2018 - March 12 SPSFAM Meeting Book

Stakeholder Panel for STRATEGIC FOOD ANALYTICAL METHODS (SPSFAM)

MONDAY, MARCH 12, 2018 GAITHERSBURG MARRIOTT WASHINGTONIAN CENTER 9571 Washingtonian Blvd, Gaithersburg, MD, 20878 CONFERENCE ROOM: Salon C/D/E

1:00pm – 5:00pm Eastern Time Registration Opens at 12:00Pm

CONTACT: SPSFAM@AOAC.ORG

Stakeholder Panel for STRATEGIC FOOD ANALYTICAL METHODS (SPSFAM)

MONDAY, MARCH 12, 2018 GAITHERSBURG MARRIOTT WASHINGTONIAN CENTER 9571 Washingtonian Blvd, Gaithersburg, MD, 20878 CONFERENCE ROOM: Salon C/D/E

1:00pm – 5:00pm Eastern Time Registration Opens at 12:00Pm

CONTACT: SPSFAM@AOAC.ORG

Stakeholder Panel on Strategic Food Analytical Methods - Chair Biography

Co‐Chair, SPSFAM Erik Konings Nestle Research Center Erik Konings studied higher professional laboratory education with majors in analytical and clinical chemistry. After graduating in 1984, he started his professional career at the then called Food Inspection Service in Maastricht, the Netherlands. In 2001 he completed his PhD study “Dietary folates in human nutrition” at Maastricht University. During this study, he obtained an MSc-degree in epidemiology. He is (co)author of more than 30 scientific publications. In September 2008 he started at the European Food Safety Authority (EFSA) in Parma, Italy, for a secondment as Scientific Officer at the Data Collection and Exposure Unit, and from there accepted, in June 2009, a position at the Nestlé Research Centre in Lausanne, Switzerland, currently in a role as Food Safety & Quality expert. He is active in several Standard Developing Organisations as AOAC INTERNATIONAL (Past-President), ISO, CEN, and IDF, and participates in the Codex Committee on Methods of Analysis and Sampling (CCMAS).

PRESENTER BIOS

SUSAN AUDINO, Audino & Associates, LLC Chair, Cannabis Working Group

Susan Audino obtained her PhD in Chemistry with an analytical chemistry major, physical and biochemistry minor areas. Susan was the recipient of NSF Chemometric Graduate Fellowship and was a visiting scientist at NIST where she completed her graduate research. She currently owns and operates a consulting firm to service chemical and biological laboratories, is an A2LA Lead Assessor and Instructor, and serves as a Board Member for the Center for Research on Environmental Medicine in Maryland. She is also serving as Quality Director for several laboratories and has worked with a

variety of laboratories to establish and/or improve their quality management systems.

Susan has been studying the chemistry and applications of cannabinoids and provides scientific and technical guidance to medical marijuana dispensaries, testing laboratories, medical personnel, and regulatory agencies. Dr. Audino’s interest most directly involves marijuana/cannabis consumer safety and protection, and promotes active research towards the development of Official Test methods specifically for this industry. In addition to serving on Expert Review Panels, she has been working closely with AOAC to develop interest and movement toward the development of scientifically sound methodologies for the cannabis sector. Prior to her study of chemistry, Dr. Audino received advanced degrees and practiced psychology for more than a decade.

JOE BOISON, Independent Consultant Chair, Veterinary Drugs Working Group

Dr. Boison recently retired from the Canadian Public Service after 31 years of public service. He still holds 2 Adjunct Professor Faculty positions in the College of Graduate Studies at the University of Saskatchewan. In 2003, he was appointed a Fellow of the World Innovation Foundation, was awarded the CFIA President’s National Award for Leadership Excellence in 2010, and in 2012 he was appointed a fellow of the AOACI. In 2017, he was appointed a senior member of the Joint FAO/WHO Expert Committee on Food Additives (JECFA); He was the winner of the 2015 AOAC Harvey W. Wiley Award, the recipient of the 2015 Expert Review Panel (ERP) Award for chairing the Pesticide Residues and Chemical Contaminants in Tea ERP and is currently chair of the AOAC gluten in oats initiative.

Dr. Boison is a member of the Spectroscopy Society of Canada, a member of the Standards and Measurement Committee for the American Society for Mass Spectrometry (ASMS), a member of AOAC INTERNATIONAL, member of the AOAC Editorial Board, the Scientific Editor for Veterinary Drugs, joint scientific editor for Drug Formulations and Clinical Methods for the Journal of AOAC International, Vice Chair of the AOAC Official Methods Board and Chair of the AOAC Research Institute Board of Directors.

As a consultant, Dr. Boison is regularly consulted within and outside government and the industry with regards to residue testing methods for in-plant and on-farm use. Dr. Boison’s research and academic interests during his tenure in the public service included development of chromatographic and mass spectrometric methods for the detection, identification and confirmation of veterinary drug residues in biological fluids and tissues in support of regulatory enforcement and/or for the pharmacokinetic and pharmacodynamic studies; development and adaptation of commercially available rapid tests for field and lab screening of drug residues in biological fluids and tissues; automation of laboratory methods for the analysis of veterinary and human drugs; and teaching/development of graduate and undergraduate students to acquire expertise in bio- analytical mass spectrometry, metabolism, and pharmacokinetic studies.

JOHN SZPYLKA, Mérieux NutriSciences Co-Chair, Sugars Working Group

Dr. John Szpylka is the Scientific Affairs Director, Chemistry N.A. with Mérieux NutriSciences where he manages nutritional analytical method development for Mérieux NutriSciences and is a technical leader for chemistry testing in North America. John is a representative to key scientific organizations and regulatory agencies to identify and contribute to food testing standardization for nutritional needs and arising issues. This includes active involvement in organizations including AOACI, AACCI, AOCS, AAFCO, ACIL, and DSQAP. John Szpylka is a Fellow of AOAC International and is a past chair of the AOAC Official Methods Board . He currently serves on numerous Stakeholder Panels and Expert Review

Panels. John also serves as a Board Member for the American Council of Independent Laboratories. Before joining Mérieux NutriSciences, John was a Principal Scientist with General Mills / Medallion Laboratories where he oversaw the development and operation of food analytical methods. John received his doctorate in analytical chemistry from the Ohio State University after receiving a B.S. in chemistry from Rensselaer Polytechnic Institute.

