AOAC-RI ERP Micro June 2016

OMAMAN-29 A/ Collaboartive Study Manuscript OMA ERP June 2016 ERP Use Only

AOAC Official Method 2016.xxx

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Listeria speciesin SelectFoods and Environmental Surfaces 3M ™ Molecular Detection Assay(MDA) 2- Listeria Method

First Action 2016 4 5 (Applicable to detection of Listeria speciesin hot dogs (25g & 125g), salmon (25g), deli turkey 6 (25g & 125g), cottage cheese (25g), vanilla ice cream (25g), queso fresco (25g), spinach (25g), 7 melon (whole), raw chicken leg pieces (25g), raw chicken fillet (25g); concrete (3M™ Hydrated 8 Sponge Stick with D/E, 225 mL & 100 mL), stainless steel (3M™ Hydrated Sponge Stick with 9 D/E, 225 mL), and plastic (3M™ Enviroswab with Letheen, 10 mL) environmental samples. 10 11 See Tables 2016.1A and 2016.1B for a summary of results of the inter-laboratory study. 12 See Tables 2016.2A and 2016.2B for detailed results of the inter-laboratory study 15 16 The 3M ™ Molecular Detection Assay (MDA) 2 - Listeria method is used with the 3M ™ 17 Molecular Detection System (MDS) for the rapid and specific detection of Listeria in enriched 18 food and food process environmental samples. The 3M MDA2 - Listeria uses loop-mediated 19 isothermal amplification of unique DNA target sequences with high specificity and sensitivity, 20 combined with bioluminescence to detect the amplification. Presumptive positive results are 21 reported in real-time while negative results are displayed after the assay is completed. Samples 22 arepre-enriched in Demi Fraser broth with ferric ammonium citrate (FAC). 13 14 A. Principle

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B. Apparatus and Reagents

Items (b)-(g) are available as the 3M ™ Molecular Detection Assay (MDA) 2 - Listeria kit

from 3M Food Safety (St. Paul, MN 55144-1000, USA).

(a) 3M Molecular Detection System (MDS100) – Available from 3M Food Safety (St.

Paul, MN 55144-1000, USA).

(b) 3M Molecular Detection Assay 2 – Listeria reagent tubes- 12 strips of 8 tubes. Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).

(c) Lysis Solution (LS) tubes – 12 strips of 8 tubes.

(d) Extra caps – 12 strips of 8 caps (e) Reagent Control – 8 reagent tubes

(f) Quick Start Guide (St. Paul, MN 55144-1000, USA). AOAC Research Institute Expert Review Panel Use Only (h) 3M ™ Molecular Detection Chill Block Insert - Available from 3M ™ Food Safety (St. Paul, MN 55144-1000, USA). (i) 3M ™ Molecular Detection Heat Block Insert - Available from 3M Food Safety (g) 3M ™ Molecular Detection Speed Loader Tray - Available from 3M Food Safety

(St. Paul, MN 55144-1000, USA).

(j) 3M ™ Molecular Detection Cap/Decap Tool for Reagent tubes - Available from 3M

Food Safety (St. Paul, MN 55144-1000, USA).

(k) 3M ™ Molecular Detection Cap/Decap Tool for Lysis tubes – Available from 3M

Food Safety (St. Paul, MN 55144-1000, USA).

(l) Empty Lysis Tube Rack - Available from 3M Food Safety (St. Paul, MN 55144-1000,

USA).

(m) Empty Reagent Tube Rack - Available from 3M Food Safety (St. Paul, MN 55144-

1000, USA).

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