AOAC ERP MICRO AUGUST 2018

OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only

Table 7: Inoculum Summary Table for Whole Melons and Environmental Surfaces Matrix Whole Melons Inoculating Organism Listeria monocytogenes FSL J1-049 Low-Inoculum Level CFU a /Melon b 7 High-Inoculum Level CFU a /Test Melon b 55 Matrix Stainless Steel Inoculating Organism Listeria monocytogenes ATCC 19118 Enterococcus faecium ATCC 19434 Low-Inoculum Level CFU a /Test Area c 42 530 High-Inoculum Level CFU a /Test Area c 400 4000 Matrix Sealed Concrete Inoculating Organism Listeria monocytogenes ATCC 19117 Low-Inoculum Level CFU a /Test Area c 35 High-Inoculum Level CFU a /Test Area c 360 Matrix Plastic Inoculating Organism Listeria monocytogenes ATCC 51782 Enterococcus faecalis ATCC 29212 Low-Inoculum Level CFU a /Test Area d 42 540 High-Inoculum Level CFU a /Test Area d 420 3600 a CFU: aliquots of the inocula were plated in triplicate onto TSA and averaged b Test Area: Whole Melon

c Test Area: 4” x 4” Surface Area d Test Area: 1” x 1” Surface Area

AOAC Research Institute Expert Review Panel Use Only