AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

1 2 3 4 5 6 7 8 9

procedure. There were 3 observed positives for the reference method. For the high inoculation level, there were 3 presumptive positives and 3 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 4 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 8 and 12. This matrix analysis was conducted using Demi Fraser with FAC brand X.

Compared to ISO 11290-1/A1

For the low inoculation level of the MDA 2 - Listeria assay, there were 6 presumptive positives and 6 confirmed positives following the ISO 11290-1/A1 reference method confirmation procedure. There were 9 observed positives for the reference method. For the high inoculation level, there were 3 presumptive positives and 3 confirmed positives following the ISO 11290- 1/A1 reference method confirmation procedure. There were 4 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 8 and 12 This matrix analysis was conducted using Demi Fraser with FAC brand X.

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46

Cold smoked salmon

Compared to FDA-BAM

For the low inoculation level of the MDA 2 - Listeria assay, there were 10 presumptive positives and 10 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 7 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 4 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 8 and 12. This matrix analysis was conducted using Demi Fraser with FAC brand X.

Compared to ISO 11290-1/A1

For the low inoculation level of the MDA 2 - Listeria assay, there were 10 presumptive positives and 10 confirmed positives following the ISO 11290-1/A1 reference method confirmation procedure. There were 8 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the ISO 11290- 1/A1 reference method confirmation procedure. There were 4 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 8 and 12. This matrix analysis was conducted using Demi Fraser with FAC brand X.

AOAC Research Institute Expert Review Panel Use Only

Independent Study using Demi Fraser with FAC brand Y.

Deli turkey (125 g)

For the low inoculation level of the MDA 2 - Listeria assay, there were 8 presumptive positives and 8 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation