AOAC ERP MICRO AUGUST 2018

AOAC Official Methods Program  Expert Review Panel for Microbiology for Foods and Environmental Surfaces 

ER 1 ER 2 ER 3 ER 4 ER 5

Bird et al.: Journal of AOAC international Vol. 99, no. 4, 2016 p. 997

NA

I am interpreting "reference material" as the inclusivity/exclusivity tables.

Reference cultures

Not aware of any. What type of single laboratory validations were used for this method [i.e, Harmonized PTM, Precollaborative, Independent,  Collaborative, etc.] and where is it referenced in the method documentation? ER 1 Harmonized AOAC Performance Tested Method SM (PTM) study. The objective of the PTM study was to demonstrate that the 3M MDA 2  – Salmonella method could detect Salmonella in select food matrixes and environmental surfaces as claimed by the manufacturer ER 2 Collaborative. AOAC Official Method 2016.01 ER 3 Harmonized PTM. PTM method 091501 starting page 207 AOAC ERP booklet August 2018 ER 4 Harmonized PTM ER 5 PM certification number 091501 Are the approved validation protocols available and documented? ER 1 Yes ER 2 Yes ER 3 Yes ER 4 Yes ER 5 Yes Is the method documentation available that demonstrates Reproducibility/Uncertainty and Probability of Detection? If so, please cite  the reference. ER 1 ER 2 ER 3 ER 4 ER 5 STATISTICAL REVIEW: Is the documentation for the statistical review prior to AOAC First Action Official Methods status available and  completed? ER 1 yes ER 2 Yes ER 3 yes. ER 4 Yes ER 5 yes Please cite below the OMA Appendicies, Community Guidance, or  SMPR by which this method was reviewed against. ER 1 For the collaborative study, the 3M MDA 2 – Salmonella method was compared to the USDA/FSIS MLG reference method for raw ground  beef and to the FDA BAM reference method for creamy peanut butter.  Official Methods of Analysis (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, Appendix J, http:// www.eoma.aoac.org/app_j.pdf Based upon the feedback submitted, are there any recommended changes to the AOAC First Action method as written? ER 1 No ER 2 No ER 3 No ER 4 No ER 5 No If yes, please explain and note whether the method author has addressed those concerns. ER 2 ER 3 ER 4 Appendix J: AOAC INTERNATIONAL Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces Appendix J. Appendix J ER 5

ER 1 ER 2 ER 3 ER 4 ER 5 ER 1 ER 2

There was no feedback information included

Has the method author addressed any specific AOAC Final Action requirements as noted by the ERP, if any?

yes

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