2018 - March 12 SPSFAM Meeting Book

Background: Sugar

• For animal feeds, current sugar analyses typically rely on extraction, acid  hydrolysis of solubilized carbohydrates, and measurement as reducing sugars,  or measurement of total extracted carbohydrates using condensation  reactions. – Inappropriately includes oligosaccharides and other solubilized  carbohydrate in the sugar measurement.  Also for some feeds, active  amylases digest carbs into sugars thus artificially increasing the measured  sugar amount. • For measuring individual sugars in foods, traditional HPLC methods have  difficulty – separating some monosaccharides from each other, and – resolving sugars from interfering compounds such as sugar alcohols

Background: Sugar

• Currently there are no approved methods for individually measuring mono- and di-saccharides in animal feed or pet food. • RI methods do not achieve desired level for low level sugar guarantees. • AOAC Official Method 974.06 Sugars (Total) in Animal Feed Modified Fehling Solution Method • Several issues on accounting for different sugars being present