AOAC ERP MICRO AUGUST 2018
OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only
Table 4a: Independent Study - Matrices and Inoculating Organisms (Continued)
1 2
Inoculating Organism (Culture Condition) L. monocytogenes ATCC 19118 & 10x Enterococcus faecium ATCC 19434
MDA2 Testing Time Points
# of Replicates
Reference Method (Enrichment)
Matrix
Target Inoculum Level
0 CFU/1” x 1” (5 cm2)
5
USDA/FSIS MLG 8.09 (UVM)
24 and 26 hours
Stainless Steel
~50 CFU/1” x 1” (5 cm2)
20
0 CFU/4” x 4” (100 cm2)
5
3 4
1 ATCC - American Type Culture Collection 2 CWD - University of Vermont
3 FSL - Cornell University 5 * Natural contamination was present during the background screen: 2 separate lots evaluated (20 replicates each) 6 7 8 9 10 Table 4b: Internal Study - Matrices and Inoculating Organisms 11 Matrix Inoculating Organism (Culture Condition) Target Inoculum Level # of Replicates MDA2 Testing Time Points
Reference Method (Enrichment) USDA/FSIS MLG 8.09 (UVM) ISO 11290-1/A1 (Half/Demi Fraser)
0 CFU/Test Portion
5
Raw chicken leg pieces
L. monocytogenes Ad 1 668
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
28 hours
5 5 5 5 5 5
0 CFU/Test Portion
Bagged raw spinach
L. monocytogenes Ad 543
24 hours
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion 0 CFU/Test Portion 0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
FDA/BAM Chapter 10 (BLEB)
Cold smoked salmon
L. monocytogenes Ad 670
20
24 hours
0 CFU/Test Portion
Cold smoked salmon
ISO 11290-1/A1 (Half/Demi Fraser)
L. monocytogenes Ad 670
24 hours
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
5
12 13
AOAC Research Institute Expert Review Panel Use Only
1 Ad – Adria Developpement Culture Collection
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