2018 August 26 SPSFAM Book

Challenging Issues

Sample preparation (extraction and clean-up) • Is there an extraction procedure that can extract the target mycotoxins from a variety of matrices? LC-MS (LC-MS/MS and LC-HRMS) • What would be a practical approach to handle matrix-effects? Validation of multi-mycotoxin methods

• How to set up method performance criteria? Implementation of multi-mycotoxin methods • How to verify/extend validated methods?

Important Method Performance Criteria

AOAC Guidelines for Standard Method Performance Requirements ( http://www.eoma.aoac.org/app_f.pdf )

Guidelines for the Validation for Chemical Methods for the FDA FVM Program (2 nd ) ( https://www.fda.gov/downloads/scienceresearch/fieldscience/ucm273418.pdf ) SANTE/11813/2017. Guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed. ( https://ec.europa.eu/food/sites/food/files/plant/docs/pesticides_mrl_guidelines_wrkdoc_2017-11813.pdf )