AOAC ERP MICRO AUGUST 2018

OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only

Table 5b: Internal Study - Aerobic Plate Count and Background Results (Prior to Inoculation)

Matrix (Test Portion)

APC 1 (CFU/g) Background Listeria

Raw Chicken Leg Pieces (25 g) Bagged Raw Spinach (25 g) Cold Smoked Salmon (25 g)

5.4 x 10 5

None Detected

8.0 x 10 6

None Detected

4.0 x 10 2

None Detected

1 APC conducted in accordance with FDA/BAM Chapter 3 2 Listeria species screen conducted following the USDA/FSIS MLG 8.09 guidelines 3 Listeria species screen conducted following the FDA/BAM Chapter 10 guidelines 4 Listeria species screen conducted following the ISO 11290-1/1A guidelines

Table 6: Inoculum Heat Stress Results

Test Portion Size

Matrix

Inoculating Organism

Agar

CFU/ g 1.8 x 10 8 7.5 x 10 7 2.6 x 10 9 8.2 x 10 8 2.6 x 10 9 7.1 x 10 8 2.6 x 10 9 6.8 x 10 8

Percent Injury

TSA

Listeria monocytogenes ATCC 7644 Listeria monocytogenes ATCC 19116 Listeria monocytogenes ATCC CWD 1554 Listeria monocytogenes ATCC 7644

Beef Hot Dogs 1

25 g

58.3 %

MOX

TSA

Deli Turkey

125 g

68.5 %

MOX

TSA

Queso Fresco

25 g

72.7%

MOX

TSA

Beef Hot Dogs 2

25 g

73.8%

MOX

1 - Testing for Robustness and Lot to Lot 2 - Matrix Study TSA: Trypticase soy agar MOX: modified Oxford agar

AOAC Research Institute Expert Review Panel Use Only