AOAC ERP MICRO AUGUST 2018
OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only
1 2
Table 2b. Inclusivity Study Results
3 4 5 6 7 8 9
#
Listeria species
Reference
Origin
Level tested 1.8e6 CFU/mL 2.65e5 CFU/mL 3.5e7 CFU/mL 1.95e7 CFU/mL McFarland 1 *
MDA2LIS
51 fleischmanii subsp. coloradensis
+
FSL S10-1203 Running water, FL
52 marthii
FSL S4-120 FSL F6-969 FSL F6-971
Pristine environment, forest, NY
+ + + +
53 cornellensis 54 grandensis
Water, CO Water, CO
55 riparia
FSL S10-1204 Running water, FL
10
• Table 2b are Listeria strains analyzed at 3M St. Paul, MN laboratory
• Cultures 51-54 were obtained from the laboratory of Dr. Martin Wiedmann at Cornell University, NY. Frozen suspensions were thawed and streaked to 11 sheep blood agar. Single isolated colonies were picked and sub-cultured in 10 mL Demi Fraser broth with FAC overnight at 37 ± 1°C. The cultures were 12 diluted with fresh Demi Fraser broth with FAC before testing using the 3M MDA2LIS kit. 13 • Culture 55 was also obtained from Cornell. The frozen suspension was thawed and streaked to sheep blood agar. This organism did not grow in broth at 14 37°C or at lower temperatures, therefore a McFarland 1* suspension (approximately 3e8 CFU/mL) was prepared from a colonial isolate prior to MDA2LIS 15 testing. 16 • FSL = Food Safety Laboratory Cornell University, NY
17 18 19
Table 3. Exclusivity Study Results
Inoculation level (cfu/ml) 1,0 x 10 4 (opacity +) <2,0x10 3 (opacity +) <2,0x10 3 (opacity+) 7,2x10 4
3M Molecular Detection Assay 2 – Listeria
Genus
Species cereus circulans coagulans
Reference
Origin
1 Bacillus 2 Bacillus 3 Bacillus 4 Bacillus 5 Bacillus 6 Bacillus 7 Bacillus 8 Bacillus
Ad 465 Ad 760 Ad 731 Ad 978 Ad 762 Ad 765 Ad 284 Ad 726 Ad 369 Ad 149 89L326 86L126 Ad 380 Ad 482 Ad 473 Ad 137
Salmon Terrine Vegetables Dairy product Dairy product
- - - - - - - - - - - - - - - - - - -
licheniformis
5,6x10 4 2,0x10 4 1,7x10 5 6,8x10 4 8,5x10 5
Milk
mycoïdes
Vegetables Ready-to-eat Egg product
pseudomycoides
pumilus
weihenstephanensis
9 Brochothrix 10 Brochrotrix 11 Carnobacterium 12 Carnobacterium 13 Enterococcus 14 Enterococcus 15 Lactobacillus 16 Lactobacillus 17 Lactobacillus 18 Lactobacillus 19 Lactococcus
EN 15129
Trout
thermosphacta
CIP 102920T Environment AOAC Research Institute Expert Review Panel Use Only <2,0x10 3 (opacity +) >2,0x10 3 (opacity +) <2,0x10 3 (opacity +) <2,0x10 3 (opacity +) CIP 101029 T Unknown Raw milk Ham Soft cheese (Vacherin) Ham
campestris divergens piscicola
durans faecalis brevis curvatus
1,6x10 5 1,4x10 5 1,7x10 5 1,2x10 5 4,9x10 5
Delicatessen Tomatoes juice
fermentum
Ham
sakei
>2,0x10 3 (opacity +)
Dairy product
lactis subsp cremoris
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