NANCY THIEX, Thiex Laboratory Solutions, LLC. Co-Chair, Sugars Working Group Thiex retired in 2011 after 35 years of service to South Dakota State University, where she managed an Analytical Services Laboratory. Since “retirement” Thiex has provided technical consulting as Thiex Laboratory Solutions LLC. She continues to be active in AAFCO and AOAC and other professional organizations, serving on many working groups, committees and task forces. Two of these volunteer efforts include co-chairing the AAFCO Laboratory Methods and Services Committee and serving as a section editor for JAOAC.

A significant work effort over the past five years has been contributing to the three-association cooperative agreement "Building an Integrated Laboratory System to Advance the Safety of Food and Animal Feed" funded by US FDA. In this effort lead by APHL, she serves as the AAFCO Lead Investigator and Project Manager. She is proud to have been able to contribute to the many valuable deliverables of this collaboration of FDA, APHL, AFDO and AAFCO.

SPSFAM Roster as of March 6, 2018 

SPSFAM Roster, March 6, 2018  This roster is also the SPSFAM Mailing List! Don’t see your name on the list? Please let us know by  emailing SPSFAM@aoac.org and we will add you right away to make sure you don’t miss any important  announcements!  Full Name  Position  Organization  Erik J. M. Konings  Chair  Nestle Research Center  Vanessa Abercrombie  Member  Agilent Technologies, Inc.  Luke K. Ackerman, PhD  Member  FDA/CFSAN  Claude Pierre Aebischer  Member  DSM Nutritional Products Ltd  David James Almy, M.S.  Member  Neogen Corporation  Mark Angerhofer  Member  TruSource  Patricia Atkins  Member  SPEX CertiPrep  Susan Audino  Member  Audino & Associates, LLC  Lauryn Bailey  Member  SCIEX  Charles A. Barber  Member  NIST  Brad Barrett  Member  Forrest L. Bayer  Member  Coca‐Cola Co.  Nizar Benismail  Member  Nestle Research Center  Phil Berrier  Member  The Coca‐Cola Company  Tim Beshore  Member  Chemours  Sneh D. Bhandari  Member  Merieux NutriSciences  Joe Boison  Member  Emma Bradley  Member  FERA  Hillel Brandes  Member  MilliporeSigma  Roger M. Brauninger  Member  A2LA  Michelle Briscoe  Member  Brooks Applied Labs  Paula N. Brown  Member  British Columbia Institute of Technology  Carolyn Quarles Burdette  Member  NIST  Nicole Burke  Member  Kellogg Company  Justin Burmeister  Member  Applied Technical Services, Inc.  Jeannie Buscher  Member  Buchi Kombucha  Anton Bzhelyansky  Member  US Pharmacopeia (USP)  Jim Cali  Member  Promega Corporation  Esther Campos‐Gimenez  Member  Nestle Research Center  Xu‐Liang Cao  Member  Health Canada  Evan Chaney  Member  Diamond V  Tony Chang  Member  International Chemistry Testing  Mr. Niladri Sekhar Chatterjee  Member  Indian Agricultural Research Institute  Mike Clark  Member  Bio‐Rad Laboratories

SPSFAM Roster as of March 6, 2018 

Robert Clifford.  Alessandra Conte  Claudio Cornaggia 

Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member 

Shimadzu 

Mérieux NutriSciences 

Megazyme 

Tim Croley 

US FDA ‐ CFSAN  Monster Energy 

Patrick Crook 

Erin Sutphin Crowley 

Q Laboratories, Inc. 

Hannah Crum 

Kombucha Brewers International 

David Cunningham 

Cunningham Consulting  Millennium Products, Inc.  Mérieux Nutriscience 

GT Dave 

Emiliano De Dominicis  Jonathan W. DeVries, Sr.  Carmen Diaz‐Amigo  Ms. Jennifer Donelson 

Retired 

FOCOS GbR 

Petroleum Analyzer Company (PAC) 

Jennifer Donelson  Robert Donofrio 

VUV Analytics 

Neogen Corporation 

Aurelie Dubois 

International Dairy Federation 

Lucien Duchateau  Blake E. Ebersole 

DSM Biotechnology  Verdure Sciences 

USDA ‐ FSIS ‐ Eastern Field Service  Laboratory 

Sarah Edwards  Stefan Ehling 

Abbott Nutrition 

Katherine M. Evans  Katherine Fiedler 

Patriot Labs 

U.S. FDA 

Roland Franz 

Fraunhofer 

Christophe Fuerer 

Nestle Research Center 

Jodie Fung 

Kombucha Brewers International 

Andrew Fussell  Eric A.E. Garber  Ed George, III  Kevin George  Russell Gerads  Peter Gibson  Brendon D. Gill  Michael Goodrich  Ms. Qian F. Graves  Keith Griswold, MA  Jasmine Hagan, B.S.  Cathy A. Halverson  Heather L. Harris, J.D.  Liwei Gu 

PANalytical 

US FDA 

Eurofins 

Brooks Rand Labs, LLC 

GW Research Ltd 

Fonterra Co‐operative Group Ltd. 

Cornerstone Labs 

FDA ‐ CFSAN 

Pepsico 

USDA ARS 

ELISA Technologies, Inc. 

TTB 

Nicole Hart 

Agilent Technologies 

Mr. Thomas Hartlein 

Teledyne Tekmar

SPSFAM Roster as of March 6, 2018 

Kevin Hegarty  Norma R. Hill  George Hodgin 

Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member 

Effendorf 

US Treasury (Retired) 

Biopharmaceutical Research Company 

Douglas Lloyd Holt  Gregory L. Hostetler 

Dr Pepper Snapple Group  Perrigo / PBM Nutritionals 

Amy Howell  Min Huang  Ronald Hunter 

Rutgers University 

Frontage Laboratories, Inc. 

