AOAC ERP MICRO AUGUST 2018

OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only

Table 4a: Independent Study - Matrices and Inoculating Organisms

1

Inoculating Organism (Culture Condition) L. monocytogenes ATCC 1 7644 (heat-stressed) L. monocytogenes ATCC 1 19116 (heat-stressed) L. monocytogenes CWD 2 1554 (heat-stressed) L. monocytogenes ATCC 1 19114 L. monocytogenes ATCC 1 19112 &

MDA2 Testing Time Points

# of Replicates

Reference Method (Enrichment) USDA/FSIS MLG 8.09 (UVM) USDA/FSIS MLG 8.09 (UVM)

Matrix

Target Inoculum Level

0 CFU/Test Portion

5

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

Beef Hot Dogs

24 hours

5 5

0 CFU/Test Portion

Deli Turkey

24 hours

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5 5

0 CFU/Test Portion

AOAC 993.12 (Selective Enrichment) AOAC 993.12 (Selective Enrichment) AOAC 993.12 (Selective Enrichment)

Queso Fresco

24 hours

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion 0 CFU/Test Portion 0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5 5 5 5

Vanilla Ice Cream

20

24 hours

0 CFU/Test Portion

4% Milk Fat Cottage Cheese

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

24 hours

L. welshimeri ATCC 1 35897

5 5

0 CFU/Test Portion

FDA/BAM Chapter 10 (BLEB)

L. monocytogenes FSL 3 J1-049

Whole Melon

26 hours

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5

Lot 1 & Lot 2

20 & 20

USDA/FSIS MLG 8.09 (UVM)

Raw Chicken Leg Pieces

Naturally Contaminated

28 hours

0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/ Test Portion 0.2-2 CFU/ Test Portion 2-5 CFU/ Test Portion 0 CFU/4” x 4” (100 cm2) ~50 CFU/4” x 4” (100 cm2) ~100 CFU/4” x 4” (100 cm2) 0 CFU/1” x 1” (5 cm2)

5

FDA/BAM Chapter 10 (BLEB)

Romaine Lettuce

L. monocytogenes ATCC 1 49594

20

24 hours

5 5

24 hours AOAC Research Institute Expert Review Panel Use Only AOAC 993.12 (Selective Enrichment) 20 5 5 24 and 26 hours USDA/FSIS MLG 8.09 (UVM) 20

3% Chocolate Whole Milk

L. monocytogenes ATCC 1 BAA-751

L. monocytogenes ATCC1 19117

Sealed Concrete

5

L. monocytogenes ATCC1 51782& L. seeligeri ATCC1 35967& 10x Enterococcus faecalis ATCC1 29212

5

USDA/FSIS MLG 8.09 (UVM)

~50 CFU/1” x 1” (5 cm2)

20

24 and 26 hours

Plastic

~100 CFU/1” x 1” (5 cm2)

5

2