruker MPA), dispersive NIR spectrometers (Unity, Unity XL, Unity XT), UV-Vis spectrometers, UV-Vis-NIR spectrometers, HPLC (Shimadzu LC- 10, Agilent 1100, Agilent 1200), UPLC (Agilent 1290 Infinity, Waters Acquity I-Class), Ion Chromatography, triple quadrupole mass spectrometer (MS) (Waters TQ-S Micro), quadrupole time-of-flight (TOF) MS (Agilent 6530 Accurate- Mass Q-TOF), custom-built MS for cold ion spectroscopy (ICIS@Purdue), custom-built RF supplies, modified QqQ geometry linear ion trap (LIT) MS (Sciex QTRAP 2000 & 4000), Modified QqTOF geometry LIT MS (AB Sciex QSTAR), Q-Switched Nd:YAG lasers (Continuum Surelite II & EX, Quantel Brilliant B), Tunable dye lasers (Lambda Physik ScanMate & ScanMatePro), Tunable solid state lasers (Continuum Horizon, Laservision OPO/OPA), Ultra-high vacuum and cryogenic (4K) system construction/maintenance, Griffin Analytical Technologies Argos Controller (FLIR) Software : Microsoft Office Suite, LIMS, Agilent Masshunter, Thermo Scientific Chromeleon, Waters MassLynx, Sciex Analyst, Unscrambler, GRAMs, Pirouette, OPUS, UCal, MathCAD, Igor, Sciex PeakView, Gaussian, Q-Chem, Gromacs, TINKER, Macromodel, Agilent Intuilink, Argos Control, LabVIEW, Fortran90, Adobe Dreamweaver CC PUBLICATIONS 7. Andrew F. DeBlase, Christopher P. Harrilal, John T. Tawler, Nicole L. Burke , Scott A. McLuckey, Timothy S. Zwier. J ournal of the American Chemical Society , 2017 , 139 , 5481-5493. "Conformation-Specific Infrared and Ultraviolet Spectroscopy of Cold {YAPAA+H]+ and [YGPAA+H]+ Ions: A Stereochemical "Twist" on the β-Hairpin Turn." 6. Andrew F. Deblase, Eric T. Dziekonski, John R. Hopkins, Nicole L. Burke , Huaming Sheng, Hilkka I. Kenttamaa, Scott A. McLuckey, Timothy S. Zwier, Journal of Physical Chemistry A , 2016 , 120, 7152-7166. “Alkali Cation Chelation in Cold β-O-4 Tetralignol Complexes.” 5. Nicole L. Burke , Andrew F. DeBlase, James G. Redwine, John R. Hopkins, Scott A. McLuckey, Timothy S. Zwier, Journal of the American Chemical Society , 2016 , 138, 2849-2857. “Gas-Phase Folding of a Prototypical Protonated Pentapeptide: Spectroscopic Evidence for Formation of a Charge-stabilized β-Hairpin.” 4. Nicole L. Burke , James G. Redwine, Jacob C. Dean, Scott A. McLuckey, Timothy S. Zwier, International Journal of Mass Spectrometry , 2015 , 378, 196-205. “UV and IR spectroscopy of cold protonated leucine enkephalin” 3. Jacob C. Dean, Nicole L. Burke , John R. Hopkins, James G. Redwine, P. Veeraraghavan Ramachandran, Scott A. McLuckey, Timothy S. Zwier, Journal of Physical Chemistry A , 2015 , 119, 1917-1932. “UV photofragmentation and IR spectroscopy of cold, G-type β-O-4 and β-β dilignol-alkali metal complexes: structure and linkage-dependent photofragmentation.”

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