Katherine C. Hyland  Dorota Inerowicz 

SCIEX 

Office Of The Indiana State Chemist 

Prashant Ingle 

Maia Jack 

American Beverage Association 

Brian P. Jackson  Greg Jaudzems 

Dartmouth College 

Nestle USA, Inc 

National Institute Of Technology  Rourkela 

Rasu Jayabalan  Holly E. Johnson  Richard S. Jordan  George Joseph  David C. Kennedy 

Alkemist Labs 

Pacific Agricultural Laboratory  AsureQuality, New Zealand 

Phenomenex 

Sam Khoury  Soline Kintz 

Cott Beverages, Inc.  Red Bull North America  National Food Agency 

Barbro Inga Kristina Kollander 

Joseph David Konschnik  Julie A. Kowalski  Mary Kay Krogull  Christian C. Krueger 

Restek Corporation 

Trace Analytics 

Eurofins 

Complete Phytochemical Solutions  Colorado Department of Public Health 

Heather Krug  Kevin Kubachka  Scott Kuzdzal  Karen L'Emperur  Markus Lacorn 

US FDA 

Shimadzu Scientific Instruments, Inc. 

Industrial Labs  R‐Biopharm AG 

Alex LaGory 

Kombucha Brewers International 

Kristie Laurvick, MS 

US Pharmacopeia (USP) 

John Lawry 

Covance 

Soo‐Kwang Lee 

FDA 

Qi Lin 

Abbott Nutrition R&D 

Alex Liu 

SCIEX 

Haiyan Liu 

Ocean Spray Cranberries, Inc. 

Bob Lockerman  Robert Lockerman  Daniel Lopez‐ferrer 

CEM Corporation  CEM Corporation  Thermo Scientific

SPSFAM Roster as of March 6, 2018 

CYNTHIA LUDWIG 

Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member 

AOCS 

Lifu Ma 

Certified Laboratories, Inc.  The Coca‐Cola Company  Friesland Campina Domo 

Farzaneh Maniei, MS 

Vicky Manti  Eric Marceau 

Canadian Food Inspection Agency (CFIA) 

Elaine Catherine Marley  Christina Marrongelli 

R‐Biopharm Rhone Ltd 

Medical University of South Carolina 

Katerina Mastovska  Mary T. McBride.  Barry V. McCleary 

Covance Laboratories  Agilent Technologies, Inc. 

Megazyme 

Katelin Merkh  Josh Messerly 

Lassonde Pappas 

Eurofins Nutrition Analysis Center  Keurig Green Mountain, Inc.  Mindak Professional Services, LLC  Alcohol & Tobacco Tax & Trade Bureau 

Paul Milne 

William Mindak  Armen Mirzoian 

Allen Misa 

Phenomenex 

Deepali Mohindra  Linda Monaci  Lisa Monteroso  Elizabeth Mudge  Cory J Murphy  Gary D. Niehaus 

Thermo Fisher Scientific 

National Research Council Of Italy 

3M Food Safety 

BCIT 

Canadian Food Inspection Agency  Crystal Diagnostics ‐ NEOMED 

Masayuki Nishimura, Sr. 

Shimadzu 

Gregory O. Noonan  Maria Ofitserova 

US FDA ‐ CFSAN 

Pickering Laboratories, Inc. 

Oliver Ou 

USDA FSIS LQAD 

Vincent Paez 

SCIEX 

Arun Paga 

Eurofins Central Analytical Lab 

Christine Parker 

FDA 

George Stanley Penson 

Cott Beverages 

Quang Son Pham 

Abbott 

Melissa Meaney Phillips 

NIST 

Tom Phillips  Tom Phillips  Tom Phillips 

MD Department Of Agriculture  MD Department Of Agriculture  MD Department Of Agriculture 

Curtis S. Phinney 

Curtis S. Phinney, CNS  Nestle Research Center 

Eric Poitevin  Bert Popping 

FOCOS ‐ Food Consulting Strategically 

Rick Reba  Jess Reed  Klaus Reif 

Nestle USA, Inc. 

University of Wisconsin‐Madison 

PhytoLab GmbH & Co., KG

SPSFAM Roster as of March 6, 2018 

John Reuther  Amanda Rigdon 

Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member 

Eurofins Central Analytical Lab 

Emerald Scientific 

Catherine A. Rimmer  Shauna F. Roman 

NIST 

Capstone Nutrition  Waters Corporation  SC Laboratories, Inc.  Abbott Laboratories 

Joe Romano 

Travis Ruthenburg  Louis M. Salvati, M.S. 

Andre Santos 

Agilent Technologies, Inc.  Atlantic Test Labs, Inc. 

Timothy Schnupp  Andre Schreiber  Brooke Schwartz 

SCIEX 

Rheonix Inc. 

Carl Schwarz  Jenny Scifres 

SCIEX 

USDA FSIS OPHS LQAD ALP 

Jennifer Sealey‐Voyksner 

ImmunogenX 

Tom Seipelt 

Abbott Nutrition 

Jeffrey Shippar  Kevin Shores 

Covance Laboratories 

Valspar  Neogen 

Amanda Kay Simon  Christopher Smith  Aniko M. Solyom  Kasi V. Somayajula  Katherine Stenerson  Cheryl Stephenson. 

The Coca‐Cola Company 

GAAS Analytical 

The Coca‐Cola Company 

MilliporeSigma 

Eurofins Central Analytical Laboratory 

Joan Stevens 

Agilent Technologies  Coca‐Cola Company  Covance Laboratories 

Rachel Stryffeler 

Mr. Darryl M. Sullivan 

Julie Sundgaard 

ROMER Labs Inc 

Alan Sutton 

GW Pharmaceuticals 

Christian Sweeney 

Cannabistry Labs 

John Szpylka 

Mérieux NutriSciences  Perrigo Nutritionals 

Steve Tennyson 

Nancy Thiex 

Thiex Laboratory Solutions LLC  LATU ‐ Chromatography And Mass  Spectrometry Department 

Marina Graciela Torres Rodriguez 

John Travis  Daina Trout  Justin Trout 

NSF International  Health‐Ade LLC 

Health‐Ade Kombucha 

Socrates Trujillo  Tomasz Tuzimski 

US FDA 

Medical University Of Lublin 

Harrie Van Den Bijgaart  Jolanta Vidugiriene  Sue (Shuimei) Wang 

Qlip BV 

Promega 

ITRI

SPSFAM Roster as of March 6, 2018 

Wayne Wargo  Ms. Jane Weitzel 

Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member  Member 

Abbott Nutrition  Biovail Corporation 

Walter Brent Wilson 

NIST  SCIEX 

Paul Winkler  Seth Wong  Laura Wood 

TEQ Analytical Labs 

NIST 

Beltsville Human Nutrition Research  Center, USDA‐ARS  Archer Daniels Midland Company 

Xianli Wu 

Jason Lynn Wubben 

Joshua Wurzer 

SC Labs 

Sudhakar Yadlapalli 

First Source Laboratory Solutions LLP 

Charles Yang 

Thermo Scientific  Nestle Nutrition 

Jupiter M. Yeung. 

