AOAC ERP MICRO AUGUST 2018
OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only
Table 5b: Internal Study - Aerobic Plate Count and Background Results (Prior to Inoculation)
Matrix (Test Portion)
APC 1 (CFU/g) Background Listeria
Raw Chicken Leg Pieces (25 g) Bagged Raw Spinach (25 g) Cold Smoked Salmon (25 g)
5.4 x 10 5
None Detected
8.0 x 10 6
None Detected
4.0 x 10 2
None Detected
1 APC conducted in accordance with FDA/BAM Chapter 3 2 Listeria species screen conducted following the USDA/FSIS MLG 8.09 guidelines 3 Listeria species screen conducted following the FDA/BAM Chapter 10 guidelines 4 Listeria species screen conducted following the ISO 11290-1/1A guidelines
Table 6: Inoculum Heat Stress Results
Test Portion Size
Matrix
Inoculating Organism
Agar
CFU/ g 1.8 x 10 8 7.5 x 10 7 2.6 x 10 9 8.2 x 10 8 2.6 x 10 9 7.1 x 10 8 2.6 x 10 9 6.8 x 10 8
Percent Injury
TSA
Listeria monocytogenes ATCC 7644 Listeria monocytogenes ATCC 19116 Listeria monocytogenes ATCC CWD 1554 Listeria monocytogenes ATCC 7644
Beef Hot Dogs 1
25 g
58.3 %
MOX
TSA
Deli Turkey
125 g
68.5 %
MOX
TSA
Queso Fresco
25 g
72.7%
MOX
TSA
Beef Hot Dogs 2
25 g
73.8%
MOX
1 - Testing for Robustness and Lot to Lot 2 - Matrix Study TSA: Trypticase soy agar MOX: modified Oxford agar
AOAC Research Institute Expert Review Panel Use Only
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