AOAC ERP MICRO AUGUST 2018

OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only

INCLUSIVITY

3M MDA 2 Demi Fraser broth 24h at 37°C

Inoculation level (cfu/225ml)

Confirmation 100µl ALOA Palcam

Strain

Species

Reference Origin

MDA2 - Listeria monocytogenes

43 Listeria 44 Listeria 45 Listeria 46 Listeria 47 Listeria 48 Listeria 49 Listeria 50 Listeria

monocytogenes Ad270 monocytogenes Ad272 monocytogenes Ad273 monocytogenes Ad274 monocytogenes Ad534 monocytogenes Ad544 monocytogenes Ad546 monocytogenes Ad623

Fermented sausage Fermented sausage Cured delicatessen

42 56 27 40 54 43 83 50

+ + + + + + + +

H+ H+ H+ H+ H+ H+ H+ H+

+ + + + + + + +

Ready-to-eat food (Asiatic meal)

Fruits Onion Flour

Bread crumbs

All the strains are wild strains isolated in Adria Developpement , Quimper, France. 1 Ad = Adria 2 A= Adria 3 C= Origin from Meat Meat products 4 M = Origin from Fish and Seafood 5 L = Origin from Milk and Dairy products 6 E = Origin from Productionenvironment 7 H+ = Phosphy-Lipolysis halo

8 9

10 11 12

EXCLUSIVITY AOAC Resea ch Institute Expert Review Panel Use Only Reference Origin Inoculation level (cfu/ml) 3M MDA2 – Listeria monocytogenes

Table 3. Exclusivity Study Results

Strain

Species

3.2 x 10 5

1 Listeria

Ad 1198

Smoked salmon

-

grayi