Hong You 

Eurofins Scientific, Inc.  Coca‐Cola Company 

Chen Zhang 

Shanghai Entry Exit Inspection And  Quarantine Bureau 

Yao Zhou 

Patrick Martin Zoder, MS 

PALL Corporation 

Jerry Zweigenbaum 

Agilent Technologies, Inc. 

Scott G. Coates 

AOAC Staff  AOAC INTERNATIONAL 

OMB  Advisor 

Florida Department Of Agriculture And  Consumer Services 

Jo Marie Cook 

Christopher Dent  Dawn L. Frazier  Deborah McKenzie 

AOAC Staff  AOAC INTERNATIONAL  AOAC Staff  AOAC INTERNATIONAL  AOAC Staff  AOAC INTERNATIONAL

MARCH 12, 2018 GAITHERSBURG MARRIOTT WASHINGTONIAN CENTER 9751 WASHINGTONIAN BLVD., GAITHERSBURG, MD, 20878 Room: Salon C/D/E 1:00pm – 5:00pm ET Registration Opens at 12:00pm STAKEHOLDER PANEL ON STRATEGIC FOOD ANALYTICAL METHODS SPSFAM Chair: Erik Konings, Nestlé A G E N D A

Welcome and Introductions (1:00pm – 1:15pm) Jonathan Goodwin, AOAC and Erik Konings, Nestlé, SPSFAM Chair a. Policies and Procedures b. Approval of September 24, 2017 Minutes SPSFAM Activities Updates (1:15pm – 1:30pm) Erik Konings a. SPSFAM Expert Review Panel Updates

I.

II.

i. Allergens, Bisphenol-A, Cannabis, Heavy Metals, Kombucha, and Proanthocyanidins

III. SMPR Approval Presentations and Consensus* (1:30pm – 3:00pm) a. Sugar Working Group SMPR Presentations

John Szpylka, Mérieux NutriSciences and Nancy Thiex, Thiex Laboratory Solutions, LLC

Working Groups in Progress: Updates (3:15pm – 4:00pm) a. Cannabis Chair: Susan Audino, S.A. Audino & Associates b. Veterinary Drug Residues Chair: Joe Boison, University of Saskatchewan V. Future Topics of Interest for SPSFAM (4:00pm – 5:00pm) Group discussion about the need for additional standards across the industry.

IV.

a. Furan and Alkyl Furans in Coffee, Baby Foods, and Cereal Products b. Other Potential Topics

Adjourn (5:00pm)

VI.

Break: 3:00pm-3:15pm

*Item requires a vote This agenda is subject to change without notice

V5 2/22/2018

AOAC INTERNATIONAL Stakeholder Panel on Strategic Food Analytical Methods Working Group Sessions – March 13, 2018 12:00 p.m., Salon G

I. SPSFAM Sugars Working Group (12:00 pm – 1:00 pm) Co-Chairs: John Szpylka, Mérieux NutriSciences and Nancy Thiex, Thiex Laboratory Solutions

1. Welcome and Introductions 2. Review of March 2 Meeting a. Action Items 3. Low Lactose SMPR Development 4. Adjourn II. SPSFAM Veterinary Drugs Working Group (2:00 p.m. – 3:00 p.m.) Chair: Joe Boison, University of Saskatchewan 1. Welcome and Introductions 2. Review of Last Meeting a. Action Items 3. Veterinary Drug Residues SMPR Development 4. Adjourn

*Item requires a vote This agenda is subject to change without notice

V5 2/22/2018

AOAC Stakeholder Panel on Strategic Food Analytical Methods September 24, 2017, 8:00 a.m. – 1:30 p.m. ET Stakeholder Panel Meeting

STAKEHOLDER MEETING ATTENDEES ( present during all or part of the meeting ) Erik Konings, Nestlé (Chair) Bao Lei, Nestlé

Kathy Swartout, VUV Analytics John Szpylka, Mérieux NutriSciences Jing Tan, Abbott Nutrition Nancy Thiex, AAFCO Marina Torres, LATU Sherri Turnipseed, US FDA Harrie van den Bijgaat, Qlip BV Son Tran, National Institute for Food Control Jeroen van Soest, Eurofins Food Testing NL Martine vanGool, FrieslandCampina

Patricia Atkins, SpexCertiPrep

Han Li, China Alex Liu, SCIEX

Susan Audino, Audino & Associates LLC

Jacob Babu, MilkTestNZ

Lifu Ma, Certified Labs

Dongryeo Bae, US FDA/ORA/ARL

Ferry Maniei, The Coca-Cola Company Christina Marrongelli, Univ. of South Carolina Marla Marsh, Michigan Dept. of Agriculture Mary McBride, Agilent Technologies Patricia Meinhardt, R-Biopharm, Inc.

Charles Barber, US NIST Louis Bluhm, USDA FSIS

Joe Boison, CFIA

Hillel Brandes, MilliporeSigma Michele Bugayong, Abbott Nutrtion Esther Campos Gimenez, Nestlé Xu-Liang Cao, Health Canada Jo Marie Cook, Florida Dept of Agriculture Hans Cruijsen, FrieslandCampina Bob Clifford, Shimadzu

Josh Messerly, Eurofins

Paul Milne, Keurig Green Mountain

Tom Vennard, Covance Eric Verdon, ANSES

Armen Mirzoian, US TTB Brian Musselman, Ionsense

Ioannis Vrasidas, Eurofins Food Testing NL

Yasutaka Nishiyama, NH Foods Ltd. Maria Ofitserova, Pickering Laboratories Arun Paga, Eurofins Central Analytical Lab

