AOAC ERP MICRO AUGUST 2018
OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only
Table 4a: Independent Study - Matrices and Inoculating Organisms
1
Inoculating Organism (Culture Condition) L. monocytogenes ATCC 1 7644 (heat-stressed) L. monocytogenes ATCC 1 19116 (heat-stressed) L. monocytogenes CWD 2 1554 (heat-stressed) L. monocytogenes ATCC 1 19114 L. monocytogenes ATCC 1 19112 &
MDA2 Testing Time Points
# of Replicates
Reference Method (Enrichment) USDA/FSIS MLG 8.09 (UVM) USDA/FSIS MLG 8.09 (UVM)
Matrix
Target Inoculum Level
0 CFU/Test Portion
5
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
Beef Hot Dogs
24 hours
5 5
0 CFU/Test Portion
Deli Turkey
24 hours
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
5 5
0 CFU/Test Portion
AOAC 993.12 (Selective Enrichment) AOAC 993.12 (Selective Enrichment) AOAC 993.12 (Selective Enrichment)
Queso Fresco
24 hours
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion 0 CFU/Test Portion 0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
5 5 5 5
Vanilla Ice Cream
20
24 hours
0 CFU/Test Portion
4% Milk Fat Cottage Cheese
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
24 hours
L. welshimeri ATCC 1 35897
5 5
0 CFU/Test Portion
FDA/BAM Chapter 10 (BLEB)
L. monocytogenes FSL 3 J1-049
Whole Melon
26 hours
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
5
Lot 1 & Lot 2
20 & 20
USDA/FSIS MLG 8.09 (UVM)
Raw Chicken Leg Pieces
Naturally Contaminated
28 hours
0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/4” x 4” (100 cm2) ~50 CFU/4” x 4” (100 cm2) ~100 CFU/4” x 4” (100 cm2) 0 CFU/1” x 1” (5 cm2)
5
FDA/BAM Chapter 10 (BLEB)
Romaine Lettuce
L. monocytogenes ATCC 1 49594
20
24 hours
5 5
24 hours AOAC Research Institute Expert Review Panel Use Only AOAC 993.12 (Selective Enrichment) 20 5 5 24 and 26 hours USDA/FSIS MLG 8.09 (UVM) 20
3% Chocolate Whole Milk
L. monocytogenes ATCC 1 BAA-751
L. monocytogenes ATCC1 19117
Sealed Concrete
5
L. monocytogenes ATCC1 51782& L. seeligeri ATCC1 35967& 10x Enterococcus faecalis ATCC1 29212
5
USDA/FSIS MLG 8.09 (UVM)
~50 CFU/1” x 1” (5 cm2)
20
24 and 26 hours
Plastic
~100 CFU/1” x 1” (5 cm2)
5
2
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