RI-ERP-FINALACTION-Recommendations

OMA 2015.13: Collaborative Study Protocol Expert Review Panel Use Only September, 2017

Appendix 8.3

Study Materials Materials and reagents provided by the study sponsor 1. 3MPetrifilmFlat Spreader (6425) 2. 3MPetrifilmRapid Aerobic Count Plates 3. Sterile diluent –Butterfields Phosphate Diluent 4. Plate Count Agar/Standard Methods Agar 5. 3M Petrifilm Aerobic Count Plates

Materials required but not provided

1. Pipettes . –capable of pipetting 1,000µL or sterile serological pipette 2. Sterile pipette tips. –capable of 1,000µL 3. Stomacher® . –Seward or equivalent 4. Filter Stomacher®bags . - Seward or equivalent

5. Incubators . –Incubator capable of maintaining 32±1°C and another capable of maintaining 35±1°C, and having a solid front so as to maintain a dark interior. 6. Refrigerator . –capable of maintaining 2-8°C, for storing the 3M Petrifilm RAC Plates 7. Freezer . - capable of maintaining -10° to -20°C, for storing opened 3M Petrifilm RAC Plate pouches, 3M Petrifilm RAC Plates after incubation

AOAC Research Institute Expert Review Panel Use Only