Jian Wang, CFIA

Wayne Wargo, Abbott Nutrition Wade Whittington, Tyson Foods

Marcel de Vreeze, NEN-ISO Thierry Delatour, Nestlé

Melissa Phillips, US NIST

Paul Winkler, SCIEX Laura Wood, US NIST Jingcun Wu, Perkin Elmer

Khalil Divan, Thermo Fisher Scientific Jennifer Donnelson, VUV Analytics

Rick Reba, Nestlé

Klaus Reif, Phytolab GmbH & Co. KG

Aurelie Dubois, International Dairy Federation Lars Reimann, Eurofins

I-Lin Wu, US FDA

David Ellingson, Covance

John Reuther, Eurofins

Sudhakar Yadlapalli, First Source Laboratory

Tetsu Goto, CSC

Joe Romano, Waters Corporation Andre Santos, Agilent Technologies Brian Schaneberg, Starbuck Corporation Brook Schwartz, Rheonix, Inc. Tom Seipelt, Abbott Nutrition Keven Shores, Sherwin Williams Jayant Shringapure, Tyson Foods Kasi Somayajula, The Coca-Cola Company Angela Song, Abbott Nutrition Joan Stevens, Agilent Technologies Sidney Sudberg, Alkemist Labs Aniko Solyom, GAAS Analytical

Solutions

Cathy Halverson, US TTB

Charles Yang, Thermo Fisher Scientific Jinchuan Yang, Waters Corporation Zhou Yang, Eurofins Scientific, Inc. Michael Young, Waters Corporation Zhenfeng Yue, Shenzhen CIQ Zhiming Zhang Agilent Technologies

Philip Haselberger, Abbott Nutrition Thomas Hektor, R-Biopharm AG

Steve Holroyd, Fonterra

Ron Hunter, The Coca-Cola Company

Martha Jennens, Covance

Richard Jordan, Pacific Agricultural Laboratory Chris Smith, The Coca-Cola Company

Jie Zhang, Mead Johnson Linda Zhao, Abbott Nutrition

Brian Kirk, METTLER TOLEDO Estela Kneeteman, INTI

Hui Zhao, Covance Wei Zhu, Danone

Joseph Konschnik, Restek Corporation

Scott Krepich, Phenomenex

Garrett Zielinski, Covance

Mary Krogull, Eurofins Scientific, Inc. Hao Le, National Institute for Food Control

Donna Zink, AIM Research Enterprises, LLC

Darryl Sullivan, Covance

John Lee, Agilent Technologies

Hiroko Suzuki, Japan Food Research Laboratories

AOAC STAFF AND CONSULTANTS ( present during all or part of the meeting ) Scott Coates, Christopher Dent, Jennifer Diatz, Arlene Fox, Dawn Frazier, Jonathan Goodwin, Zerlinde Johnson, Deborah McKenzie, Tien Milor, Robert Rathbone,

Meeting Minutes I.

Welcome and Introductions

SPSFAM Chairman Erik Konings opened the meeting and led introductions at 8:05 am ET.

II.

SPSFAM Updates Konings asked for a motion to approve the meeting minutes from the March 13, 2017 SPSFAM Meeting Minutes. MOTION to approve the March 13, 2017 SPSFAM Meeting Minutes (Reba/Cook) 17 in favor, 0 opposed, 1 abstention. The motion passed. Konings then directed all participants to AOAC’s policies and procedures, advising that AOAC requires all participants to comply with the AOAC INTERNATIONAL Antitrust Policy Statement and Guidelines as well as all AOAC Policies and Procedures, found at www.aoac.org . He concluded his introduction with a presentation 1 regarding the status of SPSFAM and its various initiatives. a. Bisphenol-A (BPA) Konings then introduced Darryl Sullivan, Chair of the SPSFAM BPA Working Group. Sullivan took the floor with a presentation 2 describing the work of the BPA working group and a review of the background of BPA. Sullivan highlighted two comments that were submitted in response to the public comment period: • If the method is intended to measure free bisphenol A only then it should be stated as such. The phenolic groups of bisphenol A can react with beverage components (e.g. acids) to form esters, which are not captured unless they are first hydrolyzed. Response: Word “free” added to title • The phrase "grain-based dairy beverages" is sort of an oxymoron. If the beverages are grain- based they are not dairy, even though their name contains the word "milk". Response: Changed to “grain-based beverages.” Sullivan and Scott Coates reviewed these comments and determined that they could be added to the SMPR. Sullivan continued, explaining the method performance requirements set for this SMPR. After a brief discussion, a motion was made. SMPR Approval Presentations and Consensus

III.

1 SPSFAM Update Presentation (Konings) 2 SPSFAM BPA SMPR Approval Presentation (Sullivan)

MOTION to approve the Standard Method Performance Requirements ® for Determination of free Bisphenol A (BPA) in commercially packaged ready to consume carbonated and non- carbonated water and non-alcoholic beverages. (Szpylka/Yadlapalli). 17 in favor, 0 opposed, 1 abstention. The motion passed. 3 b. Cannabis in Chocolate Konings then introduced Susan Audino, Chair of the SPSFAM Cannabis Working Group. Audino took the floor with a presentation 4 on her group’s progress since the last meeting, primarily the development of an SMPR for Cannabis in Chocolate. The SMPR Consensus-based Reference method for use by trained technicians in a laboratory for routine quality assurance testing was put on screen and reviewed in detail. Numerous minor changes were made to the document. “Reference materials” was changed to “Examples of Reference Materials.” A link to the TDRM database was added under reference materials. A Shimadzu representative advised that they can also supply materials, so a reference to Shimadzu was also added under Reference Materials. After further discussion, a motion was made.

MOTION to accept the SMPR for Quantitation of Cannabinoids in Edible Chocolate Foods . 5 (Audino/Cook).

13 in favor, 0 opposed, 6 abstentions. The motion passed.

Audino concluded by updating the panel on the group’s progress on a pesticide residue SMPR. Audino explained that the SMPR is in progress and will be presented to SPSFAM for approval in March, 2018. Finally, Coates explained a memorandum 6 he and the working group had put together regarding the use of surrogate materials in cannabis testing. This memo applies to all Cannabis SMPRs developed to date.

IV.

