SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 05 lot 20

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S1-Rep3

Vial No

: 10002

Injection Volume

: 20

Date & Time

: 4/4/07 11:01:48 AM

0.15

22.067

0.10

Volts

17.225

23.908

23.042

0.05

21.408

27.025

0.00

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.408 22.067 23.042 23.908 27.025

Withanoside IV Withanoside V Withaferin A

292499 182887 928052 304876 323917

13.99

8.75

44.39 14.58 15.49

Withastramonolide

Withanolide A Withanolide B

58492

2.80

Totals

2090723

100.00