Launch of New Working Groups a. Sugars

John Szpylka and Nancy Thiex. Co-Chairs of the sugars took the floor with their presentation 7 to launch the Sugars Working Group. The presentation proposed three separate fitness for purpose statements (leading to three SMPRs) for this project; one for sugars in animal feed, pet food, and human food; one for dietary fructan in animal feed, pet food and ingredients; and one for measuring lactose in dairy products. After some discussion and edits, the panel agreed to the following three fitness for purpose statements:

3 SPSFAM BPA SMPR as approved 9/24 4 SPSFAM Cannabis SMPR Presentation 5 SPSDAM Cannabis in Chocolate SMPR as approved 9/24 6 Coates Memo re: Cannabis Testing Materials 7 Launch Presentation: Sugar Working Group

1. Fitness for purpose for sugar: Individually measure free nutritional sugars, minimally: fructose, glucose, sucrose, maltose, lactose, and galactose found in ingredients and foods consumed by animals, pets, and humans. The analytical method must account for common interferences, such as sugar alcohols in these matrices. MOTION to accept the fitness for purpose as written (Thiex / Sudburg.) 17 in favor, 0 opposed, 2 abstentions. The motion passed. 2. Fitness for Purpose for Dietary Fructan in Animal Feed, Pet Food, and Ingredients: Measure total dietary fructan such as inulin and fructooligosaccharides in animal feed, pet food, and the corresponding ingredients. The method must distinguish this compound from interfering compounds such as free glucose, fructose, sucrose and other carbohydrates. MOTION to accept the fitness for purpose as written (Thiex / McCleary). 19 in favor, 0 opposed, 0 abstentions. The motion passed. 3. Lactose: Measure the amount of lactose in dairy products, including products containing daily ingredients that are low lactose or lactose-free. MOTION to accept (Szpylka / Yadlapalli), 19 in favor, 0 opposed, 0 abstentions. The motion passed. b. Veterinary Drugs Konings announced that Lei Bao of the AOAC China Section would start the discussion on veterinary drugs with a presentation. She reviewed the recent discussion on veterinary drugs at the last AOAC China section meeting, what is happening in China regarding vet drugs, and feedback of the Chinese stakeholders on this initiative, which was largely positive and emphasized the need for a method that can detect numerous pesticides. Joe Boison, Chair of the Veterinary Drugs Working Group, then took the floor with a presentation 8 to launch that group. Boison said the exact number of pesticides will be determined by the working group, but it will be at least 150. Boison then reviewed the background of the project, its significance, challenges and proposed timelines. He then recommended a fitness for purpose statement, which was discussed at length. The following wording was agreed to: The method should be applicable to the screening and identification of veterinary drugs (antibiotics, antiparasitics, anti-inflammatories & tranquilizers) in: ▪ raw milk, ▪ processed dairy powder ingredients (full-cream milk, fat-filled milk, skimmed milk, whey proteins, lactose, caseinate), ▪ meat including muscle, kidney, liver and fat (chicken, duck, turkey, beef, pork, lamb, veal), ▪ fish and seafood

▪ egg powders (whole, white and yolk), and ▪ infant formulae (regular and hydrolyzed). ▪ The method should be consistent with worldwide regulatory requirements.

8 Launch presentation: Veterinary Drugs

MOTION to accept the Veterinary Drug Residues fitness for purpose statement and launch the working group. (Boison/Audino) 20 in favor, 0 opposed, 0 abstentions. The motion passed. V. Adjourn

Konings thanked the stakeholders for their participation and adjourned the meeting at 1:00 pm ET.

Attachments:

1. SPSFAM Update Presentation

2. SPSFAM BPA SMPR Approval Presentation

3. SPSFAM BPA SMPR as approved 9/24

4. SPSFAM Cannabis SMPR Presentation

5. SPSFAM Cannabis in Chocolate SMPR as approved 9/24

6. Coates Memo re: Cannabis Testing Materials

7. Launch Presentation: Sugar Working Group

8. Launch presentation: Veterinary Drugs

These attachments are available upon request to SPSFAM@aoac.org

Status of SPSFAM First Action Official Methods of Analysis SM Expert Review Panels (ERPs)

Allergens ERP:- •

Detection and Quantitation of Selected Food Allergens ( AOAC SMPR 2016.002 ) o Two methods submitted, one method approved. o Call for Methods closed.

Bisphenol-A (BPA) ERP:- • Determination of Free Bisphenol-A in Commercially Packaged Ready to Consume Carbonated and Non-carbotated Water and Nonalcoholic Beverages ( AOAC SMPR 2017.018 )

o Four methods submitted, one method approved. o Accepting resubmissions from last ERP Meeting. o Call for Methods closed.

Cannabis ERP:- •

Quantitation of Cannabinoids in Plant Material ( AOAC SMPR 2017.001 ) o Two methods submitted, no methods approved. o Accepting resubmissions from last ERP meeting. o Call for Methods remains open. • Quantitation of Cannabinoids in Concentrates ( AOAC SMPR 2017.002 )

o One method submitted, no methods approved. o Accepting resubmissions from last ERP meeting. o Call for Methods open.

Heavy Metals ERP:- •

Quantitation of Arsenic Species in Food and Beverages ( AOAC SMPR 2015.006 ) o Five methods submitted, one method approved. o Call for Methods is closed. • Heavy metals in variety of foods and beverages ( AOAC SMPR 2012.07 ) o Six methods submitted, one method approved. o Call for Methods closed. Kombucha ERP:- • Determination of Ethanol in Kombucha ( AOAC SMPR 2016.001 )

o Eight methods submitted, two methods approved. o Accepting resubmissions from last ERP Meeting. o Call for Methods closed.

Proanthocyanidins (PAC) ERP:- •

Quantitation of Proanthocyanidin Content in Cranberry Products ( AOAC SMPR 2017.003) o Four methods submitted, no methods approved. o Accepting resubmissions from last ERP meeting. o Call for Methods remains open. • Identification of Type-A Proanthocyanidins in Cranberry Products ( AOAC SMPR 2017.004 )

o Two methods submitted, no methods approved. o Accepting resubmissions from last ERP Meeting. o Call for Methods open.

Watch the AOAC Website for these upcoming Calls for Methods!

• • •

• • •

Cannabis in Chocolate

Low Lactose Milk and Milk Products* Pesticide Residues in Cannabis* Veterinary Drug Residues in Food*

Sugars in Animal Feed, Pet Food, and Human Food* Fructans in Animal Feed, Pet Food, and Ingredients*

* Pending SMPR approval

AOAC STAKEHOLDER PANEL ON  STRATEGIC FOOD ANALYTICAL METHODS

Sugar Working Group – SMPR Presentation March 13, 2018 Co‐Chairs:

John Szpylka, M é rieux NutriSciences Nancy Thiex, Thiex Laboratory Solutions

Marriott Washingtonian Center,Gaithersburg, Maryland, USA

Fitness for Purpose As Agreed March 13, 2017

Fitness for purpose for sugar: Individually measure free nutritional sugars, minimally: fructose, glucose, sucrose, maltose, lactose, and galactose found in ingredients and foods consumed by animals, pets, and humans. The analytical method must account for common interferences, such as sugar alcohols in these matrices. Fitness for Purpose for Dietary Fructan in Animal Feed, Pet Food, and Ingredients: Measure total dietary fructan such as inulin and fructooligosaccharides in animal feed, pet food, and the corresponding ingredients. The method must distinguish this compound from interfering compounds such as free glucose, fructose, sucrose and other carbohydrates. Lactose: Measure the amount of lactose in dairy products, including products containing daily ingredients that are low lactose or lactose-free .

SPSFAM Sugar Working Group Work To Date

• 6 teleconferences (December 2017 – March 2018) • 2 SMPR Drafts Completed • Public comment period (February, 2018) • SMPRs made ready for SPSFAM review and approval 

SPSFAM Sugar Working Group Members

Merieux NutriSciences

JohnSzpylka ASINALSAS Thermo Fisher

NancyThiex

Thiex LaboratorySolutions

BelisarioAcevedo

Douglas LloydHolt

Dr Pepper SnappleGroup

MariuszSliwinski Georgina Smyth Kathryn Stanley MoniqueSteegmans

Instytut Innowacji PrzemysluMleczarskiego Sp. zo.o.

Parul Angrish SeanAustin

JeffG.Horst

Agri‐King Inc

PublicAnalysts Laboratory

NestleResearchCenter

Martha Jennens Diana C.Kavolis Erik J.M.Konings DanaA.Krueger Markus Lacorn Cheryl L.Lassitter ScottKrepich

CovanceLaboratories TheHersheyCompany NestleResearchCenter

ArcherDanielsMidlandCompany

KnukErikBachKnudsen

Aarhus University

TienseSuikerraffnaderji Analytical Services

Charles A.Barber

NIST

Hiroko Suzuki Kathy Swartout

Japan FoodResearch Laboratories

Daniel Berg

Covance

Phenomenex

VUVAnalytics

SnehD.Bhandari Sharon L.Brunelle

MerieuxNutriSciences

Krueger Food Laboratories, Inc.

NaimTahiri

Birra Peja

AOAC INTERNATIONAL (Contractor)

R‐BiopharmAG

Richard Ten Eyck

AAFCO

KaitlinCahill JaneCaldwell

Q Laboratories, Inc. MidwestLaboratories DanoneResearch MaxxamAnalytics

NOAA ‐DOC,NMFS,NSIL

Rodriguez

LATU ‐ChromatographyAndMass SpectrometryDepartment

Sookwang Lee

FDA

Peter J.Van Soest

Cornell University CovanceLaboratories

ChristianCampargue

Alex Liu Kai Liu

SCIEX

TomVennard

FranceCho

EurofinsNutritionAnalysis Center Roberta Vidal

Pepsico

MarkW.Collison

ArcherDaniels MidlandCompany BozenaD. Lusiak

NestlePurina

Hedegaard

IDF

Hans Cruijsen ManishaDas

FrieslandCampina

Eva Lynch

RockRiver Laboratory CovanceLaboratories

Ioannis Vrasidas Yannis Vrasidas

Eurofins Food TestingTheNetherlands Eurofins Food TestingNetherlands Medallion Labs /General Mills RWANDABUREAUOF STANDARDS

U.S.FDA

KaterinaMastovska BarryV.McCleary GastonM.Mercier

Marcel DeVreeze JenniferDonelson Dr.AurelieDubois

Institute (NEN) VUVAnalytics

Megazyme

PaulWehling

Canada

THEOWICLEFKAGISHA

International Dairy Federation UnileverResearchVlaardingen

PierreL.Metra

MerieuxNutriSciences

PaulWinkler RonaldWinter BryanWirthwine

SCIEX

Dr.GustaafS.Duchateau DavidEllingson,M.S.

LuciaMonti

CREA‐ZA

FDA

CovanceLaboratories Metrohm‐Peak, Inc

Hari Narayanan SalvatoreParisi

MetrohmUSA Inc

Q Laboratories, Inc.

Mr. JayGandhi

COIFAssociation, Italy MerieuxNutriSciences

DougWolfe LauraWood

McCoy&McCoy Laboratories, Inc.

Dr.FrankGottsleben JonathanHache Ms.GaleHagood MohamedHamad ThomasHektor RyanHoefling Dr.SteveHolroyd PhilipAndrewHaselberger

AntonPaarOptoTecGmbH

GiampaoloPerinello

NIST

Canadian Food InspectionAgency MelissaMeaneyPhillips

NIST

DavidC.Woollard Sudhakar Yadlapalli

EurofinsNewZealand Laboratories FirstSourceLaboratory Solutions LLP

Mississippi StateUniversity Microbac Laboratories Inc.

Miachael Raessler LarsM.Reimann CatherineA.Rimmer Alejandra Rodriguez

MPI Biochemie

Eurofins Scientific, Inc.

XunYan

AmwayCorp

AbbottNutrition R‐BiopharmAG

NIST

JinchuanYang

Waters Corporation

InternationalDairy Federation ZhengYang

Cargill

ICMTechDevelopment

JoeRomano

Waters Corporation

GuhongZhao

Northland Laboratories

Fonterra Co‐operativeGroup Ltd.

Sandra Salleres

BiolanMicrobiosensors

Background: Sugar

• For animal feeds, current sugar analyses typically rely on extraction, acid  hydrolysis of solubilized carbohydrates, and measurement as reducing sugars,  or measurement of total extracted carbohydrates using condensation  reactions. – Inappropriately includes oligosaccharides and other solubilized  carbohydrate in the sugar measurement.  Also for some feeds, active  amylases digest carbs into sugars thus artificially increasing the measured  sugar amount. • For measuring individual sugars in foods, traditional HPLC methods have  difficulty – separating some monosaccharides from each other, and – resolving sugars from interfering compounds such as sugar alcohols

Background: Sugar

• Currently there are no approved methods for individually measuring mono- and di-saccharides in animal feed or pet food. • RI methods do not achieve desired level for low level sugar guarantees. • AOAC Official Method 974.06 Sugars (Total) in Animal Feed Modified Fehling Solution Method • Several issues on accounting for different sugars being present

Sugar SMPR Key Points

Applicability • Individually measure free nutritional sugars, minimally:  fructose, galactose, glucose, sucrose, maltose, and lactose  found in selected ingredients and foods consumed by  animals, pets, and humans.  The analytical method must  account for potential interferences (see list provided) in these  matrices.  Methods that target a narrower matrix scope will  also be considered when appropriate.  

Sugar SMPR Key Points

Potential Interferants • Non‐target  mono, di‐ and tri‐ saccharides (e.g., maltotriose interfering  with maltose) • Enzymatic activity (amylase, invertase) • Hydrolytic activity (e.g., acidic) • Organic acids • Glucose with higher degrees of polymerization • Sugar alcohols, such as: glycerol, erythritol, xylitol, sorbitol, mannitol,  maltitol, lactitol, isomalt • Hydroxylated compounds (non‐targeted carbohydrates, sugar alcohols,  sugar acids, sucralose, etc.) • Salts, such as sodium chloride • Amine containing compounds (glucosamine HCl, amino acids, peptides,  glycoproteins, etc.)

Sugar SMPR Key Points

• Method data packages must include performance data  covering the method claimed matrices. A useful tool can be  found in food pyramid concept described in Annex F of  Appendix F the AOAC Official Methods of Analysis. Matrix  variations in non‐sugar carbohydrate and moisture content  must be investigated and data provided.  For example, using  the food pyramid in sector 7, a matrix may be either high in  sugar or high in non‐sugar carbohydrates (e.g., starch, sugar  alcohols), each of which may affect method performance.   • Method data packages must include relevant data regarding  interferences and instabilities.

Comments Submitted for Sugar SMPR

Comment

Response

There should be significant evaluation  of the method for finished pet foods  before it is accepted, to avoid significant  problems with interferants.  Otherwise,  there are quite a few common  ingredients in pet food which would  cause interference. Can you please provide clarification in  the SMPR if the method must be  written to encompass all sugars in one?

SMPR states “Method data packages  must include relevant data regarding  interferences and instabilities”, and  includes a list of classes of potential  interents with clear examples.   Recommend no change to SMPR . The SMPR  implies a single method to  measure the at least six free sugars.   The working group agreed to a  minimum set of six free sugars.  Recommend no change to SMPR . The minimum number of sugars is  clearly defined" . . . minimally: fructose,  galactose, glucose,sucrose, maltose,   and lactose . . .“. Recommend no change  to SMPR .

SMPR should state somewhere that  submission of methods that only cover  a subgroup of sugars in selected  matrices are also of interest. 

Comments Submitted for Sugar SMPR

Comment

Response

Sample stability needs to be evaluated  for extraction time (depending on  solution) and also overall shelf life after  extraction due to enzymes and  microbial activities in these matrices.  

A paragraph was added to Section 7.  “Method data packages must include  relevant data regarding interferences  and instabilities, such as listed in Table  2.   Data packages must include data to  demonstrate that the sugar  composition in the extract is a  representation of the true sugar  composition in the food product and  assess the stability of extracts over the  duration of the test.“

Comments Submitted for Sugar SMPR

Comment

Response

No change but an ERP should get some  guidance when judging a method to be  in accordance to this SMPR. Otherwise  an endless discussion would occur  which is not due to a non‐validated  method but to an unclear SMPR. If a method developer submits a  method for sucrose‐glucose‐fructose  quantification in bread and Cookies, it is  up to a method user if this "narrower  matrix range" is appropriate or not.  I am concerned that there is no section  for reference materials in this SMPR.

1) If the submitted method is applicable  only to a subset of foods or feeds, that  information must be clearly described  to allow ERP deliberation if the method  satisfies Stakeholder needs. 2) As written, the minimum number of  sugars that must be measured by a  method is six  regardless of the  "narrower matrix scope" clause. Recommend no change to SMPR . This was an oversight by the working  group.  A reference material section was  added as Section 8 and includes  example Reference Materials Appendix  F in OMA.

Method Performance Requirements: Sugar

Recovery, repeatability and reproducibility parameters * Analytical range 0.1% ‐ 5%

5 % ‐ 50 % 50 % ‐ 100%

Recovery, %

90 ‐ 110

95‐105

97 – 103

RSDr, %

≤7

≤5

≤3

RSDR, % ≤4 * Reported as the individual sugars (fructose, glucose, sucrose, maltose,  lactose and galactose. ≤10 ≤8

Discussion?

Motion for Sugar SMPR

Move to approve the Standard  Method Performance Requirements  (SMPRs) for Sugars in Animal Feed,  Pet Food, and Human Food 

Background: Dietary Fructans

• Dietary fructan is water‐soluble class encompassing oligosaccharide  and polysaccharide carbohydrates – Comprised predominantly of DP>3 fructose molecules and containing no  more than one‐third glucose molecules. • This polymer of fructose molecules may or may not contain a terminal  glucose molecule, and includes inulin and FOS. – Fructans are not digested by enzymes in the small intestine but can be  fermented in the large intestine. • Nutritionally and compositionally distinct from other carbohydrates  such as sugars, starch, and other forms of dietary fiber.  • Primary sources of fructan are cool season grasses, chicory, and  onions.

Background: Fructans

Currently two AOAC methods for fructan in foods (999.03 and 997.08); however, not fit-for-purpose for animal food. • Acid hydrolysis is more effective than use of inulinase (effective on inulin) for analysis of fructans in cool season grasses. • Hydrolysis of current methods may be modified for use in animal food

Fructans SMPR Key Points

Applicability • Measure total dietary fructan, such as inulin, levan, branched fructans, agavins (agave fructans) and fructooligosaccharides, in animal feed, pet food, and the corresponding ingredients. The method must be capable of distinguishing this compound from interfering compounds such free glucose, fructose, sucrose and other polysaccharides.

Fructans SMPR Key Points

Validation Guidance • Method data packages must include performance data covering the method claimed matrices. A useful tool can be found in food pyramid concept described in Annex F of Appendix F the AOAC Official Methods of Analysis. • Method data packages must include relevant data regarding interferences from other carbohydrates. Method developers are responsible for assessing interferences with their method.

Comments Submitted for Fructans SMPR

• A typo was corrected in the operating range and LOQ.   Corrected to: • Operating range 0.2% ‐ 100 % (2g/kg to 1000 g/kg) of dry  matter • Limit of quantitation (LOQ)* 0.2% (2g/kg) of dry matter

• No additional comments received.